CAJ | 학술논문

采用UPLC-PDA法测定不同采收期白花延龄草根茎及茎叶中重楼皂苷Ⅵ及偏诺皂苷元 -3-O-α-L-吡喃鼠李糖基(1→4)-[α-L-吡喃鼠李糖基(1→2)]-β-D -葡萄糖苷的含量.色谱柱 :Waters acquity UPLC BEH C18 (2.1mm × 100mm , 1.7μm );检测器 :PDA;流动相 :乙腈(A )-水(B );流速 :0.4mL/min;线性梯度洗脱 :0min~5min ,40% A~47% A;柱温 :35℃ ;检测波长:203nm;进样量 :2μL.3个时期白花延龄草根茎中重楼皂苷Ⅵ含量分别为1.06% 、1.19% 和1.35% ,茎叶中含量分别为0.019% 、0.010% 和0.002% ;根茎中偏诺皂苷元-3-O-α-L-吡喃鼠李糖基(1→4 )-[α-L-吡喃鼠李糖基(1→2 )]-β-D-葡萄糖苷含量分别为0.91% 、0.97% 和1.12% ,茎叶中含量分别为0.064% 、0.049% 和0.012% .
채용UPLC-PDA법측정불동채수기백화연령초근경급경협중중루조감Ⅵ급편낙조감원 -3-O-α-L-필남서리당기(1→4)-[α-L-필남서리당기(1→2)]-β-D -포도당감적함량.색보주 :Waters acquity UPLC BEH C18 (2.1mm × 100mm , 1.7μm );검측기 :PDA;류동상 :을정(A )-수(B );류속 :0.4mL/min;선성제도세탈 :0min~5min ,40% A~47% A;주온 :35℃ ;검측파장:203nm;진양량 :2μL.3개시기백화연령초근경중중루조감Ⅵ함량분별위1.06% 、1.19% 화1.35% ,경협중함량분별위0.019% 、0.010% 화0.002% ;근경중편낙조감원-3-O-α-L-필남서리당기(1→4 )-[α-L-필남서리당기(1→2 )]-β-D-포도당감함량분별위0.91% 、0.97% 화1.12% ,경협중함량분별위0.064% 、0.049% 화0.012% .
The content of polyphyllin Ⅵ and pennogenin-3-O-α-L-rhamnopyranosyl -(-PDA ,which were seperated from the rhizome and stem leaf of white flowered trillium got in different harvest time.Waters Acquity UPLC BEH C18 column (1.7μm ,2.1mm × 100mm ) was used ,PDA was detector ,the mobile phase was composed of Acetonitrile(A)-Water(B) ,the flow rate was 0.4mL/min ,and the detection wave-length was 203nm.The content of polyphyllin Ⅵ in white flowered trillium rhizomem and stem leave got in three period were 1.06% ,1.19% , 1.35% and 0.019% ,0.010% ,0.002% respectively ,and the content of pennogenin-3-O-α-L-rhamnopyranosyl -(1→4 )-[α-L-rhamnopyranosyl -(1→2)]-β-D-glucopyranoside were 0.91% ,0.97% ,1.12% and 0.064% ,0.049% ,0.012% respectively.