CAJ | 학술논문

目的探讨大鼠急性肺损伤声像图与组织病理严重程度的相关性.方法 20只雄性Wistar大鼠随机分为对照组(4只)和实验组(16只),用油酸和脂多糖分两个阶段诱导建立大鼠急性肺损伤模型,分别于两个阶段造模后行肺部超声检查,进行声像图量化评分;处死大鼠留取肺标本进行组织病理观察并评分,显微镜(200倍)下行肺泡计数.结果随着肺损伤的加重,肺声像图表现为胸膜线显示不清或消失、B-线密集分布、肺实变伴支气管充气征等改变.对照组声像图评分为(0.25±0.50)分,肺损伤病理评分为(0.50±0.58)分,肺泡数(25±3)个;油酸组则分别为(2.86±1.35)分,(6.28±0.76)分,(10±2)个;油酸+脂多糖组则分别为(5.83±2.32)分,(9.83±0.98)分,(6±2)个.声像图评分、病理评分与肺泡计数在三组间比较差异均有统计学意义(P＜0.01).结论大鼠急性肺损伤声像图与组织病理严重程度呈正相关,声像图能有效地评价大鼠急性肺损伤严重度.
목적 탐토대서급성폐손상성상도여조직병리엄중정도적상관성.방법 20지웅성Wistar대서수궤분위대조조(4지)화실험조(16지),용유산화지다당분량개계단유도건립대서급성폐손상모형,분별우량개계단조모후행폐부초성검사,진행성상도양화평분;처사대서류취폐표본진행조직병리관찰병평분,현미경(200배)하행폐포계수.결과 수착폐손상적가중,폐성상도표현위흉막선현시불청혹소실、B-선밀집분포、폐실변반지기관충기정등개변.대조조성상도평분위(0.25±0.50)분,폐손상병리평분위(0.50±0.58)분,폐포수(25±3)개;유산조칙분별위(2.86±1.35)분,(6.28±0.76)분,(10±2)개;유산+지다당조칙분별위(5.83±2.32)분,(9.83±0.98)분,(6±2)개.성상도평분、병리평분여폐포계수재삼조간비교차이균유통계학의의(P＜0.01).결론 대서급성폐손상성상도여조직병리엄중정도정정상관,성상도능유효지평개대서급성폐손상엄중도.
Objective To evaluate the correlation between ultrasonography and histopathology in acute lung injury of rats.Methods Twenty male Wistar rats were randomly divided into control group (4 rats) and experimental group (16 rats),in the experimental group acute lung injury models were step-by-step induced by oleic acid (OA) and sequentially by OA and lipopolysaccharide (LPS).After the end of each step of models,ultrasound examination was applied,and the rats were sacrificed to take the lung specimens to be observed.The ultrasonography and pathological results were analyzed,and the numbers of alveolar were counted under microscope (200 times).Results With the aggravation of acute lung injury,the sonographic findings showed unclear or disappeared pleural line,close-set of B-lines,and pulmonary consolidation with air bronchogram.For control group,the ultrasonographic score was 0.25 ± 0.50,lung injury pathological score was 0.50 ± 0.58,and the numbers of alveolar were 25 ± 3.For OA group,the ultrasonographic score was 2.86-± 1.35,lung injury pathological score was 6.28-± 0.76,and the numbers of alveolar were 10-± 2.For OA + LPS group,the ultrasonographic score was 5.83-± 2.32,lung injury pathological score was 9.83 ± 0.98,and the numbers of alveolar were 6-± 2.All 3 groups were significant different on ultrasonographic score,pathological score and alveolar counts (P ＜ 0.01).Conclusions The ultrasonography image of acute lung injury of rats were positively correlated with its histopathological alteration.The ultrasonography can effectively evaluate the degree of acute lung injury of rats.