中国综合临床
中國綜閤臨床
중국종합림상
Clinical Medicine of China
2015年
12期
1071-1074
,共4页
白莲梅%王晓晓%马玉珍%任宇
白蓮梅%王曉曉%馬玉珍%任宇
백련매%왕효효%마옥진%임우
肿瘤坏死因子α%瘦素%非酒精性脂肪性肝病
腫瘤壞死因子α%瘦素%非酒精性脂肪性肝病
종류배사인자α%수소%비주정성지방성간병
Tumor necrosis factor alpha%Leptin%Nonaloholic fatty liver disease
目的 明确肿瘤坏死因子α(TNF-α)和瘦素(Leptin)在非酒精性脂肪性肝病中的相关性.方法 利用实时定量PCR(q-PCR)和酶联免疫吸附实验(ELISA)测定正常组、非酒精性单纯性脂肪肝(NAFL)、非酒精性脂肪性肝炎(NASH)及其相关肝硬化中白细胞及血清中TNF-α和Leptin mRNA和蛋白表达量的变化,并研究两者之间的相关性.结果 在mRNA水平上,肝硬化组中TNF-α转录量最高分别是正常组、NAFL组和NASH组的14.03倍、12.07倍和11.05倍,且差异均有统计学意义(P均＜0.05),Leptin转录量分别是正常组、NAFL组和NASH组的1.95倍0.79倍和1.45倍,差异均无统计学意义(P均＞0.05);且在肝硬化组中,TNF-α转录量是Leptin的7.52倍,差异有统计学意义(P＜0.01).在蛋白表达水平上,肝硬化组中TNF-α和Leptin蛋白表达量分别较正常组高1.98倍和2.39倍,较NAFL组高1.24倍和1.30倍,较NASH组高1.27倍和1.37倍,且差异均有统计学意义(P均＜0.05),而且在肝硬化进展的过程中TNF-α表达量显著高于Leptin呈递增趋势(P＜0.01).结论 TNF-α和Leptin在淋巴细胞中表达中少量转录,但在血清中高表达,而且TNF-α和Leptin影响NAFLD的演变,并呈现出一种正相关关系,两者相互作用导致NAFLD的发生.
目的 明確腫瘤壞死因子α(TNF-α)和瘦素(Leptin)在非酒精性脂肪性肝病中的相關性.方法 利用實時定量PCR(q-PCR)和酶聯免疫吸附實驗(ELISA)測定正常組、非酒精性單純性脂肪肝(NAFL)、非酒精性脂肪性肝炎(NASH)及其相關肝硬化中白細胞及血清中TNF-α和Leptin mRNA和蛋白錶達量的變化,併研究兩者之間的相關性.結果 在mRNA水平上,肝硬化組中TNF-α轉錄量最高分彆是正常組、NAFL組和NASH組的14.03倍、12.07倍和11.05倍,且差異均有統計學意義(P均＜0.05),Leptin轉錄量分彆是正常組、NAFL組和NASH組的1.95倍0.79倍和1.45倍,差異均無統計學意義(P均＞0.05);且在肝硬化組中,TNF-α轉錄量是Leptin的7.52倍,差異有統計學意義(P＜0.01).在蛋白錶達水平上,肝硬化組中TNF-α和Leptin蛋白錶達量分彆較正常組高1.98倍和2.39倍,較NAFL組高1.24倍和1.30倍,較NASH組高1.27倍和1.37倍,且差異均有統計學意義(P均＜0.05),而且在肝硬化進展的過程中TNF-α錶達量顯著高于Leptin呈遞增趨勢(P＜0.01).結論 TNF-α和Leptin在淋巴細胞中錶達中少量轉錄,但在血清中高錶達,而且TNF-α和Leptin影響NAFLD的縯變,併呈現齣一種正相關關繫,兩者相互作用導緻NAFLD的髮生.
목적 명학종류배사인자α(TNF-α)화수소(Leptin)재비주정성지방성간병중적상관성.방법 이용실시정량PCR(q-PCR)화매련면역흡부실험(ELISA)측정정상조、비주정성단순성지방간(NAFL)、비주정성지방성간염(NASH)급기상관간경화중백세포급혈청중TNF-α화Leptin mRNA화단백표체량적변화,병연구량자지간적상관성.결과 재mRNA수평상,간경화조중TNF-α전록량최고분별시정상조、NAFL조화NASH조적14.03배、12.07배화11.05배,차차이균유통계학의의(P균＜0.05),Leptin전록량분별시정상조、NAFL조화NASH조적1.95배0.79배화1.45배,차이균무통계학의의(P균＞0.05);차재간경화조중,TNF-α전록량시Leptin적7.52배,차이유통계학의의(P＜0.01).재단백표체수평상,간경화조중TNF-α화Leptin단백표체량분별교정상조고1.98배화2.39배,교NAFL조고1.24배화1.30배,교NASH조고1.27배화1.37배,차차이균유통계학의의(P균＜0.05),이차재간경화진전적과정중TNF-α표체량현저고우Leptin정체증추세(P＜0.01).결론 TNF-α화Leptin재림파세포중표체중소량전록,단재혈청중고표체,이차TNF-α화Leptin영향NAFLD적연변,병정현출일충정상관관계,량자상호작용도치NAFLD적발생.
Objective To clarify the relationship between tumor necrosis factor alpha (TNF-α) and leptin in nonalcoholic fatty liver disease.Methods The real-time quantitative PCR (q-PCR) and enzymelinked immunosorbent adsorption experiment(ELISA) were used to detect the expression and correlation of TNFα and leptin in blood cells and serum from the normal group, non-alcoholic simple fatty liver(NAFL) group, nonalcoholic steatohepatitis (NASH) group and cirrhosis group.Results At the level of mRNA, the transcription of TNF-α in cirrhosis group was 14.03 times, 12.07 times and 11.05 times of the normal group, NAFL group and NASH group,and the difference was significant(P＜0.05).Leptin transcription of cirrhosis group was 1.95 times,0.79 times and 1.45 times of normal group, NAFL group and of NASH group(P＞0.05).And in the cirrhosis group, the expression of TNF-α was 7.52 times higher than Leptin (P＜ 0.01).In expression level of protein,TNF-α and leptin in cirrhosis group was 1.98 times and 2.39 times higher than the normal group, 1.24 times and 1.30 times higher than the NAFL group, 1.27 times and 1.37 times higher than NASH, and the difference was significant(P＜0.05).Moreover the expression of TNF-α was significantly higher than that of Leptinin groups above(P＜0.01).Conclusion TNF-α and Leptin are less expression in lymphocytes, but more expression in serum.And TNF-α and Leptin affect the evolution of NAFLD, and present a positive correlation, which lead to the occurrence of NAFLD.Comparing the two methods, detection of serum is more sensitive and more suitable for clinical study than lymphocyte.
