中华诊断学电子杂志
中華診斷學電子雜誌
중화진단학전자잡지
Chinese Journal of Diagnostics (Electronic Edition)
2015年
4期
296-299
,共4页
细菌%RNA,核糖体,16S%聚合酶链反应
細菌%RNA,覈糖體,16S%聚閤酶鏈反應
세균%RNA,핵당체,16S%취합매련반응
Bacteria%RNA,16S,ribosomal%Polymerase chain reaction
目的：探讨通过16S核糖体RNA（16S rRNA ）基因测序的方法用于临床菌株鉴定。方法以细菌16S rRNA基因为靶序列，采用细菌的通用引物P1、P2进行PCR扩增，同时送到测序公司测序从而达到鉴定。结果待测菌株及ATCC25923阳性对照通过PCR得到的扩增片段为1465bp左右，待测菌株经测序比对鉴定为一株杰氏棒状杆菌，阳性质控ATCC25923鉴定为一株金黄的葡萄球菌，阴性对照未出现任何结果。结论通过PCR检测细菌16srRNA基因可以应用于临床菌株的鉴定，并且适用一些难以鉴定的细菌。
目的：探討通過16S覈糖體RNA（16S rRNA ）基因測序的方法用于臨床菌株鑒定。方法以細菌16S rRNA基因為靶序列，採用細菌的通用引物P1、P2進行PCR擴增，同時送到測序公司測序從而達到鑒定。結果待測菌株及ATCC25923暘性對照通過PCR得到的擴增片段為1465bp左右，待測菌株經測序比對鑒定為一株傑氏棒狀桿菌，暘性質控ATCC25923鑒定為一株金黃的葡萄毬菌，陰性對照未齣現任何結果。結論通過PCR檢測細菌16srRNA基因可以應用于臨床菌株的鑒定，併且適用一些難以鑒定的細菌。
목적：탐토통과16S핵당체RNA（16S rRNA ）기인측서적방법용우림상균주감정。방법이세균16S rRNA기인위파서렬，채용세균적통용인물P1、P2진행PCR확증，동시송도측서공사측서종이체도감정。결과대측균주급ATCC25923양성대조통과PCR득도적확증편단위1465bp좌우，대측균주경측서비대감정위일주걸씨봉상간균，양성질공ATCC25923감정위일주금황적포도구균，음성대조미출현임하결과。결론통과PCR검측세균16srRNA기인가이응용우림상균주적감정，병차괄용일사난이감정적세균。
Objective To detect clinical strain by amplifying 16S ribosomal RNA( 16S rRNA) gene with polymerase chain reaction ( PCR) method. Methods 16S rRNA gene was amplifyied by universal primers P1,P2 with polymerase chain reaction,and the polymerase chain reaction products were sequenced to achieve the DNA sequences. Results PCR products were about 1 465bp, and the PCR sequences were blasted in NCBI web to achieve the proper strain name,with the use of this method,the unkown gram?positive bacilli strain was identified as corynebacterium jeikeium strain, ATCC25923 was identified as a strain of staphylococcus aureus and negative control was also specific.Conclusion 16S rRNA gene detection can be used as strain identification,and especially for some less common clinical strains.
