CAJ | 학술논문

小麦6-SFT是果聚糖合成的关键酶基因。以23份六倍体普通小麦(AABBDD)、5份D基因组材料(DD)为多样性代表群体材料,通过测序分析小麦6-SFT-D基因的序列多态性,根据多态性开发6-SFT-D基因的功能标记,分析由154份六倍体普通小麦构成的自然群体的6-SFT-D基因单倍型(haplotype)与表型性状的关联特性和基因累加效应。在28份多样性代表群体中,共检测到6-SFT-D 基因的4个多态性位点,均为单核苷酸多态性(SNP)位点,构成3种6-SFT-D基因单倍型；而在自然群体中只检测到6-SFT-D的两种单倍型。根据6-SFT-D基因2850 bp位点的T/C变异开发等位变异特异 PCR 标记。关联分析表明,6-SFT-D 单倍型分别与灌溉条件下的千粒重和穗长显著关联,单倍型Hap I是提高千粒重的优异等位变异；在雨养和灌溉条件下,同时具有6-SFT-D与6-SFT-A2优异等位变异小麦材料的千粒重显著高于其他基因型材料,说明6-SFT-D和6-SFT-A2优异等位变异对于提高千粒重表现累加效应。
소맥6-SFT시과취당합성적관건매기인。이23빈륙배체보통소맥(AABBDD)、5빈D기인조재료(DD)위다양성대표군체재료,통과측서분석소맥6-SFT-D기인적서렬다태성,근거다태성개발6-SFT-D기인적공능표기,분석유154빈륙배체보통소맥구성적자연군체적6-SFT-D기인단배형(haplotype)여표형성상적관련특성화기인루가효응。재28빈다양성대표군체중,공검측도6-SFT-D 기인적4개다태성위점,균위단핵감산다태성(SNP)위점,구성3충6-SFT-D기인단배형；이재자연군체중지검측도6-SFT-D적량충단배형。근거6-SFT-D기인2850 bp위점적T/C변이개발등위변이특이 PCR 표기。관련분석표명,6-SFT-D 단배형분별여관개조건하적천립중화수장현저관련,단배형Hap I시제고천립중적우이등위변이；재우양화관개조건하,동시구유6-SFT-D여6-SFT-A2우이등위변이소맥재료적천립중현저고우기타기인형재료,설명6-SFT-D화6-SFT-A2우이등위변이대우제고천립중표현루가효응。
Gene 6-SFT encodes a key enzyme in fructan biosynthesis pathway in common wheat (Triticum aestivum L.). In this study, we analyzed the single nucleotide polymorphism (SNP) on 6-SFT-D locus in a diversity population of 23 hexaploid wheat (AABBDD) accessions and five wheat relative species (DD) by means of direct sequencing. Functional markers were developed according to the sequence polymorphism. The correlation between 6-SFT-D haplotypes and phenotypic traits and the cumulative effect of 6-SFT alleles were analyzed using a natural population consisting of 154 historical wheat accessions. Four SNPs were detected in 6-SFT-D sequences in the diversity population, forming three haplotypes. However, only two 6-SFT-D haplotypes were identified in the natural population. We developed a pair of allele-specific PCR markers based on a polymorphism (T/C) at the 2850 bp site. The results of haplotype–trait association analysis showed that 6-SFT-D was significantly associated with thou-sand-grain weight (TGW) and spike length under well-watered conditions. Hap I was a superior allele in improving TGW. Under drought stress and well-watered conditions, wheat materials carrying both superior allele of 6-SFT-D and 6-SFT-A2 had signifi-cantly higher TGW than other genotypes, suggesting that 6-SFT-D and 6-SFT-A2 have cumulative effect on TGW improvement.