中国药业
中國藥業
중국약업
China Pharmaceuticals
2015年
21期
119-121
,共3页
高效液相色谱法%氯胺酮%咪唑安定%含量测定
高效液相色譜法%氯胺酮%咪唑安定%含量測定
고효액상색보법%록알동%미서안정%함량측정
HPLC%ketamine%midazolam%content determination
目的 建立同时测定镇痛凝胶剂中氯胺酮和咪唑安定含量的高效液相色谱( HPLC )法.方法 采用RP-HPLC外标法,色谱柱为Hypersil C18柱(250 mm × 4. 6 mm,5μg),流动相为乙腈-甲醇-0. 01%三乙胺(33:45:22,V: V: V),流速为0. 8 mL/min,柱温为25℃,检测波长为220 nm.结果 氯胺酮和咪唑安定的进样质量浓度线性范围分别为24. 32~243. 2μg/mL( r=0. 999 2)和20. 28~202. 8μg/mL ( r=0. 999 6),日内及日间精密度的 RSD均小于10%,平均回收率分别为97. 78%和100. 49%.结论 该方法快捷方便、结果可靠,可作为镇痛凝胶剂的质量控制方法.
目的 建立同時測定鎮痛凝膠劑中氯胺酮和咪唑安定含量的高效液相色譜( HPLC )法.方法 採用RP-HPLC外標法,色譜柱為Hypersil C18柱(250 mm × 4. 6 mm,5μg),流動相為乙腈-甲醇-0. 01%三乙胺(33:45:22,V: V: V),流速為0. 8 mL/min,柱溫為25℃,檢測波長為220 nm.結果 氯胺酮和咪唑安定的進樣質量濃度線性範圍分彆為24. 32~243. 2μg/mL( r=0. 999 2)和20. 28~202. 8μg/mL ( r=0. 999 6),日內及日間精密度的 RSD均小于10%,平均迴收率分彆為97. 78%和100. 49%.結論 該方法快捷方便、結果可靠,可作為鎮痛凝膠劑的質量控製方法.
목적 건립동시측정진통응효제중록알동화미서안정함량적고효액상색보( HPLC )법.방법 채용RP-HPLC외표법,색보주위Hypersil C18주(250 mm × 4. 6 mm,5μg),류동상위을정-갑순-0. 01%삼을알(33:45:22,V: V: V),류속위0. 8 mL/min,주온위25℃,검측파장위220 nm.결과 록알동화미서안정적진양질량농도선성범위분별위24. 32~243. 2μg/mL( r=0. 999 2)화20. 28~202. 8μg/mL ( r=0. 999 6),일내급일간정밀도적 RSD균소우10%,평균회수솔분별위97. 78%화100. 49%.결론 해방법쾌첩방편、결과가고,가작위진통응효제적질량공제방법.
Objective To establish an HPLC method to simultaneously determine ketamine and midazolam in Zhengtong Gels. Methods The separation was carried out on a Hypersil C18 column ( 250 mm × 4. 6 mm,5 μm ) with a mobile phase of acetonitrile-methanol-0. 01% triethylamine(33:45:22, V: V: V). The detection wavelength was 220 nm, the flow rate was 0. 8 mL/min and the column temperature was 25 ℃. Results The linear range of ketamine and midazolam were 24. 32-243. 2 μg/mL ( r=0. 999 2 ) , 20. 28-202. 8 μg/mL ( r=0. 999 6 ) , respectively. The inter-day and intra-day precision( RSD)were both less than 10%. The average recovery ( n=9 ) were 97. 78% and 100. 49%, respectively. Conclusion The method is simple, rapid, sensitive and reproducible.
