中华眼底病杂志
中華眼底病雜誌
중화안저병잡지
Chinese Journal of Ocular Fundus Diseases
2015年
6期
571-574
,共4页
谢国丽%傅强%赵露%王艳玲
謝國麗%傅彊%趙露%王豔玲
사국려%부강%조로%왕염령
缺血/病理生理学%细胞凋亡%半胱氨酸天冬氨酸蛋白酶%bcl-2相关Ⅹ蛋白质%动物实验
缺血/病理生理學%細胞凋亡%半胱氨痠天鼕氨痠蛋白酶%bcl-2相關Ⅹ蛋白質%動物實驗
결혈/병리생이학%세포조망%반광안산천동안산단백매%bcl-2상관Ⅹ단백질%동물실험
Ischemia/physiopathology%Apoptosis%Caspase%bcl-2-associated Ⅹ protein%Animal experimentation
目的 观察眼缺血综合征(OIS)大鼠视网膜组织中促凋亡相关蛋白半胱氨酸蛋白酶(Caspase)3、Caspase 8、B细胞淋巴瘤/白血病-2(Bcl-2)相关Ⅹ蛋白(Bax),抑制凋亡相关蛋白Bcl-2的表达.方法Brown Norway大鼠30只随机分为实验组、对照组,每组均为15只大鼠.实验组大鼠行双侧颈总动脉结扎建立OIS动物模型;对照组大鼠游离双侧颈总动脉,但不结扎.建模后3个月,行苏木精-伊红染色,观察大鼠视网膜各层厚度及神经节细胞(RGC)数量;蛋白质免疫印迹法、实时定量聚合酶链反应检测,观察大鼠视网膜中Caspase-3、Caspase 8、Bax以及Bcb2蛋白及基因表达.结果 与对照组相比,实验组大鼠RGC数量明显减少,差异有统计学意义(t=3.059,P＜0.01);视网膜总厚度(t=3.036)及内丛状层(t=3.715)、内核层(t=3.339)、外丛状层(t=3.341)厚度均明显降低,差异有统计学意义(P＜o.05);外核层厚度无明显变化,差异无统计学意义(t=2.000,P＞0.05).实验组大鼠视网膜Caspase-3、Caspase-8、Bax蛋白(F=17.036、7.787、11.431)及基因(F=11.162、17.763、12.183)表达均显著升高,差异有统计学意义(P＜0.05);Bcl-2蛋白(F=10.298)及基因(F=12.047)表达显著下降,差异有统计学意义(P＜0.05).结论凋亡相关蛋白在OIS大鼠视网膜组织中显著表达;促凋亡相关蛋白表达升高,抑制凋亡蛋白表达下降.
目的 觀察眼缺血綜閤徵(OIS)大鼠視網膜組織中促凋亡相關蛋白半胱氨痠蛋白酶(Caspase)3、Caspase 8、B細胞淋巴瘤/白血病-2(Bcl-2)相關Ⅹ蛋白(Bax),抑製凋亡相關蛋白Bcl-2的錶達.方法Brown Norway大鼠30隻隨機分為實驗組、對照組,每組均為15隻大鼠.實驗組大鼠行雙側頸總動脈結扎建立OIS動物模型;對照組大鼠遊離雙側頸總動脈,但不結扎.建模後3箇月,行囌木精-伊紅染色,觀察大鼠視網膜各層厚度及神經節細胞(RGC)數量;蛋白質免疫印跡法、實時定量聚閤酶鏈反應檢測,觀察大鼠視網膜中Caspase-3、Caspase 8、Bax以及Bcb2蛋白及基因錶達.結果 與對照組相比,實驗組大鼠RGC數量明顯減少,差異有統計學意義(t=3.059,P＜0.01);視網膜總厚度(t=3.036)及內叢狀層(t=3.715)、內覈層(t=3.339)、外叢狀層(t=3.341)厚度均明顯降低,差異有統計學意義(P＜o.05);外覈層厚度無明顯變化,差異無統計學意義(t=2.000,P＞0.05).實驗組大鼠視網膜Caspase-3、Caspase-8、Bax蛋白(F=17.036、7.787、11.431)及基因(F=11.162、17.763、12.183)錶達均顯著升高,差異有統計學意義(P＜0.05);Bcl-2蛋白(F=10.298)及基因(F=12.047)錶達顯著下降,差異有統計學意義(P＜0.05).結論凋亡相關蛋白在OIS大鼠視網膜組織中顯著錶達;促凋亡相關蛋白錶達升高,抑製凋亡蛋白錶達下降.
목적 관찰안결혈종합정(OIS)대서시망막조직중촉조망상관단백반광안산단백매(Caspase)3、Caspase 8、B세포림파류/백혈병-2(Bcl-2)상관Ⅹ단백(Bax),억제조망상관단백Bcl-2적표체.방법Brown Norway대서30지수궤분위실험조、대조조,매조균위15지대서.실험조대서행쌍측경총동맥결찰건립OIS동물모형;대조조대서유리쌍측경총동맥,단불결찰.건모후3개월,행소목정-이홍염색,관찰대서시망막각층후도급신경절세포(RGC)수량;단백질면역인적법、실시정량취합매련반응검측,관찰대서시망막중Caspase-3、Caspase 8、Bax이급Bcb2단백급기인표체.결과 여대조조상비,실험조대서RGC수량명현감소,차이유통계학의의(t=3.059,P＜0.01);시망막총후도(t=3.036)급내총상층(t=3.715)、내핵층(t=3.339)、외총상층(t=3.341)후도균명현강저,차이유통계학의의(P＜o.05);외핵층후도무명현변화,차이무통계학의의(t=2.000,P＞0.05).실험조대서시망막Caspase-3、Caspase-8、Bax단백(F=17.036、7.787、11.431)급기인(F=11.162、17.763、12.183)표체균현저승고,차이유통계학의의(P＜0.05);Bcl-2단백(F=10.298)급기인(F=12.047)표체현저하강,차이유통계학의의(P＜0.05).결론조망상관단백재OIS대서시망막조직중현저표체;촉조망상관단백표체승고,억제조망단백표체하강.
Objective To observe the expression of Caspase-3, Caspase-8, Bcl-2, Bax in the rat retina of ocular ischemic syndrome (OIS).Methods 30 Brown Norway rats were randomly divided into experimental group and control group, 15 rats in each group.The rats in experimental group were established a model through ligating the bilateral common carotid artery.At 3 months after modeling, the retinal thickness and ganglion cell (RGC) density were measured by hematoxylin eosin staining;the expression of Caspase 3, Caspase 8, Bax and Bcl-2 in the retina was measured by quantitative real-time reverse transcription polymerase chain rcaction.Results The RGC density in control group and experimental group was 61.97±9.07 and 38.1 ±5.98, respectively.Compared to the control group, the RGC density was diminished in the experimental group (t=3.059, P＜0.01).A significant decrease was found in the total retina (t=3.036), inner plexiform (t=3.715), inner nuclear (t=3.339) and outer plexiform (t=3.341) thickness (P＜0.05).However, no change of the thickness was evident in the outer nuclear layers (t=2.000, P＞0.05).The levels of protein and RNA expression of Caspase 3, Caspase 8 and Bax in the retina were increased in experimental group (F=17.036, 7.787, 11.431, 11.162, 17.763, 12.183;P＜0.05), while the Bcl-2 expression were decreased (F=10.298, 12.047;P＜0.05).Conclusions There is obvious expression of apoptosis-associated proteins in the rat retina of OIS.Caspase 3, Caspase 8 and Bax expression are increased, while Bcl-2 expression are decreased.
