湖南工业大学学报
湖南工業大學學報
호남공업대학학보
Journal of Hunan University of Technology
2015年
5期
96-101
,共6页
刘丽莉%白宁宁%谢婧%严明理
劉麗莉%白寧寧%謝婧%嚴明理
류려리%백저저%사청%엄명리
稻米%原花色素%显色反应%DMACA染色法%铁铵矾染色法%香草醛染色法
稻米%原花色素%顯色反應%DMACA染色法%鐵銨礬染色法%香草醛染色法
도미%원화색소%현색반응%DMACA염색법%철안반염색법%향초철염색법
rice%proanthocyanidins%color reaction%DMACA staining%ferric ammonium alum staining%vanillin staining
利用二甲基乙酰胺二甲基缩醛（DMACA）染色法、铁铵矾染色法和香草醛染色法，测定了不同品种稻米籽粒中原花色素的含量。用DMACA染色法测得谭农s/制21、R288、凡-14、12庚351、秦稻2号籽粒中原花色素的质量分数依次为0.136,0.183,0.085,4.115,24.736 mg/g；用铁铵矾染色法测得的质量分数依次为0.292,0.094,1.940×10-4,1.220,8.080 mg/g；用香草醛染色法测得的质量分数依次为1.349,1.363,1.093,3.733,15.153 mg/g。检测结果表明：5种稻米品种中，秦稻2号的原花色素含量最高，是12庚351的4.1~6.6倍，凡-14中的含量最低；黑米品种（秦稻2号和12庚351）比白米品种（R288、谭农s/制21和凡-14）中原花色素的含量明显要高。吸光度相对标准偏差（RSD）值表明，3种染色法测定原花色素含量的精密度由高到低依次为：香草醛染色法、DMACA染色法、铁铵矾染色法。
利用二甲基乙酰胺二甲基縮醛（DMACA）染色法、鐵銨礬染色法和香草醛染色法，測定瞭不同品種稻米籽粒中原花色素的含量。用DMACA染色法測得譚農s/製21、R288、凡-14、12庚351、秦稻2號籽粒中原花色素的質量分數依次為0.136,0.183,0.085,4.115,24.736 mg/g；用鐵銨礬染色法測得的質量分數依次為0.292,0.094,1.940×10-4,1.220,8.080 mg/g；用香草醛染色法測得的質量分數依次為1.349,1.363,1.093,3.733,15.153 mg/g。檢測結果錶明：5種稻米品種中，秦稻2號的原花色素含量最高，是12庚351的4.1~6.6倍，凡-14中的含量最低；黑米品種（秦稻2號和12庚351）比白米品種（R288、譚農s/製21和凡-14）中原花色素的含量明顯要高。吸光度相對標準偏差（RSD）值錶明，3種染色法測定原花色素含量的精密度由高到低依次為：香草醛染色法、DMACA染色法、鐵銨礬染色法。
이용이갑기을선알이갑기축철（DMACA）염색법、철안반염색법화향초철염색법，측정료불동품충도미자립중원화색소적함량。용DMACA염색법측득담농s/제21、R288、범-14、12경351、진도2호자립중원화색소적질량분수의차위0.136,0.183,0.085,4.115,24.736 mg/g；용철안반염색법측득적질량분수의차위0.292,0.094,1.940×10-4,1.220,8.080 mg/g；용향초철염색법측득적질량분수의차위1.349,1.363,1.093,3.733,15.153 mg/g。검측결과표명：5충도미품충중，진도2호적원화색소함량최고，시12경351적4.1~6.6배，범-14중적함량최저；흑미품충（진도2호화12경351）비백미품충（R288、담농s/제21화범-14）중원화색소적함량명현요고。흡광도상대표준편차（RSD）치표명，3충염색법측정원화색소함량적정밀도유고도저의차위：향초철염색법、DMACA염색법、철안반염색법。
The proanthocyanidins content was determined in rice grain of different colors using dimethylamino-cinnamaldehyde (DMACA) staining, Ferric ammonium alum staining and Vanillin staining. Experimental studies showed that the proanthocyanidins mass fraction of five rice breeds (Tannong s/zhi21, R288, Fan-14, 12Geng351 and Qingdao2) detected by DMACA staining was successively as follows, 0.136, 0.183, 0.085, 4.115 and 24.736 mg/g (dry weight); the result detected by Ferric ammonium alum staining was 0.292, 0.094, 1.940×10-4, 1.220 and 8.080 mg/g; and the result detected by Vanillin-HCl staining was 1.349, 1.363, 1.093, 3.733 and 15.153 mg/g. The proanthocyanidins content of Qingdao2 was the highest, which was about 4.1 to 6.6 times of 12Geng351, and Fan-14 was the lowest in five rice breeds; the proanthocyanidins content of black rice breeds (Qingdao2 and 12Geng351) was higher than that of white rice breeds (Tannong s/zhi21, R288, Fan-14). The relative standard deviation (RSD) of the absorbency showed that the precision level of determining proanthocyanidins content as follow, the first was vanillin staining, the second was DMACA staining and the third was ferric ammonium alum staining.
