中国中西医结合急救杂志
中國中西醫結閤急救雜誌
중국중서의결합급구잡지
Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care
2015年
6期
611-614
,共4页
陈彤%闫桂虹%邢国胜%杨雅琼%徐瑾
陳彤%閆桂虹%邢國勝%楊雅瓊%徐瑾
진동%염계홍%형국성%양아경%서근
鱼腥草%兔%膀胱冲洗%尿路感染
魚腥草%兔%膀胱遲洗%尿路感染
어성초%토%방광충세%뇨로감염
Herba houttuyniae%Rabbit%Bladder irrigation%Urinary tract infection
目的：观察鱼腥草液膀胱冲洗对兔膀胱感染模型的抑菌作用，为防治尿路感染提供新的方法。方法选择雄性新西兰长耳大白兔20只，按随机数字表法分为庆大霉素对照组和3个不同浓度鱼腥草组，每组5只。采用导尿管向兔膀胱注入大肠埃希菌液1 mL（105 cfu/mL）夹闭导尿管1 h的方法复制兔膀胱感染模型；鱼腥草组分别向膀胱注入0.25、0.50、1.00 g/mL鱼腥草液50 mL，对照组向膀胱注入160 kU庆大霉素。于制模前及制模后3、5、7、9 d取兔耳血，检测各组血白细胞计数（WBC）、中性粒细胞比例（N%）；同时取中段晨尿检测菌尿阳性率和尿WBC阳性率；实验结束后处死动物取膀胱组织，光镜下观察病理学改变。结果制模前各组动物菌尿和尿WBC均为阴性，血常规WBC、N%均在正常范围。制模后3 d各组菌尿阳性率和尿WBC阳性率均达到100%；制模后随着治疗时间延长，各组菌尿阳性率和尿WBC阳性率逐渐下降，5 d时鱼腥草0.50 g/mL和1.00 g/mL组已出现统计学意义（菌尿阳性率为60%和40%，尿WBC阳性率为60%和40%，而庆大霉素对照组和鱼腥草0.25 g/mL阳性率仍为100%，均P＜0.01），制模9 d时两组均达到0水平，而0.25 g/mL组仍为20%的阳性率，说明鱼腥草0.50 g/mL和1.00 g/mL组抑菌作用较鱼腥草0.25 g/mL强。制模后各组WBC及N%均较制模前有所增加，3 d时已出现了统计学意义〔WBC（×109/L）：庆大霉素对照组为13.00±5.23比8.95±2.69，鱼腥草0.25 g/mL组为14.52±10.28比8.73±3.16，鱼腥草0.50 g/mL组为12.97±11.33比7.83±4.51，鱼腥草1.00 g/mL组为13.03±9.17比6.45±3.85；N%：庆大霉素对照组为（80.99±14.93比63.41±7.96，鱼腥草0.25 g/mL组为89.62±9.41比70.01±9.13，鱼腥草0.50 g/mL组为87.69±11.34比67.75±8.91，鱼腥草1.00 g/mL 组为85.67±12.81比65.12±4.63〕，7 d时有所下降，9 d时鱼腥草0.50 g/mL和1.00 g/mL组WBC和N%均恢复到正常范围〔WBC（×109/L）：鱼腥草0.50 g/mL组为8.56±5.38，鱼腥草1.00 g/mL组为7.93±4.82；N%：鱼腥草0.50 g/mL组为65.97±8.35，鱼腥草1.00 g/mL组为65.57±9.12〕。光镜下可见鱼腥草治疗组膀胱组织黏膜各层组织细胞形态正常，表明鱼腥草液对膀胱没有造成过敏等炎性反应。结论应用鱼腥草液进行膀胱冲洗治疗兔尿路感染，抑菌作用好于庆大霉素。
目的：觀察魚腥草液膀胱遲洗對兔膀胱感染模型的抑菌作用，為防治尿路感染提供新的方法。方法選擇雄性新西蘭長耳大白兔20隻，按隨機數字錶法分為慶大黴素對照組和3箇不同濃度魚腥草組，每組5隻。採用導尿管嚮兔膀胱註入大腸埃希菌液1 mL（105 cfu/mL）夾閉導尿管1 h的方法複製兔膀胱感染模型；魚腥草組分彆嚮膀胱註入0.25、0.50、1.00 g/mL魚腥草液50 mL，對照組嚮膀胱註入160 kU慶大黴素。于製模前及製模後3、5、7、9 d取兔耳血，檢測各組血白細胞計數（WBC）、中性粒細胞比例（N%）；同時取中段晨尿檢測菌尿暘性率和尿WBC暘性率；實驗結束後處死動物取膀胱組織，光鏡下觀察病理學改變。結果製模前各組動物菌尿和尿WBC均為陰性，血常規WBC、N%均在正常範圍。製模後3 d各組菌尿暘性率和尿WBC暘性率均達到100%；製模後隨著治療時間延長，各組菌尿暘性率和尿WBC暘性率逐漸下降，5 d時魚腥草0.50 g/mL和1.00 g/mL組已齣現統計學意義（菌尿暘性率為60%和40%，尿WBC暘性率為60%和40%，而慶大黴素對照組和魚腥草0.25 g/mL暘性率仍為100%，均P＜0.01），製模9 d時兩組均達到0水平，而0.25 g/mL組仍為20%的暘性率，說明魚腥草0.50 g/mL和1.00 g/mL組抑菌作用較魚腥草0.25 g/mL彊。製模後各組WBC及N%均較製模前有所增加，3 d時已齣現瞭統計學意義〔WBC（×109/L）：慶大黴素對照組為13.00±5.23比8.95±2.69，魚腥草0.25 g/mL組為14.52±10.28比8.73±3.16，魚腥草0.50 g/mL組為12.97±11.33比7.83±4.51，魚腥草1.00 g/mL組為13.03±9.17比6.45±3.85；N%：慶大黴素對照組為（80.99±14.93比63.41±7.96，魚腥草0.25 g/mL組為89.62±9.41比70.01±9.13，魚腥草0.50 g/mL組為87.69±11.34比67.75±8.91，魚腥草1.00 g/mL 組為85.67±12.81比65.12±4.63〕，7 d時有所下降，9 d時魚腥草0.50 g/mL和1.00 g/mL組WBC和N%均恢複到正常範圍〔WBC（×109/L）：魚腥草0.50 g/mL組為8.56±5.38，魚腥草1.00 g/mL組為7.93±4.82；N%：魚腥草0.50 g/mL組為65.97±8.35，魚腥草1.00 g/mL組為65.57±9.12〕。光鏡下可見魚腥草治療組膀胱組織黏膜各層組織細胞形態正常，錶明魚腥草液對膀胱沒有造成過敏等炎性反應。結論應用魚腥草液進行膀胱遲洗治療兔尿路感染，抑菌作用好于慶大黴素。
목적：관찰어성초액방광충세대토방광감염모형적억균작용，위방치뇨로감염제공신적방법。방법선택웅성신서란장이대백토20지，안수궤수자표법분위경대매소대조조화3개불동농도어성초조，매조5지。채용도뇨관향토방광주입대장애희균액1 mL（105 cfu/mL）협폐도뇨관1 h적방법복제토방광감염모형；어성초조분별향방광주입0.25、0.50、1.00 g/mL어성초액50 mL，대조조향방광주입160 kU경대매소。우제모전급제모후3、5、7、9 d취토이혈，검측각조혈백세포계수（WBC）、중성립세포비례（N%）；동시취중단신뇨검측균뇨양성솔화뇨WBC양성솔；실험결속후처사동물취방광조직，광경하관찰병이학개변。결과제모전각조동물균뇨화뇨WBC균위음성，혈상규WBC、N%균재정상범위。제모후3 d각조균뇨양성솔화뇨WBC양성솔균체도100%；제모후수착치료시간연장，각조균뇨양성솔화뇨WBC양성솔축점하강，5 d시어성초0.50 g/mL화1.00 g/mL조이출현통계학의의（균뇨양성솔위60%화40%，뇨WBC양성솔위60%화40%，이경대매소대조조화어성초0.25 g/mL양성솔잉위100%，균P＜0.01），제모9 d시량조균체도0수평，이0.25 g/mL조잉위20%적양성솔，설명어성초0.50 g/mL화1.00 g/mL조억균작용교어성초0.25 g/mL강。제모후각조WBC급N%균교제모전유소증가，3 d시이출현료통계학의의〔WBC（×109/L）：경대매소대조조위13.00±5.23비8.95±2.69，어성초0.25 g/mL조위14.52±10.28비8.73±3.16，어성초0.50 g/mL조위12.97±11.33비7.83±4.51，어성초1.00 g/mL조위13.03±9.17비6.45±3.85；N%：경대매소대조조위（80.99±14.93비63.41±7.96，어성초0.25 g/mL조위89.62±9.41비70.01±9.13，어성초0.50 g/mL조위87.69±11.34비67.75±8.91，어성초1.00 g/mL 조위85.67±12.81비65.12±4.63〕，7 d시유소하강，9 d시어성초0.50 g/mL화1.00 g/mL조WBC화N%균회복도정상범위〔WBC（×109/L）：어성초0.50 g/mL조위8.56±5.38，어성초1.00 g/mL조위7.93±4.82；N%：어성초0.50 g/mL조위65.97±8.35，어성초1.00 g/mL조위65.57±9.12〕。광경하가견어성초치료조방광조직점막각층조직세포형태정상，표명어성초액대방광몰유조성과민등염성반응。결론응용어성초액진행방광충세치료토뇨로감염，억균작용호우경대매소。
Objective To observe the bacteriostasis of herba houttuyniae liquid in bladder irrigation for treatment of rabbit bladder infection model, and to offer a new way for prevention and treatment of urinary tract infection.Methods Twenty male New Zealand long-eared white rabbits were divided into gentamicin control group and three different concentrations herba houttuyniae groups according to random number table method, each group 5 rabbits. The rabbit bladder infection model was replicated by injecting coli liquid 1 mL (105 cfu/mL) into the urinary bladder through a urethral catheter, and clipping the catheter for 1 hour. After model establishment for 3 days, in the three herba houttuyniae groups, 0.25, 0.50, 1.00 g/mL herba houttuyniae liquid 50 mL were injected into the bladder respectively, and in the gentamicin control group, 160 kU gentamicin was injected into the bladder. Before molding and after molding for 3, 5, 7, 9 days, ear blood in rabbit was taken, and white blood cell (WBC) count and neutrophil percentage (N%) were tested; at the same time, the middle segment morning urine was taken to detect bacteriuria and urine WBC positive rates; after the experiment, the animals were sacrificed, the urinary bladder tissues were taken, and the pathological changes in urinary bladder tissue were observed by a light microscope.Results Before molding, the bacteriuria in animals of each group was negative and the results of urine WBC, blood WBC and N% were within normal range. After molding for 3 days, bacteriuria and urine WBC positive rates reached 100%; with the prolongation of treatment after molding, the above two rates in each group were gradually decreased, and herba houttuyniae 0.50 g/mL and 1.00 g/mL groups appeared statistical significance on the 5th day (bacteriuria positive rate was 60% and 40%, urine WBC positive rate was 60% and 40% respectively, while the gentamicin control group and herba houttuyniae 0.25 g/mL group was still 100% positive, allP < 0.01); on the 9th day, the bacteriuria positive rate in both higher dosage herba houttuyniae groups was 0, in 0.25 g/mL group, it still had the positive rate 20%, showing herba houttuyniae 0.50 g/mL and 1.00 g/mL had stronger inhibitory effect than herba houttuyniae 0.25 g/mL. WBC and N% were increased in each group after molding, and on the 3rd day, WBC and N were significantly higher in each group than those before molding [WBC (×109/L): gentamicin control group 13.00±5.23 vs. 8.95±2.69, herba houttuyniae 0.25 g/mL group 14.52±10.28 vs. 8.73±3.16, herba houttuyniae 0.50 g/mL group 12.97±11.33 vs. 7.83±4.51, herba houttuyniae 1.00 g/mL group 13.03±9.17 vs. 6.45±3.85. N%: gentamicin control group 80.99±14.93 vs. 63.41±7.96, herba houttuyniae 0.25 g/mL group 89.62±9.41 vs. 70.01±9.13, herba houttuyniae 0.50 g/mL group 87.69±11.34 vs. 67.75±8.91, herba houttuyniae 1.00 g/mL group 85.67±12.81 vs. 65.12±4.63]. On the 7th day, WBC and N% fell, and on the 9th day, the WBC and N% were restored to normal range in herba houttuyniae 0.50 g/mL and 1.00 g/mL groups [WBC (×109/L): herba houttuyniae 0.50 g/mL group 8.56±5.38, herba houttuyniae 1.00 g/mL group 7.93±4.82. N%: herba houttuyniae 0.50 g/mL group 65.97±8.35, herba houttuyniae 1.00 g/mL group 65.57±9.12]. Under light microscopy, it was shown that the morphological appearances of tissue cells in various layers of bladder mucosa were normal in herba houttuyniae group, demonstrating that herba houttuyniae fluid in bladder did not cause allergic inflammatory reaction, etc.Conclusion The bacteriostatic action of herba houttuyniae liquid for bladder irrigation in treatment of rabbits with urinary tract infection is better than that of gentamicin.
