首都食品与医药
首都食品與醫藥
수도식품여의약
Capital Medicine
2015年
22期
87-89
,共3页
高效液相色谱法%生血宝合剂%芍药苷
高效液相色譜法%生血寶閤劑%芍藥苷
고효액상색보법%생혈보합제%작약감
HPLC%Shengxuebao Granule%Paeoniflorin
目的 建立HPLC法测定生血宝合剂中芍药苷含量的方法.方法 采用高效液相色谱法.色谱柱为Zorbax SBC18,流动相为乙腈-0.1%磷酸(14:86,V/V),检测波长为230nm,流速为1.0ml/min,柱温为25℃,进样量为20μl.结果 芍药苷检测质量浓度在104.2~521.0μg/ml范围内与峰面面积积分值呈良好线性关系(r=0.9972);精密度、稳定性、重复性试验的RSD分别为1.05%、0.47%、1.39%;平均加样回收率为101.3%,RSD=1.80%(n=9).结论 本方法简便可靠、专属性强、重复性好,可用于生血宝合剂的质量控制的标准之一.
目的 建立HPLC法測定生血寶閤劑中芍藥苷含量的方法.方法 採用高效液相色譜法.色譜柱為Zorbax SBC18,流動相為乙腈-0.1%燐痠(14:86,V/V),檢測波長為230nm,流速為1.0ml/min,柱溫為25℃,進樣量為20μl.結果 芍藥苷檢測質量濃度在104.2~521.0μg/ml範圍內與峰麵麵積積分值呈良好線性關繫(r=0.9972);精密度、穩定性、重複性試驗的RSD分彆為1.05%、0.47%、1.39%;平均加樣迴收率為101.3%,RSD=1.80%(n=9).結論 本方法簡便可靠、專屬性彊、重複性好,可用于生血寶閤劑的質量控製的標準之一.
목적 건립HPLC법측정생혈보합제중작약감함량적방법.방법 채용고효액상색보법.색보주위Zorbax SBC18,류동상위을정-0.1%린산(14:86,V/V),검측파장위230nm,류속위1.0ml/min,주온위25℃,진양량위20μl.결과 작약감검측질량농도재104.2~521.0μg/ml범위내여봉면면적적분치정량호선성관계(r=0.9972);정밀도、은정성、중복성시험적RSD분별위1.05%、0.47%、1.39%;평균가양회수솔위101.3%,RSD=1.80%(n=9).결론 본방법간편가고、전속성강、중복성호,가용우생혈보합제적질량공제적표준지일.
Objective To eastablish an HPLC method for determination of Paeoniflorin content in Shengxuebao Granule. Methods: The separation was performed on Zorbax SBC18 column(250 mm×4.6mm,5μm)with acetonitrile-0.1% phosphate (14:86) as mobile phase.The flow rate was 1.0 ml/min, the detection wavelength was 230nm, the column temperature was 25℃, and the injection volume was 20 μl. Results: The liner arrange for Paeoniflorin was104.2~521.0μg/ml, r=0.9972, n=6. RSD of precision,stability and reproducibility tests was respectively 1.05%、0.47%、1.39%. The average recovery of Paeoniflorin was 101.3% and the relative standard deviation was 1.80%(n=9).Conclusions: The method is simple, accurate, sensitive with good reproducibility for analyzing the Paeoniflorin in Shengxuebao Granule and control its quality.
