CAJ | 학술논문

结合分子标记辅助轮回选择和田间鉴定的方法，将三黄占2号的抗稻瘟病基因Pi-GD-1(t)和Pi-GD-2(t)(分别简称G1和G2)、CBB23中的抗白叶枯病基因Xa23(简称X)和IR65482-7-216-1-2-B (简称IR65482)的抗褐飞虱基因Bph18(t)(简称B)导入温恢845、温恢117和温恢143等3个中籼恢复系，获得了8个兼抗稻瘟病和褐飞虱聚合系，温恢845-G1-G2-B-4、温恢845-G1-G2-B-5、温恢117-G1-G2-X-B-3、温恢143-G1-G2-B-3、温恢143-G2-X-B-9、温恢143-G2-X-B-10、温恢143-G1-G2-B-11和温恢143-G1-G2-B-37。这些聚合系及其与不育系五丰A的测交种，对稻瘟病和褐飞虱的抗性水平接近或略低于稻瘟病抗性亲本三黄占2号和稻飞虱抗性亲本 IR65482。部分改良恢复系如温恢117-G1-G2-X-B-3、温恢143-G2-X-B-9和温恢143-G2-X-B-10及其测交种对白叶枯病表现为抗病或中抗。改良恢复系及其测交种在正常条件下的农艺性状与原始恢复系及其测交种相仿或更优，具有生产应用价值。研究结果表明， Xa23在不同恢复系背景下抗性表达完全，而Pi-GD-1(t)、Pi-GD-2(t)和Bph18(t)对稻瘟病和褐飞虱抗性的改良效果与恢复系的遗传背景有关。
결합분자표기보조륜회선택화전간감정적방법，장삼황점2호적항도온병기인Pi-GD-1(t)화Pi-GD-2(t)(분별간칭G1화G2)、CBB23중적항백협고병기인Xa23(간칭X)화IR65482-7-216-1-2-B (간칭IR65482)적항갈비슬기인Bph18(t)(간칭B)도입온회845、온회117화온회143등3개중선회복계，획득료8개겸항도온병화갈비슬취합계，온회845-G1-G2-B-4、온회845-G1-G2-B-5、온회117-G1-G2-X-B-3、온회143-G1-G2-B-3、온회143-G2-X-B-9、온회143-G2-X-B-10、온회143-G1-G2-B-11화온회143-G1-G2-B-37。저사취합계급기여불육계오봉A적측교충，대도온병화갈비슬적항성수평접근혹략저우도온병항성친본삼황점2호화도비슬항성친본 IR65482。부분개량회복계여온회117-G1-G2-X-B-3、온회143-G2-X-B-9화온회143-G2-X-B-10급기측교충대백협고병표현위항병혹중항。개량회복계급기측교충재정상조건하적농예성상여원시회복계급기측교충상방혹경우，구유생산응용개치。연구결과표명， Xa23재불동회복계배경하항성표체완전，이Pi-GD-1(t)、Pi-GD-2(t)화Bph18(t)대도온병화갈비슬항성적개량효과여회복계적유전배경유관。
The novel blast resistance genes, Pi-GD-1(t) and Pi-GD-2(t) (abbreviated as G1 and G2, respectively) from rice culti-var Sanhuangzhan2, bacterial blight resistance gene Xa23 (abbreviated as X) from CBB23 and brown planthopper resistance gene Bph18(t) (abbreviated as B) from IR65482-7-216-1-2-B (abbreviated as IR65482) were introgressed into three rice restorer lines, Wenhui 845, Wenhui 117 and Wenhui 143, by marker-assisted backcross and pyramiding breeding methods integrated with artifi-cial inoculation, natural induction and phenotypic selections. The eight resistant restorer lines pyramiding blast and brown planthopper resistance genes, i.e. Wenhui 845-G1-G2-B-4, Wenhui 845-G1-G2-B-5, Wenhui 117-G1-G2-X-B-3, Wenhui 143-G1-G2-B-3, Wenhui 143-G2-X-B-9, Wenhui 143-G2-X-B-10, Wenhui 143-G1-G2-B-11, and Wenhui 143-G1-G2-B-37, and their tester lines crossed with the sterile line Wufeng A demonstrated the similar or slight lower level of resistance against rice blast and brown planthopper as compared with the resistant donor parents Sanhuangzhan 2 or IR65482. Part of developed restorer lines (Wenhui 117-G1-G2-X-B-3, Wenhui 143-G2-X-B-9, and Wenhui 143-G2-X-B-10) and their tester lines showed resistance or moderate resistance to bacterial blight. The newly developed restorers resistant to blast, bacterial blight and brown planthopper were similar or superior to their respective original types in agronomic traits under normal condition, implying these resistant restorer lines can be useful in hybrid rice breeding and production. The results indicated that Xa23 could completely express its dominant resistance against different restorer genetic backgrounds whereas effects of resistance improvement in Pi-GD-1(t), Pi-GD-2(t), and Bph18(t) depend on their genetic background.