中华劳动卫生职业病杂志
中華勞動衛生職業病雜誌
중화노동위생직업병잡지
Chinese Journal of Industrial Hygiene and Occupational Diseases
2015年
11期
838-840
,共3页
张志坚%董瑶瑶%黄云帆%彭礼波
張誌堅%董瑤瑤%黃雲帆%彭禮波
장지견%동요요%황운범%팽례파
百草枯%肺纤维化%沙苑子总黄酮%羟脯氨酸
百草枯%肺纖維化%沙苑子總黃酮%羥脯氨痠
백초고%폐섬유화%사원자총황동%간포안산
Paraquat%Pulmonary fibrosis%Total flavonoids from astragalus complanatus%Hydroxyproline
目的 观察沙苑子总黄酮(total flavonoids from astragalus complanatus,FAC)对百草枯(paraquat,PQ)所致大鼠肺纤维化的干预作用并探讨其机制.方法 SD大鼠随机分为正常对照组、PQ染毒组、阳性对照泼尼松组和FAC 40、80、160 mg/kg剂量组.大鼠胃注PQ制备肺纤维化模型后,第2天给予相应药物,每天1次.连续给药28 d后称大鼠体重,处死大鼠检测血清中羟脯氨酸(hydroxyproline,HYP)、血清总抗氧化能力(total antioxidative capacity,T-AOC);取固定部位肺组织切片行HE染色进行病理学观察;蛋白免疫印迹法(Western blot)检测肺组织中仅-平滑肌肌动蛋白(α-SMA)、转化生长因子-β(TGF-β)、Smad2蛋白的表达.结果 沙苑子总黄酮组低、中、高剂量组肺组织Smad2蛋白表达分别为0.31±0.11,0.45±0.12,0.30±0.05,均低于PQ染毒组(0.85±0.34),差异有统计学意义(P＜0.05);沙苑子总黄酮组低、中、高剂量组肺组织α-SMA蛋白表达分别为0.31±0.11、0.35±0.07、0.32±0.10,均低于PQ染毒组(0.45±0.08),差异有统计学意义(P＜0.05);沙苑子总黄酮组低、中、高剂量组肺组织TGF-β蛋白表达分别为0.35±0.04、0.27±0.05、0.18±0.04,均低于PQ染毒组(0.63±0.11),差异有统计学意义(P＜0.05).结论 FAC可能通过抑制TGF-β/Smad信号通路来减轻百草枯致大鼠肺纤维化.
目的 觀察沙苑子總黃酮(total flavonoids from astragalus complanatus,FAC)對百草枯(paraquat,PQ)所緻大鼠肺纖維化的榦預作用併探討其機製.方法 SD大鼠隨機分為正常對照組、PQ染毒組、暘性對照潑尼鬆組和FAC 40、80、160 mg/kg劑量組.大鼠胃註PQ製備肺纖維化模型後,第2天給予相應藥物,每天1次.連續給藥28 d後稱大鼠體重,處死大鼠檢測血清中羥脯氨痠(hydroxyproline,HYP)、血清總抗氧化能力(total antioxidative capacity,T-AOC);取固定部位肺組織切片行HE染色進行病理學觀察;蛋白免疫印跡法(Western blot)檢測肺組織中僅-平滑肌肌動蛋白(α-SMA)、轉化生長因子-β(TGF-β)、Smad2蛋白的錶達.結果 沙苑子總黃酮組低、中、高劑量組肺組織Smad2蛋白錶達分彆為0.31±0.11,0.45±0.12,0.30±0.05,均低于PQ染毒組(0.85±0.34),差異有統計學意義(P＜0.05);沙苑子總黃酮組低、中、高劑量組肺組織α-SMA蛋白錶達分彆為0.31±0.11、0.35±0.07、0.32±0.10,均低于PQ染毒組(0.45±0.08),差異有統計學意義(P＜0.05);沙苑子總黃酮組低、中、高劑量組肺組織TGF-β蛋白錶達分彆為0.35±0.04、0.27±0.05、0.18±0.04,均低于PQ染毒組(0.63±0.11),差異有統計學意義(P＜0.05).結論 FAC可能通過抑製TGF-β/Smad信號通路來減輕百草枯緻大鼠肺纖維化.
목적 관찰사원자총황동(total flavonoids from astragalus complanatus,FAC)대백초고(paraquat,PQ)소치대서폐섬유화적간예작용병탐토기궤제.방법 SD대서수궤분위정상대조조、PQ염독조、양성대조발니송조화FAC 40、80、160 mg/kg제량조.대서위주PQ제비폐섬유화모형후,제2천급여상응약물,매천1차.련속급약28 d후칭대서체중,처사대서검측혈청중간포안산(hydroxyproline,HYP)、혈청총항양화능력(total antioxidative capacity,T-AOC);취고정부위폐조직절편행HE염색진행병이학관찰;단백면역인적법(Western blot)검측폐조직중부-평활기기동단백(α-SMA)、전화생장인자-β(TGF-β)、Smad2단백적표체.결과 사원자총황동조저、중、고제량조폐조직Smad2단백표체분별위0.31±0.11,0.45±0.12,0.30±0.05,균저우PQ염독조(0.85±0.34),차이유통계학의의(P＜0.05);사원자총황동조저、중、고제량조폐조직α-SMA단백표체분별위0.31±0.11、0.35±0.07、0.32±0.10,균저우PQ염독조(0.45±0.08),차이유통계학의의(P＜0.05);사원자총황동조저、중、고제량조폐조직TGF-β단백표체분별위0.35±0.04、0.27±0.05、0.18±0.04,균저우PQ염독조(0.63±0.11),차이유통계학의의(P＜0.05).결론 FAC가능통과억제TGF-β/Smad신호통로래감경백초고치대서폐섬유화.
Objective To investigate the effects of total flavonoids from astragalus complanatus (FAC) on paraquat poisoning-induced pulmonary fibrosis in rats.Methods The rats were divided into six groups randomly:control group,paraquat group,prednisolone group and FAC low-dose,middle-dose,high-dose group.Pulmonary fibrosis model was replicated by intratracheal injection of paraquat.In the mext day,the rats were treated by intragastric administration once a day.After 28 days,the rats were sacrificed.The lung index and the levels of HYP and T-AOC were measured,and the pathologic changes of the lung tissue were obtained by HE staining.The levels of TGF-β,Smad2,α-SMA protein were analyzed by Western blot.Results FAC improved the activity of T-AOC in serum and reduced pulmonary index and the content of HYP as well (P＜0.05 or P＜0.01),the alveolitis and fibrosis extent were attenuated.The expression of Smad2 significantly decreased in groups of FAC low-dose,middle-dose and high-dose (0.31±0.11,0.45±0.12 and 0.30±0.05) as compared with that of the PQ group(0.85±0.34) (P＜0.05).The expression of α-SMA significantly decreased in groups of FAC low-dose,middledose and high-dose (0.31±0.11,0.35±0.07 and 0.32±0.10) as compared with that of the PQ group (0.45±0.08) (P＜0.05).The expression of TGF-β significantly decreased in groups of FAC low-dose,middle-dose and high-dose(0.35±.04,0.27±0.05 and 0.18±0.04) as compared with that of the PQ group(0.63±0.11)(P＜O.05).Conclusion FAC can alleviate PQ-induced pulmonary fibrosis in rats through inhibiting TGF-β/Smad signaling pathway.
