CAJ | 학술논문

目的:探讨姜黄素对口腔鳞癌SCC-25细胞EGFR信号通路表达的影响及其相关的分子机制. 方法:不同浓度姜黄素作用细胞24 h,MTT法检测姜黄素对细胞增殖作用的影响,Transwell实验检测EGF 和姜黄素共同作用下细胞侵袭能力的变化,Western blot检测姜黄素对SCC-25细胞EGFR活性及下游信号分子 (Akt、ERK1/2 和 STAT3 )表达的影响. 结果:不同浓度姜黄素作用SCC-25细胞24 h后,均可抑制SCC-25细胞增殖,亦可以抑制EGF诱导的细胞侵袭转移.Western blot结果显示姜黄素对SCC-25细胞总EGFR表达无影响,但可以剂量依赖性抑制SCC-25细胞p-EGFR表达,同时可以剂量依赖性抑制EGFR信号通路下游转录因子Akt、ERK1/2 和 STAT3磷酸化. 结论:姜黄素可剂量依赖性抑制SCC-25细胞EGFR磷酸化活性及其下游信号通路分子(Akt、ERK1/2、STAT3)的磷酸化.
목적:탐토강황소대구강린암SCC-25세포EGFR신호통로표체적영향급기상관적분자궤제. 방법:불동농도강황소작용세포24 h,MTT법검측강황소대세포증식작용적영향,Transwell실험검측EGF 화강황소공동작용하세포침습능력적변화,Western blot검측강황소대SCC-25세포EGFR활성급하유신호분자 (Akt、ERK1/2 화 STAT3 )표체적영향. 결과:불동농도강황소작용SCC-25세포24 h후,균가억제SCC-25세포증식,역가이억제EGF유도적세포침습전이.Western blot결과현시강황소대SCC-25세포총EGFR표체무영향,단가이제량의뢰성억제SCC-25세포p-EGFR표체,동시가이제량의뢰성억제EGFR신호통로하유전록인자Akt、ERK1/2 화 STAT3린산화. 결론:강황소가제량의뢰성억제SCC-25세포EGFR린산화활성급기하유신호통로분자(Akt、ERK1/2、STAT3)적린산화.
Objective:To study whether Curcumin was associated with the signaling pathway expression of EGFR of hu-man oral squamous cell carcinoma (OSCC) SCC-25 cells and its molecular mechanisms. Methods:The efficacy of curcum-in on proliferation in SCC-25 cell line were investigated. The inhibition effect of curcumin on EGF-induced SCC-25 cells invasion were explored by transwell assay. The efficacy of curcumin on the activition of EGFR and its downstream sig-naling molecules Akt, ERK1/2 and STAT3 were investigated by western blot. Results: Curcumin inhibited SCC-25 cells proliferation and EGF-triggered SCC-25 cells invasion. Curcumin regulated the p-EGFR and EGFR downstream signal-ing molecules including Akt, ERK1/2 and STAT3. Conclusion: Curcumin reduced SCC-25 cells proliferation and inva-sion through inhibiting the phosphorylation of EGFR and EGFR downstream signaling molecules Akt, ERK1/2 and STAT3.