CAJ | 학술논문

目的从人肝癌组织中分离CD133+细胞亚群并探讨其致瘤能力.方法将人肝癌组织种植于裸鼠皮下形成移植瘤,将移植瘤标本消化制成单细胞悬液.流式细胞仪检测CD133+的表达.免疫磁珠分选法进一步分离纯化CD133+细胞.免疫荧光检测CD133+细胞亚群的组织表型.对不同亚群细胞进行体外克隆形成实验和裸鼠体内移植瘤形成实验.结果流式细胞仪检测显示,肝癌组织中(4.1±0.6)％的细胞为CD133+细胞.免疫荧光显示分离纯化的细胞中,CD133+细胞占(86.8±7.5)％.体外培养显示CD133+亚群比CD133-亚群具有更强的克隆球形成能力,在裸鼠体内具有更强的肿瘤形成能力(P＜0.05).结论从人肝癌组织中分离的CD133+细胞亚群具有高致瘤性,可能为肝癌干细胞.
목적 종인간암조직중분리CD133+세포아군병탐토기치류능력.방법 장인간암조직충식우라서피하형성이식류,장이식류표본소화제성단세포현액.류식세포의검측CD133+적표체.면역자주분선법진일보분리순화CD133+세포.면역형광검측CD133+세포아군적조직표형.대불동아군세포진행체외극륭형성실험화라서체내이식류형성실험.결과 류식세포의검측현시,간암조직중(4.1±0.6)％적세포위CD133+세포.면역형광현시분리순화적세포중,CD133+세포점(86.8±7.5)％.체외배양현시CD133+아군비CD133-아군구유경강적극륭구형성능력,재라서체내구유경강적종류형성능력(P＜0.05).결론 종인간암조직중분리적CD133+세포아군구유고치류성,가능위간암간세포.
Objective To separate the CD133 + subpopulation in human hepatocellular carcinoma (HCC) and investigate the tumorigenicity.Methods The human liver cancer tissues were subcutaneously transplanted into nude mice to generate xenograft tumors which were then isolated to prepare single cell suspension.The expression of CD133 + subpopulation was further detected using flow cytometry.The CD133 + subpopulations were separated and depurated with magnetic-activated cell sorting system.Immunofluorescence was performed to identify the histological phenotype of CD133 + subpopulation.The in vitro and in vivo clone formation assay and in vivo xenograft formation assay were performed, respectively.Results Flow cytometry analysis revealed that a percentage of (4.1 ± 0.6) ％ CD133 + cells were detected in xenografts.Immunofluorescence studies showed that (86.8 ± 7.5) ％ of the isolated cells were CD133 +.Compared with CD133-population, CD133 + cells showed a higher capability to generate clone sphere in vitro and a higher tumorigenicity in nude mice (P ＜ 0.05).Conclusion The CD133 + subpopulation in human hepatocellular carcinoma had a potent tumorigenicity and was enriched in cancer stem cells.