特产研究
特產研究
특산연구
Special Wild Economic Animal and Plant Research
2015年
4期
45-48
,共4页
逄世峰%闫梅霞%崔丽丽%李亚丽%郭靖%YANG Jun
逄世峰%閆梅霞%崔麗麗%李亞麗%郭靖%YANG Jun
방세봉%염매하%최려려%리아려%곽정%YANG Jun
超高效液相色谱%蛹虫草%腺苷%虫草素
超高效液相色譜%蛹蟲草%腺苷%蟲草素
초고효액상색보%용충초%선감%충초소
UPLC%Cordyceps militaris%adenosine%cordycepin
建立蛹虫草子实体中腺苷和虫草素的UPLC检测方法.色谱柱 :ACQUITY UPLC HSS T3 (2.1mm × 50mm ,1.8μm );流动相 :甲醇-水(15:85 ,v/v );流速 :0.3mL/min;柱温 :35℃ ;检测波长 :260nm;进样量:2μL.蛹虫草子实体中腺苷和虫草素分别在1.12ng~22.4ng、0.34ng~6.8ng范围内线性关系良好 ,平均加样回收率分别为101.53% 、95.79% .该方法准确性高、重复性好 ,可用于蛹虫草子实体中腺苷和虫草素含量测定.
建立蛹蟲草子實體中腺苷和蟲草素的UPLC檢測方法.色譜柱 :ACQUITY UPLC HSS T3 (2.1mm × 50mm ,1.8μm );流動相 :甲醇-水(15:85 ,v/v );流速 :0.3mL/min;柱溫 :35℃ ;檢測波長 :260nm;進樣量:2μL.蛹蟲草子實體中腺苷和蟲草素分彆在1.12ng~22.4ng、0.34ng~6.8ng範圍內線性關繫良好 ,平均加樣迴收率分彆為101.53% 、95.79% .該方法準確性高、重複性好 ,可用于蛹蟲草子實體中腺苷和蟲草素含量測定.
건립용충초자실체중선감화충초소적UPLC검측방법.색보주 :ACQUITY UPLC HSS T3 (2.1mm × 50mm ,1.8μm );류동상 :갑순-수(15:85 ,v/v );류속 :0.3mL/min;주온 :35℃ ;검측파장 :260nm;진양량:2μL.용충초자실체중선감화충초소분별재1.12ng~22.4ng、0.34ng~6.8ng범위내선성관계량호 ,평균가양회수솔분별위101.53% 、95.79% .해방법준학성고、중복성호 ,가용우용충초자실체중선감화충초소함량측정.
The quantitative analysis method for adenosine and cordycepin in Cordyceps militaris were established by UPLC.The chromatographic separation was achieved on a ACQUITY UPLC HSS T3 column(2.1mm × 50mm ,1.8μm) ,using methanol -water(15:85 ,v/v)as the mobile phase with a flow rate of 0.3mL/min at 35℃.The column temperature was set at 30℃ ,and the eluate was detected at 260nm.Quantitative analysis of UPLC showed that the linear ranges of adenosine and cordycepin were 1.12ng-22.4ng ,0.34ng-6.8ng respectively.The average recoveries of adenosine and cordycepin were 101.53% and 95.79% respectively.The method set up is accurate ,feasible and reliable ,can be applied for detection of adenosine and cordycepin in C.militaris.
