CAJ | 학술논문

目的：探索 TPD52基因在原发性肝细胞癌中的表达及与患者预后的关系。方法荧光定量 RT-PCR检测33对肝癌组织及其对应的癌旁组织中 TPD52 mRNA表达水平；免疫组化检测173例肝癌石蜡标本中 TPD52蛋白表达情况。结果肝癌组织中 TPD52 mRNA表达水平较癌旁组织低（P <0.001）；肝癌组织 TPD52蛋白表达水平低于癌旁组织，且其表达水平与 TNM分期存在负相关（P =0.023）；TPD52高表达的患者较低表达的患者预后好（P =0.003，P =0.013），且能明显延长肿瘤小于5 cm、肿瘤单发或 TNM分期为 III期的患者的总生存期（P =0.015，P =0.025，P =0.018）和无病进展生存期（P =0.023，P =0.047，P =0.034）。结论 TPD52低表达与原发性肝癌的发生发展有高度相关性，是肝癌的独立预后因素。
목적：탐색 TPD52기인재원발성간세포암중적표체급여환자예후적관계。방법형광정량 RT-PCR검측33대간암조직급기대응적암방조직중 TPD52 mRNA표체수평；면역조화검측173례간암석사표본중 TPD52단백표체정황。결과간암조직중 TPD52 mRNA표체수평교암방조직저（P <0.001）；간암조직 TPD52단백표체수평저우암방조직，차기표체수평여 TNM분기존재부상관（P =0.023）；TPD52고표체적환자교저표체적환자예후호（P =0.003，P =0.013），차능명현연장종류소우5 cm、종류단발혹 TNM분기위 III기적환자적총생존기（P =0.015，P =0.025，P =0.018）화무병진전생존기（P =0.023，P =0.047，P =0.034）。결론 TPD52저표체여원발성간암적발생발전유고도상관성，시간암적독립예후인소。
Objective To explore the gene of tumor protein D52 (TPD52) expression and its relationship with prognosis in patients with primary hepatocellular carcinoma (HCC). Methods Real-time quantitative PCR was performed in 33 paired clinical samples (tumor tissues and matched adjacent non-tumor liver tissues) from patients with HCC to determine their TPD52 mRNA levels. The TPD52 expression levels in 173 HCC surgical specimens were examined by immunohistochemistry. Results TPD52 mRNA expression was significantly lower in the tumor tissues than that in the matched adjacent non-tumor liver tissues (P < 0.001). In the surgical specimens, TPD52 protein expression was lower in tumor tissue than that in the paraneoplastic tissue, and its protein expression shows significantly negative correlation with the TNM stage of the tumor (P = 0.023). The overall survival and disease-free survival rates were significantly better in patients with high TPD52 expression when compared with those having low TPD52 expression (P = 0.003 andP = 0.013, respectively). In subgroup analysis, higher TPD52 expression indicated significantly better overall survival and disease-free survival in patients with tumor < 5cm, single tumor or III TNM stage (P = 0.015, P = 0.025, P = 0.018 andP = 0.023,P = 0.047, P = 0.034, respectively). Conclusion Lower TPD52 expression is highly correlated with the development and progression of HCC, and represents an independent prognostic factor for the survival of the patients. TPD52 could be a novel prognostic biomarker and molecular therapy target for HCC.