中国药业
中國藥業
중국약업
China Pharmaceuticals
2015年
21期
121-123
,共3页
反相高效液相色谱法%正柴胡饮合剂%橙皮苷%芍药苷
反相高效液相色譜法%正柴鬍飲閤劑%橙皮苷%芍藥苷
반상고효액상색보법%정시호음합제%등피감%작약감
RP-HPLC method%Radix Bupleuri Decoction%hesperidin%paeoniflorin
目的 建立同时测定正柴胡饮合剂中橙皮苷和芍药苷含量的反相高效液相色谱(RP-HPLC)法.方法 采用超声提取的方法,色谱柱为phenomenex-ODS柱( 250 mm × 4. 60 mm,5μm ) ,柱温为30~35℃,流动相为乙腈-1 mol/L磷酸溶液(15:85),橙皮苷和芍药苷的检测波长分别为283 nm和230 nm,流速为0. 8~1. 0 mL/min.结果 橙皮苷进样量在0. 021 99~4. 420μg( r=0. 999 9)范围内与峰面积线性关系良好,平均回收率为95. 00%,RSD为4. 64%;芍药进样量在0. 201 0 ~1. 002 3μg( r=0. 999 9)范围内与峰面积线性关系良好,平均回收率为98. 04%,RSD为3. 60%.结论 该法操作简便,检测灵敏,结果准确,对于正柴胡饮合剂的质量控制具有非常重要的作用和意义.
目的 建立同時測定正柴鬍飲閤劑中橙皮苷和芍藥苷含量的反相高效液相色譜(RP-HPLC)法.方法 採用超聲提取的方法,色譜柱為phenomenex-ODS柱( 250 mm × 4. 60 mm,5μm ) ,柱溫為30~35℃,流動相為乙腈-1 mol/L燐痠溶液(15:85),橙皮苷和芍藥苷的檢測波長分彆為283 nm和230 nm,流速為0. 8~1. 0 mL/min.結果 橙皮苷進樣量在0. 021 99~4. 420μg( r=0. 999 9)範圍內與峰麵積線性關繫良好,平均迴收率為95. 00%,RSD為4. 64%;芍藥進樣量在0. 201 0 ~1. 002 3μg( r=0. 999 9)範圍內與峰麵積線性關繫良好,平均迴收率為98. 04%,RSD為3. 60%.結論 該法操作簡便,檢測靈敏,結果準確,對于正柴鬍飲閤劑的質量控製具有非常重要的作用和意義.
목적 건립동시측정정시호음합제중등피감화작약감함량적반상고효액상색보(RP-HPLC)법.방법 채용초성제취적방법,색보주위phenomenex-ODS주( 250 mm × 4. 60 mm,5μm ) ,주온위30~35℃,류동상위을정-1 mol/L린산용액(15:85),등피감화작약감적검측파장분별위283 nm화230 nm,류속위0. 8~1. 0 mL/min.결과 등피감진양량재0. 021 99~4. 420μg( r=0. 999 9)범위내여봉면적선성관계량호,평균회수솔위95. 00%,RSD위4. 64%;작약진양량재0. 201 0 ~1. 002 3μg( r=0. 999 9)범위내여봉면적선성관계량호,평균회수솔위98. 04%,RSD위3. 60%.결론 해법조작간편,검측령민,결과준학,대우정시호음합제적질량공제구유비상중요적작용화의의.
Objective To establish the simultaneous determination of hesperidin and paeoniflorin in Radix Bupleuri Decoction by RP-HPLC. Method By using the method of ultrasonic extraction, and the phenomenex-ODS chromatographic column ( 250 mm × 4. 60 mm, 5 μm ) , the column temperature was 30-35 ℃, the mobile phase was -1 mL/L phosphoric acid solution ( 15: 85 ) , the detection wavelength of hesperidin and paeoniflorin were 283 nm and 230 nm, and the flow rate was 0. 8-1. 0 mL/min. Results In 0. 021 99-4. 420 μg ( r=0. 999 9 ) range, the linear relationship of hesperidin was good, the average recovery was 95. 00% , RSD was 4. 64% ; in 0. 201 0-1. 002 3 μg ( r=0. 999 9 ) range, the linear relationship of paeoniflorin was good, the average recovery was 98. 04%, RSD was 3. 60%. Conclusion This method has the advantages of simple operation, sensitive detection, accurate results, and has an important role and significance of the quality control of Radix Bupleuri Decoction.
