CAJ | 학술논문

为进一步明确病毒与寄主间的相互作用关系,通过同源比对在烟草细胞中克隆到具有m型硫氧还蛋白(m-type thioredoxin)特征的基因,暂命名为NtTrm.利用原生质体瞬时转化技术、病毒诱导的基因沉默技术(VIGS)、半定量-PCR 以及Northern blot检测技术研究NtTrm的表达以及NtTrm表达与PVY积累的关系.结果表明,在PVY接种10 d后红花大金元叶片中NtTrm表达明显上调;在本氏烟草叶片中NtTrm下调显著促进了PVY在烟草叶片中的积累;而在烟草原生质体中NtTrm过量表达则会明显抑制病毒RNA的积累.初步说明烟草细胞内编码m型硫氧还蛋白的基因受PVY侵染而被诱导表达,这类基因的上调表达可能影响PVY在烟草细胞中的侵染.
위진일보명학병독여기주간적상호작용관계,통과동원비대재연초세포중극륭도구유m형류양환단백(m-type thioredoxin)특정적기인,잠명명위NtTrm.이용원생질체순시전화기술、병독유도적기인침묵기술(VIGS)、반정량-PCR 이급Northern blot검측기술연구NtTrm적표체이급NtTrm표체여PVY적루적관계.결과표명,재PVY접충10 d후홍화대금원협편중NtTrm표체명현상조;재본씨연초협편중NtTrm하조현저촉진료PVY재연초협편중적적루;이재연초원생질체중NtTrm과량표체칙회명현억제병독RNA적적루.초보설명연초세포내편마m형류양환단백적기인수PVY침염이피유도표체,저류기인적상조표체가능영향PVY재연초세포중적침염.
In order to further confirm the interactive relationships between virus and its host, a gene featured the characters of m-type thioredoxin in tobacco cell was cloned by homologous alignment and named as NtTrm. The expression of NtTrm and its relationship with PVY accumulation were studied by protoplast transient transformation, virus-induced gene silencing (VIGS), semi-quantitative RT-PCR and Northern blot test technologies. The results showed that the expression of NtTrm in leaves of cv. Honghuadajinyuan was obviously up-regulated 10 days after PVY inoculation; the down-regulation of NtTrm significantly promoted the accumulation of PVY in leaves of Nicotiana benthamiana; while the over-expression of NtTrm in tobacco protoplast obviously inhibited the accumulation of PVY viral RNA. It preliminarily indicated that NTtrm could be induced by PVY infection and its up-regulated expression might affect the PVY infection in tobacco cells.