CAJ | 학술논문

以山东马尾松的破壁松花粉水提物为原材料 ,采用苯酚 -浓硫酸法 ,通过紫外分光光度法测定破壁松花粉水提物总糖的含量 ,并从精密度、重复性、稳定性以及加样回收率等方面进行了方法学考察.结果表明 ,破壁松花粉水提物中含有丰富的总糖 ,含量为60.22% ,在0.0025mg/mL~0.0125mg/mL的范围内总糖的浓度与吸光度呈现良好的线性关系( r=0.9991 ,n=5 ) ,平均回收率为98.38% (RSD% =1.72 ,n=6 ).该方法操作简便、快速 ,准确度、重复性和稳定性较好 ,可用于破壁松花粉水提物中总糖含量测定 ,并为中药和天然产物中总糖的含量测定提供参考.
이산동마미송적파벽송화분수제물위원재료 ,채용분분 -농류산법 ,통과자외분광광도법측정파벽송화분수제물총당적함량 ,병종정밀도、중복성、은정성이급가양회수솔등방면진행료방법학고찰.결과표명 ,파벽송화분수제물중함유봉부적총당 ,함량위60.22% ,재0.0025mg/mL~0.0125mg/mL적범위내총당적농도여흡광도정현량호적선성관계( r=0.9991 ,n=5 ) ,평균회수솔위98.38% (RSD% =1.72 ,n=6 ).해방법조작간편、쾌속 ,준학도、중복성화은정성교호 ,가용우파벽송화분수제물중총당함량측정 ,병위중약화천연산물중총당적함량측정제공삼고.
The feasibility of aqueous extract from wall -broken pinus massoniana pollen as main material to extract the total sugar was assessed in terms of accuracy ,repetitive ,stability and sample recovery rate through the ultraviolet spectrophotometry and phenol -sulfuric acid method.. The result showed 60.22% total sugar was extracted from aqueous extract from wall -broken pius massioniana pollen.There had a liner relation between concentration and absorbance when total sugar limited to 0.0025mg/mL-0.0125mg/mL(r=0.9991 ,n=5) ,while the average re-covery rate was 98.38% (RSD% =1.72 ,n=6 ).This method is simple ,accurate ,repeatable ,stable and may be used as a reference method for the determination of total sugar in herbal materials or natural products.