特产研究
特產研究
특산연구
Special Wild Economic Animal and Plant Research
2015年
4期
38-40
,共3页
张秀莲%赵卉%张志东%许世泉%李红%张影
張秀蓮%趙卉%張誌東%許世泉%李紅%張影
장수련%조훼%장지동%허세천%리홍%장영
杜马斯燃烧法%凯氏定氮法%人参%蛋白质含量
杜馬斯燃燒法%凱氏定氮法%人參%蛋白質含量
두마사연소법%개씨정담법%인삼%단백질함량
Combustion method%Kjeldahl method%Panax ginseng C .A .Mey%protein content
选取14个人参样品和2种化学标准物作为试验材料 ,分别采用杜马斯燃烧法和凯氏定氮法测定16个试验样品中的蛋白质含量.结果显示 ,2种测定方法测得的人参中蛋白质含量结果差异不显著( P>0.05 ) ,凯氏定氮法的测定值平均变异系数CV=2.010% ,杜马斯燃烧法测定值平均变异系数CV=1.156% ,2组变异系数均小于5% .大部分样品的杜马斯燃烧法测定值高于凯氏定氮法测定值.2种方法测定值比值的平均值C/K=1.023.综上 ,杜马斯燃烧法可以作为人参蛋白质含量的常规检测方法.
選取14箇人參樣品和2種化學標準物作為試驗材料 ,分彆採用杜馬斯燃燒法和凱氏定氮法測定16箇試驗樣品中的蛋白質含量.結果顯示 ,2種測定方法測得的人參中蛋白質含量結果差異不顯著( P>0.05 ) ,凱氏定氮法的測定值平均變異繫數CV=2.010% ,杜馬斯燃燒法測定值平均變異繫數CV=1.156% ,2組變異繫數均小于5% .大部分樣品的杜馬斯燃燒法測定值高于凱氏定氮法測定值.2種方法測定值比值的平均值C/K=1.023.綜上 ,杜馬斯燃燒法可以作為人參蛋白質含量的常規檢測方法.
선취14개인삼양품화2충화학표준물작위시험재료 ,분별채용두마사연소법화개씨정담법측정16개시험양품중적단백질함량.결과현시 ,2충측정방법측득적인삼중단백질함량결과차이불현저( P>0.05 ) ,개씨정담법적측정치평균변이계수CV=2.010% ,두마사연소법측정치평균변이계수CV=1.156% ,2조변이계수균소우5% .대부분양품적두마사연소법측정치고우개씨정담법측정치.2충방법측정치비치적평균치C/K=1.023.종상 ,두마사연소법가이작위인삼단백질함량적상규검측방법.
The paper selected 14 ginseng samples and 2 standard reference materials as the measure and study objects ,determined and analyzed the protein content of these tested samples by Kjeldahl and combustion methods ,respectively.The results showed that no significant differences were found in the quantitative results( P>0.05).With the Kjeldahl determination ,the average coefficient of variation (CV) was 2.010% and the CV was 1.156% which produced with the combustion method ,less than 5%.The protein content of most testing samples measured with the combustion method was higher than those with the Kjeldahl method.The average ratio of measured results which obtained by above two methods was 1.023.It was concluded that combustion method can be used as a regular method for determination of protein contents in ginseng.