中华眼底病杂志
中華眼底病雜誌
중화안저병잡지
Chinese Journal of Ocular Fundus Diseases
2015年
6期
581-585
,共5页
李晶艳%田敏%张思远%韩佩晏%黄棋%吕红彬
李晶豔%田敏%張思遠%韓珮晏%黃棋%呂紅彬
리정염%전민%장사원%한패안%황기%려홍빈
糖尿病视网膜病变/病理生理学%NF-E2相关因子2%血红素氧化酶(脱环)%动物实验
糖尿病視網膜病變/病理生理學%NF-E2相關因子2%血紅素氧化酶(脫環)%動物實驗
당뇨병시망막병변/병리생이학%NF-E2상관인자2%혈홍소양화매(탈배)%동물실험
Diabetic retinopathy/physiopathology%NF-E2-related factor 2%Heme oxygenase (decyclizing)%Animal experimentation
目的 观察叔丁基对苯二酚(tBHQ)对2型糖尿病(DM)大鼠视网膜组织中核因子E2相关因子(Nrf2)、血红素氧合酶1(HO-1)表达的影响.方法 健康雄性Sprague Dawley大鼠60只,随机抽取20只大鼠为正常对照组(NC组),其余40只大鼠为造模组.造模组大鼠腹腔注射30 mg/kg剂量链脲佐菌素诱导2型DM模型.剔除血糖恢复鼠5只,造模组随机分成DM组、tBHQ组,分别为17、18只大鼠.tBHQ组大鼠给予添加1％tBHQ的高脂高糖饲料喂养.tBHQ喂养后4、12周,苏木精-伊红染色观察大鼠视网膜组织结构;免疫组织化学染色法检测大鼠视网膜组织中Nrf2、HO-1蛋白表达;荧光定量聚合酶链反应(PCR)测定大鼠视网膜组织中Nrf2、HO-1mRNA表达.结果 光学显微镜观察,4周时,tBHQ组大鼠视网膜细胞排列较整齐,个别细胞可见轻度水肿;12周时,视网膜组织结构较清晰,部分细胞水肿.免疫组织化学染色结果显示,4、12周时,DM组大鼠视网膜组织中Nrf2(t=3.115、3.781)、HO-1(f=3.485、3.785)蛋白表达较NC组增多,差异均有统计学意义(P＜0.01).tBHQ组大鼠视网膜组织中Nrf2(t=2.473、2.576)、HO-1(t=2.785、2.879)蛋白表达较DM组增多,差异有统计学意义(P＜0.05).DM组组内比较,12周大鼠视网膜中Nrf2蛋白表达较4周时增高,差异有统计学意义(t=0.276,P＜0.05);tBHQ组组内比较,12周大鼠视网膜中Nrf2(t=2.516)、HO-1(t=2.776)蛋白表达较4周时增高,差异有统计学意义(P＜0.05).荧光定量PCR检测结果显示,4、12同时,DM组大鼠视网膜组织中Nrf2(t=4.758、4.285)、HO-1 mRNA(t=5.114、4.514)表达较NC组增多,差异有统计学意义(P＜0.05);tBHQ组大鼠视网膜组织中Nrf2(t=5.133、4.976)、HO 1 mRNA(t=4.758、4.251)表达较DM组增多,差异有统计学意义(P＜0.05).DM组组内比较,12周时视网膜组织中Nrf2 mRNA表达高于4周,差异有统计学意义(t=5.114,P＜0.05);tBHQ组组内比较,12周时视网膜组织中Nrf2、HO-1 mRNA表达高于4周,差异有统计学意义(t=4.292、4.974,P＜0.05).结论 tBHQ干预可以有效诱导2型DM大鼠视网膜组织中Nrf2、HO-1的表达.
目的 觀察叔丁基對苯二酚(tBHQ)對2型糖尿病(DM)大鼠視網膜組織中覈因子E2相關因子(Nrf2)、血紅素氧閤酶1(HO-1)錶達的影響.方法 健康雄性Sprague Dawley大鼠60隻,隨機抽取20隻大鼠為正常對照組(NC組),其餘40隻大鼠為造模組.造模組大鼠腹腔註射30 mg/kg劑量鏈脲佐菌素誘導2型DM模型.剔除血糖恢複鼠5隻,造模組隨機分成DM組、tBHQ組,分彆為17、18隻大鼠.tBHQ組大鼠給予添加1％tBHQ的高脂高糖飼料餵養.tBHQ餵養後4、12週,囌木精-伊紅染色觀察大鼠視網膜組織結構;免疫組織化學染色法檢測大鼠視網膜組織中Nrf2、HO-1蛋白錶達;熒光定量聚閤酶鏈反應(PCR)測定大鼠視網膜組織中Nrf2、HO-1mRNA錶達.結果 光學顯微鏡觀察,4週時,tBHQ組大鼠視網膜細胞排列較整齊,箇彆細胞可見輕度水腫;12週時,視網膜組織結構較清晰,部分細胞水腫.免疫組織化學染色結果顯示,4、12週時,DM組大鼠視網膜組織中Nrf2(t=3.115、3.781)、HO-1(f=3.485、3.785)蛋白錶達較NC組增多,差異均有統計學意義(P＜0.01).tBHQ組大鼠視網膜組織中Nrf2(t=2.473、2.576)、HO-1(t=2.785、2.879)蛋白錶達較DM組增多,差異有統計學意義(P＜0.05).DM組組內比較,12週大鼠視網膜中Nrf2蛋白錶達較4週時增高,差異有統計學意義(t=0.276,P＜0.05);tBHQ組組內比較,12週大鼠視網膜中Nrf2(t=2.516)、HO-1(t=2.776)蛋白錶達較4週時增高,差異有統計學意義(P＜0.05).熒光定量PCR檢測結果顯示,4、12同時,DM組大鼠視網膜組織中Nrf2(t=4.758、4.285)、HO-1 mRNA(t=5.114、4.514)錶達較NC組增多,差異有統計學意義(P＜0.05);tBHQ組大鼠視網膜組織中Nrf2(t=5.133、4.976)、HO 1 mRNA(t=4.758、4.251)錶達較DM組增多,差異有統計學意義(P＜0.05).DM組組內比較,12週時視網膜組織中Nrf2 mRNA錶達高于4週,差異有統計學意義(t=5.114,P＜0.05);tBHQ組組內比較,12週時視網膜組織中Nrf2、HO-1 mRNA錶達高于4週,差異有統計學意義(t=4.292、4.974,P＜0.05).結論 tBHQ榦預可以有效誘導2型DM大鼠視網膜組織中Nrf2、HO-1的錶達.
목적 관찰숙정기대분이분(tBHQ)대2형당뇨병(DM)대서시망막조직중핵인자E2상관인자(Nrf2)、혈홍소양합매1(HO-1)표체적영향.방법 건강웅성Sprague Dawley대서60지,수궤추취20지대서위정상대조조(NC조),기여40지대서위조모조.조모조대서복강주사30 mg/kg제량련뇨좌균소유도2형DM모형.척제혈당회복서5지,조모조수궤분성DM조、tBHQ조,분별위17、18지대서.tBHQ조대서급여첨가1％tBHQ적고지고당사료위양.tBHQ위양후4、12주,소목정-이홍염색관찰대서시망막조직결구;면역조직화학염색법검측대서시망막조직중Nrf2、HO-1단백표체;형광정량취합매련반응(PCR)측정대서시망막조직중Nrf2、HO-1mRNA표체.결과 광학현미경관찰,4주시,tBHQ조대서시망막세포배렬교정제,개별세포가견경도수종;12주시,시망막조직결구교청석,부분세포수종.면역조직화학염색결과현시,4、12주시,DM조대서시망막조직중Nrf2(t=3.115、3.781)、HO-1(f=3.485、3.785)단백표체교NC조증다,차이균유통계학의의(P＜0.01).tBHQ조대서시망막조직중Nrf2(t=2.473、2.576)、HO-1(t=2.785、2.879)단백표체교DM조증다,차이유통계학의의(P＜0.05).DM조조내비교,12주대서시망막중Nrf2단백표체교4주시증고,차이유통계학의의(t=0.276,P＜0.05);tBHQ조조내비교,12주대서시망막중Nrf2(t=2.516)、HO-1(t=2.776)단백표체교4주시증고,차이유통계학의의(P＜0.05).형광정량PCR검측결과현시,4、12동시,DM조대서시망막조직중Nrf2(t=4.758、4.285)、HO-1 mRNA(t=5.114、4.514)표체교NC조증다,차이유통계학의의(P＜0.05);tBHQ조대서시망막조직중Nrf2(t=5.133、4.976)、HO 1 mRNA(t=4.758、4.251)표체교DM조증다,차이유통계학의의(P＜0.05).DM조조내비교,12주시시망막조직중Nrf2 mRNA표체고우4주,차이유통계학의의(t=5.114,P＜0.05);tBHQ조조내비교,12주시시망막조직중Nrf2、HO-1 mRNA표체고우4주,차이유통계학의의(t=4.292、4.974,P＜0.05).결론 tBHQ간예가이유효유도2형DM대서시망막조직중Nrf2、HO-1적표체.
Objective To observe the effect of tert butyl hydroquinone (tBHQ) on type 2 diabetic rats retinal nuclear factor E2 related factor 2 (Nrf2) and hemeoxygenase-1 (HO-1).Methods 60 Sprague Dawley rats were randomly divided into normal control group (NC group, n=20) and model group (n=40).The rats in model group were intraperitoneal injected with streptozotocin (30 mg/kg) to establishing type 2 diabetic mellitus (DM).There were 35 rats successfully established and they were randomly divided into diabetic group (DM group, 17 rats) and tBHQ group (18 rats).The rats in tBHQ group were fed with high fat and sugar diet with 1％ tBHQ.After 4 weeks and 12 weeks of tBHQ intervention, hematoxylin eosin staining of retinal sections, immunohistochemical staining and quantitative polymerase chain reaction (PCR) of Nrf2 and HO-1 were performed.Results In tBHQ control, the retina of rats was normal and individual cells showed slightly edema at 4 weeks;the retinal structure of rats was clear and part of cells showed edema at 12 weeks.At 4 and 12 weeks, the expression of Nrf2 (t=3.115, 3.781) and HO-1 (t=3.485, 3.785) protein in DM group were higher than that in NC group (P＜0.05);the expression of Nrf2 (t=2.473, 2.576) and HO-1 (t=2.785, 2.879) protein in tBHQ group were higher than that in DM group (P＜0.05).In DM group, the expression of Nrf2 protein at 12 weeks was higher than that at 4 weeks (t=0.276, P＜0.05).In tBHQ group, the expression of Nrf2 (t=2.516) and HO-1 (t=2.776) protein at 12 weeks were higher than that at 4 weeks (P＜0.05).4 and 12 weeks, the expression of Nrf2 (t=4.758,4.285) and HO-1 (t=5.114, 4.514) mRNA in DM group were higher than that in NC group (P＜0.05);the expression of Nrf2 (t=5.133, 4.976) and HO-1 (t=4.758, 4.251) mRNA in tBHQ group were higher than that in DM group (P＜0.05).In DM gruop, the expression of Nrf2 protein at 12 weeks was higher than that at 4 weeks (t=5.114, P＜0.05).In tBHQ group, the expression of Nrf2 (t=4.292) and HO-1 (t=4.974) protein at 12 weeks were higher than that at 4 weeks (P ＜ 0.05).Conclusion tBHQ intervention can increased the expression of Nrf2, HO-1 significantly in the retina of type 2 diabetic rats.
