CAJ | 학술논문

目的:研究体外培养髁突骨软骨瘤细胞的生物学行为. 方法:体外培养人髁突骨软骨瘤细胞,应用HE染色观察细胞形态,吉姆萨染色检测其克隆形成能力,阿利新蓝染色、逆转录聚合酶链反应和免疫荧光染色检测其软骨标记物表达. 结果:髁突骨软骨瘤细胞能在体外成功培养和传代. 细胞呈梭形、星形或多角形, 细胞核有核异型性. 细胞有较强的克隆形成能力,克隆形成率为16%±1.2%. 髁突骨软骨瘤细胞能分泌糖胺聚糖,表达Col2和Sox9. 结论:髁突骨软骨瘤细胞具有肿瘤细胞和软骨细胞的双重特性,为研究髁突骨软骨瘤的防治提供了一个细胞模型.
목적:연구체외배양과돌골연골류세포적생물학행위. 방법:체외배양인과돌골연골류세포,응용HE염색관찰세포형태,길모살염색검측기극륭형성능력,아리신람염색、역전록취합매련반응화면역형광염색검측기연골표기물표체. 결과:과돌골연골류세포능재체외성공배양화전대. 세포정사형、성형혹다각형, 세포핵유핵이형성. 세포유교강적극륭형성능력,극륭형성솔위16%±1.2%. 과돌골연골류세포능분비당알취당,표체Col2화Sox9. 결론:과돌골연골류세포구유종류세포화연골세포적쌍중특성,위연구과돌골연골류적방치제공료일개세포모형.
Objective:To investigate the biological characteristics of cultured mandibular condyle osteochondroma cells in vitro. Methods: The human osteochondroma cells of mandibular condyle were harvested from 3 patients who provided informed consents, and cultured in vitro. The cellular morphology was investigated based on HE staining. The clone for-mation was observed by the Giemsa staining, and the biologic synthesis of the chondrocytes was determined by Alcian Blue staining, Col2 and Sox9 immunofluorescent staining and RT-PCR detection. Results: The condylar osteochondroma cells was successfully cultured and passaged in vitro. Most osteochondroma cells presented as spindle and polygonal shape, and showed heteromorphic nucleus. The condylar osteochondroma cells exhibited high capacity of colony formation, and the colony-forming efficiency was 16%±1.2%. The condylar osteochondroma cells also expressed Col 2, Sox 9 and glycosaminoglycans. Conclusion:The osteochondroma cells of mandibular condyle exhibited the characteristics of both the tumor cells and normal chondrocytes.