中国药业
中國藥業
중국약업
China Pharmaceuticals
2015年
21期
115-117
,共3页
申丽莎%陈国庆%杨帆%邓开英
申麗莎%陳國慶%楊帆%鄧開英
신려사%진국경%양범%산개영
吴茱萸%吴茱萸内酯%吴茱萸碱%吴茱萸次碱%高效液相色谱法%含量测定
吳茱萸%吳茱萸內酯%吳茱萸堿%吳茱萸次堿%高效液相色譜法%含量測定
오수유%오수유내지%오수유감%오수유차감%고효액상색보법%함량측정
Euodiae Fructus%Evodine%Evodiamine%Rutaecarpine%HPLC%wavelength switching%determination
目的 建立同时测定吴茱萸中吴茱萸内酯、吴茱碱和吴茱萸次碱含量的高效液相色谱(HPLC)波长切换法.方法 色谱柱为C18柱(250 mm × 4. 6 mm,5μm),流动相为乙腈-0. 2%磷酸溶液(35:65),流速为2. 0 mL/min,柱温为30℃,波长切换(0~15 min,205 nm;15. 01~28 min,225 nm).结果 吴茱萸内酯、吴茱萸碱和吴茱萸次碱进样量分别在0. 186~3. 72μg,0. 040 24~0. 804 8μg,0. 040 08~0. 801 6μg范围内与峰面积线性关系良好( r=0. 999 7,0. 999 9,0. 999 9 ) ,平均回收率分别为96. 30%,97. 66%,97. 46%,RSD分别为1. 43%,1. 32%,1. 80%( n=6).结论 该法简便,快速准确,灵敏度高,重复性好,可用于吴茱萸药材的质量控制.
目的 建立同時測定吳茱萸中吳茱萸內酯、吳茱堿和吳茱萸次堿含量的高效液相色譜(HPLC)波長切換法.方法 色譜柱為C18柱(250 mm × 4. 6 mm,5μm),流動相為乙腈-0. 2%燐痠溶液(35:65),流速為2. 0 mL/min,柱溫為30℃,波長切換(0~15 min,205 nm;15. 01~28 min,225 nm).結果 吳茱萸內酯、吳茱萸堿和吳茱萸次堿進樣量分彆在0. 186~3. 72μg,0. 040 24~0. 804 8μg,0. 040 08~0. 801 6μg範圍內與峰麵積線性關繫良好( r=0. 999 7,0. 999 9,0. 999 9 ) ,平均迴收率分彆為96. 30%,97. 66%,97. 46%,RSD分彆為1. 43%,1. 32%,1. 80%( n=6).結論 該法簡便,快速準確,靈敏度高,重複性好,可用于吳茱萸藥材的質量控製.
목적 건립동시측정오수유중오수유내지、오수감화오수유차감함량적고효액상색보(HPLC)파장절환법.방법 색보주위C18주(250 mm × 4. 6 mm,5μm),류동상위을정-0. 2%린산용액(35:65),류속위2. 0 mL/min,주온위30℃,파장절환(0~15 min,205 nm;15. 01~28 min,225 nm).결과 오수유내지、오수유감화오수유차감진양량분별재0. 186~3. 72μg,0. 040 24~0. 804 8μg,0. 040 08~0. 801 6μg범위내여봉면적선성관계량호( r=0. 999 7,0. 999 9,0. 999 9 ) ,평균회수솔분별위96. 30%,97. 66%,97. 46%,RSD분별위1. 43%,1. 32%,1. 80%( n=6).결론 해법간편,쾌속준학,령민도고,중복성호,가용우오수유약재적질량공제.
Objective To develop an HPLC method for simultaneous determination of 3 components in Euodiae Fructus. Methods A C18 column ( 250 mm × 4. 6 mm, 5 μm ) was used with the mobile phase of acetonitrile-0. 2% phosphoric acid ( 35:65 ) with the flow rate of 2. 0 mL/min and the column temperature was 30 ℃. The detection wavelength was set at 205 nm for Evodine in the first 15. 01 min, and then changed to 225 nm for evodiamine and rutaecarpine between 15. 01 and 28 min. Results The linear ranges of evodine, evo-diamine and rutaecarpine fell within the ranges of 0. 186~3. 72 μg, 0. 040 24~0. 804 8 μg, 0. 040 08~0. 801 6 μg, respectively ( r=0. 999 7,0. 999 9,0. 999 9 ) . The average recoveries were 96. 30%, 97. 66% and 97. 46% with RSD were 1. 43%, 1. 32% and 1. 80%, respectively( n=6). Conclusion The method is convenient,quick,accurate and suitable for the qulity control of Euodiae Fructus.
