CAJ | 학술논문

目的：探讨建立南京医科大学第一附属医院生物样本库中血液生物样本 microRNA的检测方法以及血凝块中 DNA的鉴定方法。方法随机选取本院生物样本库自2012–2014年存储的胃癌、肝癌血浆标本进行 microRNA的提取，后经 Q-PCR验证 miR-21和 miR-387的表达情况以及将对应的血凝块样本进行全血样本 DNA的提取，通过全自动核酸分析仪鉴定提取的 DNA的完整性。结果 Q-PCR结果表明，冻存血浆在保存了1、2、3年后都能检测到相关的 microRNA的表达，同时血凝块中提取的 DNA，经全自动核酸分析仪鉴定，DNA完整性也较好。结论该方法能够初步验证我院生物样本库中冻存的血样标本中的 RNA和 DNA的保存质量，血样质量基本能满足后续科研实验的需求。本研究中的血样标本质量鉴定的方法切实、可行，能较准确地反映生物样本库中血样本的质量，为今后我院组织样本的质量控制体系的建立奠定基础。
목적：탐토건립남경의과대학제일부속의원생물양본고중혈액생물양본 microRNA적검측방법이급혈응괴중 DNA적감정방법。방법수궤선취본원생물양본고자2012–2014년존저적위암、간암혈장표본진행 microRNA적제취，후경 Q-PCR험증 miR-21화 miR-387적표체정황이급장대응적혈응괴양본진행전혈양본 DNA적제취，통과전자동핵산분석의감정제취적 DNA적완정성。결과 Q-PCR결과표명，동존혈장재보존료1、2、3년후도능검측도상관적 microRNA적표체，동시혈응괴중제취적 DNA，경전자동핵산분석의감정，DNA완정성야교호。결론해방법능구초보험증아원생물양본고중동존적혈양표본중적 RNA화 DNA적보존질량，혈양질량기본능만족후속과연실험적수구。본연구중적혈양표본질량감정적방법절실、가행，능교준학지반영생물양본고중혈양본적질량，위금후아원조직양본적질량공제체계적건립전정기출。
Objective To investigate methods for detecting DNA and microRNA in the blood samples of the biobank. Methods Samples of patients with gastric cancer and liver cancer were collected from year 2012 to 2014 in our hospital. Total miRNA was isolated from the plasma samples of the cancer patients. The specific expression of miR-387 and miR-21 were verified by Q-PCR. The DNA was extracted from whole blood samples and the DNA integrity was also verified by the automatic nucleic acid analyzer. Results Q-PCR results showed that the expression of microRNA was detected in all frozen plasma samples kept for 1, 2, or 3 years. The data of DNA extraction from blood clots, identified by the automated nucleic acid analyzer, demonstrated that the DNA integrity was better. Conclusions In our study, this method can be used to verify the quality of DNA and microRNA of blood samples in our hospital. After detection, the quality of blood samples is able to fit the needs of the follow-up research. The method for identification of the quality of blood samples in the study is practical and feasible and the results can accurately reflect the quality of blood samples in the biobank, which lays the foundation for the establishment of quality control system in our hospital in future.