CAJ | 학술논문

目的：探讨一种用图像分析软件Image-Pro Plus（IPP）联合图像处理软件Photoshop定量测定海马组织微循环改变的新方法。方法选择双侧颈总动脉结扎2周后无神经功能障碍的日本大耳白兔22只，按随机数字表法分为蛛网膜下腔出血（SAH）组和对照组，每组11只。采用枕大池二次注血法建立兔症状性脑血管痉挛模型；于第1次注血后7 d处死动物，进行大脑灌注固定，取兔海马区组织，用免疫组化法测定CD34。利用IPP 6.0联合Photoshop软件，定量测定海马组织中微血管密度（MVD）计数和统计场微血管面积比。结果 CD34可以有效标识微血管内皮细胞；用IPP 6.0可自动、准确地计算出海马区MVD和统计场微血管面积比。与对照组比较，SAH组兔海马区MVD计数与面积比均明显减少，差异均有统计学意义〔MVD（个/统计场）为3.87±0.67比5.17±0.53，统计场微血管面积比为（0.86±0.20）%比（1.40±0.17）%，均P＜0.05〕。结论 CD34可以用于标识微血管。IPP 6.0联合Photoshop图像分析软件是一种高效、准确统计组织中微血管数量与面积的工具，不仅操作简便，而且数据计算自动生成，可以准确反映兔症状性脑血管痉挛后海马区微循环改变。
목적：탐토일충용도상분석연건Image-Pro Plus（IPP）연합도상처리연건Photoshop정량측정해마조직미순배개변적신방법。방법선택쌍측경총동맥결찰2주후무신경공능장애적일본대이백토22지，안수궤수자표법분위주망막하강출혈（SAH）조화대조조，매조11지。채용침대지이차주혈법건립토증상성뇌혈관경련모형；우제1차주혈후7 d처사동물，진행대뇌관주고정，취토해마구조직，용면역조화법측정CD34。이용IPP 6.0연합Photoshop연건，정량측정해마조직중미혈관밀도（MVD）계수화통계장미혈관면적비。결과 CD34가이유효표식미혈관내피세포；용IPP 6.0가자동、준학지계산출해마구MVD화통계장미혈관면적비。여대조조비교，SAH조토해마구MVD계수여면적비균명현감소，차이균유통계학의의〔MVD（개/통계장）위3.87±0.67비5.17±0.53，통계장미혈관면적비위（0.86±0.20）%비（1.40±0.17）%，균P＜0.05〕。결론 CD34가이용우표식미혈관。IPP 6.0연합Photoshop도상분석연건시일충고효、준학통계조직중미혈관수량여면적적공구，불부조작간편，이차수거계산자동생성，가이준학반영토증상성뇌혈관경련후해마구미순배개변。
Objective To investigate a new method using Image-Pro Plus (IPP) combined with Photoshop image analysis software to quantitatively measure the changes in microcirculation in hippocampus.Methods Twenty-two Japanese white rabbits that had received bilateral carotid artery ligation for 2 weeks without neurological dysfunction were divided into a subarachnoid hemorrhage (SAH) group and a control group, 11 rabbits in each group. The rabbit model of symptomatic cerebral vasospasm was established by the method of twice injecting blood into occipital cistern. On the 7th day after the first time of injecting blood, the rabbits were sacrificed, cerebral perfusion fixation was carried out, the hippocampus was harvested, and CD34 was determined by immunohistochemical determination. IPP 6.0 combined with Photoshop image analysis software was used to quantitatively measure the count of hippocampal microvessels density (MVD) and the field (for statistics)/microvascular capillary area ratio was calculated.Results CD34 could effectively identify microvascular endothelial cells, and using IPP 6.0 could automatically and accurately calculate MVD and field (for statistics)/micrevascular area ratio in hippocampus. Compared with the control group, in SAH group, the MVD and the area ratio in hippocampus were significantly reduced, the differences being statistically significant [MVD (count/area): 3.87±0.67 vs. 5.17±0.53, area ratio: (0.86±0.20)% vs. (1.40±0.17)%, bothP < 0.05].Conclusions CD34 can be used to identify microvessels, IPP 6.0 image analysis software co-Photoshop is a high efficient and accurate new method to measure the microvessels count and calculate the field/microvessel area ratio, not only it is easy to operate, but also the data can be automatically calculated and generated, reflecting precisely the changes in microcirculation in hippocampus after symptomatic cerebral vasospasm in rabbits.