山西中医学院学报
山西中醫學院學報
산서중의학원학보
Journal of Shanxi College of Traditional Chinese Medicine
2015年
5期
16-17
,共2页
林翔%王荣茂%聂光瑞%张俐
林翔%王榮茂%聶光瑞%張俐
림상%왕영무%섭광서%장리
缺血再灌注%脊髓神经细胞%动物模型
缺血再灌註%脊髓神經細胞%動物模型
결혈재관주%척수신경세포%동물모형
ischemia-reperfusion%spinal cord nerve cells%animal model
目的：探索SD胎鼠原代脊髓神经细胞缺血再灌注损伤模型的建立方法。方法：将妊娠15 d的孕鼠处死后取出胎鼠脊髓，分离神经细胞行原代细胞培养，采用谷氨酸诱导脊髓神经损伤的方法制作脊髓缺血再灌注损伤模型，通过测定细胞活力及培养液中LDH、MDA含量确定谷氨酸的最佳浓度及作用时间，鉴定模型。结果：采用200μmol/mL谷氨酸液作用15 min可方便地制作脊髓神经细胞缺血再灌注损伤模型。结论：通过模拟谷氨酸过度释放环节，制作脊髓神经细胞缺血再灌注损伤模型，方法简便，可重复性高。
目的：探索SD胎鼠原代脊髓神經細胞缺血再灌註損傷模型的建立方法。方法：將妊娠15 d的孕鼠處死後取齣胎鼠脊髓，分離神經細胞行原代細胞培養，採用穀氨痠誘導脊髓神經損傷的方法製作脊髓缺血再灌註損傷模型，通過測定細胞活力及培養液中LDH、MDA含量確定穀氨痠的最佳濃度及作用時間，鑒定模型。結果：採用200μmol/mL穀氨痠液作用15 min可方便地製作脊髓神經細胞缺血再灌註損傷模型。結論：通過模擬穀氨痠過度釋放環節，製作脊髓神經細胞缺血再灌註損傷模型，方法簡便，可重複性高。
목적：탐색SD태서원대척수신경세포결혈재관주손상모형적건립방법。방법：장임신15 d적잉서처사후취출태서척수，분리신경세포행원대세포배양，채용곡안산유도척수신경손상적방법제작척수결혈재관주손상모형，통과측정세포활력급배양액중LDH、MDA함량학정곡안산적최가농도급작용시간，감정모형。결과：채용200μmol/mL곡안산액작용15 min가방편지제작척수신경세포결혈재관주손상모형。결론：통과모의곡안산과도석방배절，제작척수신경세포결혈재관주손상모형，방법간편，가중복성고。
Objective:To establish animal model of spinal cord nerve cells ischemia-reperfusion in rats. Methods:The rats on 15th day of gestation were killed,spinal cord nerve cells of fetal rat was taken out. Then nerve cells were separated and cultured. The spinal nerve cells ischemia-reperfusion model was established by damaging induced by glutamate. The ap-propriate concentration and duration of glutamate were determined by cell viability,content of LDH and MDA in culture medium. Results:It was easy to establish the spinal nerve cell ischemia-reperfusion model by exposed to 200 μmol/mL glutamate for 15 min. Conclusion:This method is convenient and repeatable.
