CAJ | 학술논문

建立排除黄芪片剂中辅料干扰多糖含量测定的方法.采用苯酚-硫酸比色法测定黄芪多糖含量,糖化酶水解麦芽糊精,80%乙醇溶液进行醇沉分离,考察反应温度、pH、酶与底物比例及反应时间对酶解反应的影响,验证糖化酶能否水解黄芪多糖.反应温度58℃、pH4.5、酶与底物比例1:5、反应时间28 min时麦芽糊精完全水解,80%醇沉后在490 nm处测定无明显吸收. 采用酶解法可以准确测定黄芪片剂中多糖的含量, 测定结果与加入量无差异(P>0.05).糖化酶水解麦芽糊精,不水解黄芪多糖,对黄芪多糖含量的测定不存在干扰,可用于片剂中多糖含量测定.
건립배제황기편제중보료간우다당함량측정적방법.채용분분-류산비색법측정황기다당함량,당화매수해맥아호정,80%을순용액진행순침분리,고찰반응온도、pH、매여저물비례급반응시간대매해반응적영향,험증당화매능부수해황기다당.반응온도58℃、pH4.5、매여저물비례1:5、반응시간28 min시맥아호정완전수해,80%순침후재490 nm처측정무명현흡수. 채용매해법가이준학측정황기편제중다당적함량, 측정결과여가입량무차이(P>0.05).당화매수해맥아호정,불수해황기다당,대황기다당함량적측정불존재간우,가용우편제중다당함량측정.
To develop a method for excludeing the interfere of adjuvant on determination of polysaccharide in tablets. A phenol sulfuric acid method was used to determine the content of polysaccharide. Maltodextrin was hydrolyzed with saccharifying enzyme, precipitatede with 80 % ethanol. The effects of temperature, pH, enzyme dosage and reaction time on the hydrolysis were investigated. Morever , the hydrolysis effect of saccharifying enzyme on Astragalus polysaccharide was inspected. Results showed that saccharifying enzyme could fully hydrolyze maltodextrin at the condition of :temperature of 58℃, pH4.5, enzyme to substrate ratio was 1:5, reaction time 28 min, as well as not hydrolyze Astragalus polysaccharides. The saccharifying enzyme could excluded the interference of maltodextrin on determination of polysaccharide, the method was simple, accurate, reproducible, and could be used for determination of Astragalus polysaccharides in buccal tablets.