特产研究
特產研究
특산연구
Special Wild Economic Animal and Plant Research
2015年
4期
23-25
,共3页
柴石解郁片%黄芪甲苷%高效液相色谱-蒸发光散射检测法%含量测定
柴石解鬱片%黃芪甲苷%高效液相色譜-蒸髮光散射檢測法%含量測定
시석해욱편%황기갑감%고효액상색보-증발광산사검측법%함량측정
chaishijieyu tablets%astragnaloside%HPLC-ELSD%content determination
建立以HPLC-ELSD法测定柴石解郁片中黄芪甲苷含量的方法.色谱柱 :Diamonsil C18 ;流动相:乙腈-水(32:68 );流速 :1mL/min;蒸发光散色器漂移管温度 :105℃ ;雾化气体 :氮气 ;流速 :3.0L/min.结果表明 ,黄芪甲苷线性范围1.024μg~5.12μg ,平均回收率97.59% ,RSD=0.92% (n=6 ).本方法灵敏、准确、重现性好 ,可用于柴石解郁片的质量控制.
建立以HPLC-ELSD法測定柴石解鬱片中黃芪甲苷含量的方法.色譜柱 :Diamonsil C18 ;流動相:乙腈-水(32:68 );流速 :1mL/min;蒸髮光散色器漂移管溫度 :105℃ ;霧化氣體 :氮氣 ;流速 :3.0L/min.結果錶明 ,黃芪甲苷線性範圍1.024μg~5.12μg ,平均迴收率97.59% ,RSD=0.92% (n=6 ).本方法靈敏、準確、重現性好 ,可用于柴石解鬱片的質量控製.
건립이HPLC-ELSD법측정시석해욱편중황기갑감함량적방법.색보주 :Diamonsil C18 ;류동상:을정-수(32:68 );류속 :1mL/min;증발광산색기표이관온도 :105℃ ;무화기체 :담기 ;류속 :3.0L/min.결과표명 ,황기갑감선성범위1.024μg~5.12μg ,평균회수솔97.59% ,RSD=0.92% (n=6 ).본방법령민、준학、중현성호 ,가용우시석해욱편적질량공제.
To develop a method for the content determination of astragnaloside in Chai Shi Jie Yu tablets.The chromatography column was oc-tadecylsilane chemically bonded silica as the filler ,the moving phase was acetonitrile -water(32:68) ,the flow velocity was 1mL one minute , the drift tube temperature of evaporative light -scattering detector was 105℃ ,air or nitrogen was as the atomizing gas ,its flow velocity was 3.0L one minute.Results:The incoming sample amount of astragnaloside was 1.024μg-5.12μg ,the average recovery was 97.59% ,RSD=0.92% (n=6 ).This method is sensitive ,accurate ,and has good reproducibility.It can be used for the content determination of astragnaloside in Chai Shi Jie Yu tablet.
