中华劳动卫生职业病杂志
中華勞動衛生職業病雜誌
중화노동위생직업병잡지
Chinese Journal of Industrial Hygiene and Occupational Diseases
2015年
11期
841-843
,共3页
王东强%张平平%李志军%刘颖
王東彊%張平平%李誌軍%劉穎
왕동강%장평평%리지군%류영
甲钴胺%周围神经%神经元%基因,bcl-2
甲鈷胺%週圍神經%神經元%基因,bcl-2
갑고알%주위신경%신경원%기인,bcl-2
Mecobalamin%Peripheral nerve%Neurons%Genes,bcl-2
目的 观察甲钴胺对周围神经损伤后神经元细胞Bax和Bcl-2凋亡蛋白表达的影响,探讨神经细胞凋亡在周围神经损伤后再生中的作用.方法 以30只健康成年雄性Wistar大鼠为研究对象,随机分为假手术组、模型组和甲钴胺组,每组10只,建立左侧坐骨神经钳夹半损伤模型,甲钴胺组给予甲钴胺灌胃,对照组、模型组给予等量生理盐水灌胃,术后14d观察神经元细胞Bax蛋白、Bcl-2蛋白的表达,用图像分析技术对神经元细胞Bax及Bcl-2蛋白进行半定量分析.结果 与假手术组比较,模型组脊髓前角运动神经元和神经节感觉神经元Bax蛋白表达增强,模型组脊髓前角运动神经元Bcl-2蛋白表达减弱,神经节感觉神经元Bcl-2蛋白表达增强,差异有统计学意义(P＜0.05);甲钴胺组脊髓前角运动神经元和神经节感觉神经元Bax表达蛋白减弱,Bcl-2蛋白表达增强,与模型组比较,差异均有统计学意义(P＜0.05).结论 甲钴胺具有抗凋亡作用,可能通过抑制受损神经元的死亡,为神经功能的恢复创造了条件.
目的 觀察甲鈷胺對週圍神經損傷後神經元細胞Bax和Bcl-2凋亡蛋白錶達的影響,探討神經細胞凋亡在週圍神經損傷後再生中的作用.方法 以30隻健康成年雄性Wistar大鼠為研究對象,隨機分為假手術組、模型組和甲鈷胺組,每組10隻,建立左側坐骨神經鉗夾半損傷模型,甲鈷胺組給予甲鈷胺灌胃,對照組、模型組給予等量生理鹽水灌胃,術後14d觀察神經元細胞Bax蛋白、Bcl-2蛋白的錶達,用圖像分析技術對神經元細胞Bax及Bcl-2蛋白進行半定量分析.結果 與假手術組比較,模型組脊髓前角運動神經元和神經節感覺神經元Bax蛋白錶達增彊,模型組脊髓前角運動神經元Bcl-2蛋白錶達減弱,神經節感覺神經元Bcl-2蛋白錶達增彊,差異有統計學意義(P＜0.05);甲鈷胺組脊髓前角運動神經元和神經節感覺神經元Bax錶達蛋白減弱,Bcl-2蛋白錶達增彊,與模型組比較,差異均有統計學意義(P＜0.05).結論 甲鈷胺具有抗凋亡作用,可能通過抑製受損神經元的死亡,為神經功能的恢複創造瞭條件.
목적 관찰갑고알대주위신경손상후신경원세포Bax화Bcl-2조망단백표체적영향,탐토신경세포조망재주위신경손상후재생중적작용.방법 이30지건강성년웅성Wistar대서위연구대상,수궤분위가수술조、모형조화갑고알조,매조10지,건립좌측좌골신경겸협반손상모형,갑고알조급여갑고알관위,대조조、모형조급여등량생리염수관위,술후14d관찰신경원세포Bax단백、Bcl-2단백적표체,용도상분석기술대신경원세포Bax급Bcl-2단백진행반정량분석.결과 여가수술조비교,모형조척수전각운동신경원화신경절감각신경원Bax단백표체증강,모형조척수전각운동신경원Bcl-2단백표체감약,신경절감각신경원Bcl-2단백표체증강,차이유통계학의의(P＜0.05);갑고알조척수전각운동신경원화신경절감각신경원Bax표체단백감약,Bcl-2단백표체증강,여모형조비교,차이균유통계학의의(P＜0.05).결론 갑고알구유항조망작용,가능통과억제수손신경원적사망,위신경공능적회복창조료조건.
Objective To observe the effects of mecobalamin on the expression of apoptosis-related proteins,Bax and Bcl-2,in neurons after peripheral nerve injury,and to explore the role of neuron apoptosis in peripheral nerve regeneration after injury.Methods Thirty healthy adult male wistar rats were randomly divided into sham-operation group,model group,and mecobalamin group,with 10 rats in each group.A rat model of left sciatic nerve semi-injury was established using forceps.Rats in the mecobalamin group were fed mecobalamin,while rats in the sham-operation group and model group were given the same dose of normal saline.The protein expression of Bax and Bcl-2 in neurons was measured at 14 days after operation.A semi-quantitative analysis of Bax and Bcl-2 proteins was performed by image analysis technology.Results The model group had significantly increased Bax protein expression and significantly reduced Bcl-2 protein expression in spinal cord anterior horn motor neurons and ganglion sensory neurons compared with the sham-operation group (P＜0.05).Compared with the model group and sham-operation group,the mecobalamin group had significantly reduced Bax protein expression and significantly increased Bcl-2 protein expression in spinal cord anterior horn motor neurons and ganglion sensory neurons (P＜0.05).Conclusion Mecobalamin has anti-apoptotic effect,and it contributes to neurological function recovery possibly by inhibiting the death of injured neurons.
