CAJ | 학술논문

目的利用恒温电热仪建立大鼠烫伤痛模型.方法清洁级健康雄性SD大鼠36只,体重200～ 250 g,采用随机数字表法分为4组(n=9):对照组(C组)、烫伤5 s组(S5组)、烫伤10s组(S10组)和烫伤15 s组(S15组).S5组、S10组和S15组分别接触恒温电热仪热板(85℃)5、10、15s,C组接触热板(室温)10s.分别于处理前1 d(T0)、处理后1、3、5、7和14 d(T1-5)时测定机械痛阈和热痛阈,于处理即刻及处理后24 h时观察并记录大鼠的全身状况、创面色泽及边缘形状,于处理后24 h时随机处死3只大鼠,取足底皮肤行病理学检查.结果与C组比较,S5组T1-2时热痛阈、T1-3时机械痛阈降低,S10组T1-4时热痛阈和机械痛阈降低(P＜0.05).S15组T1-5时热痛阈＞25 s,机械痛阈＞30 g.S10组足部肿胀明显,烫伤部位水疱形成,损伤程度较S5组和S15组加重.结论利用恒温电热仪85℃烫伤10s成功建立大鼠烫伤痛模型.
목적 이용항온전열의건립대서탕상통모형.방법 청길급건강웅성SD대서36지,체중200～ 250 g,채용수궤수자표법분위4조(n=9):대조조(C조)、탕상5 s조(S5조)、탕상10s조(S10조)화탕상15 s조(S15조).S5조、S10조화S15조분별접촉항온전열의열판(85℃)5、10、15s,C조접촉열판(실온)10s.분별우처리전1 d(T0)、처리후1、3、5、7화14 d(T1-5)시측정궤계통역화열통역,우처리즉각급처리후24 h시관찰병기록대서적전신상황、창면색택급변연형상,우처리후24 h시수궤처사3지대서,취족저피부행병이학검사.결과 여C조비교,S5조T1-2시열통역、T1-3시궤계통역강저,S10조T1-4시열통역화궤계통역강저(P＜0.05).S15조T1-5시열통역＞25 s,궤계통역＞30 g.S10조족부종창명현,탕상부위수포형성,손상정도교S5조화S15조가중.결론 이용항온전열의85℃탕상10s성공건립대서탕상통모형.
Objective To establish a scald-induced pain model using a constant-temperature electrical scald instrument in rats.Methods Thirty-six pathogen-free male Sprague-Dawley rats, weighing 200-250 g, were randomly divided into 4 groups (n =9 each) using a random number table: control group (group C), scald for 5 s group (group S5), scald for 10 s group (group S10), and scald for 15 s group (group S15).The rats were anesthetized with intraperitoneal chloral hydrate.In S5, S10 and S15 groups, the plantar surface of the left hindpaw of rats were exposed to a constant-temperature electrical scald instrument (85 ℃) for 5, 10 and 15 s, respectively.The plantar surface of the left hindpaw of rats was exposed to an electrical scald instrument (room temperature) for 10 s in group C.At 1 day before treatment (T0),and 1, 3, 5, 7 and 14 days after treatment (T1-5), the mechanical and thermal pain thresholds were measured.Immediately after treatment, and at 24 h after treatment, the total body condition, wound color, and shape of the margin of the wound were observed and recorded.At 24 h after treatment, 3 rats were randomly sacrificed, and the skin from the plantar surface of the left hindpaw was removed for microscopic examination.Results Compared with group C, the thermal pain threshold at T1-2, and the mechanical pain threshold at T1-3 were significantly decreased in group S5, and the thermal pain threshold,and mechanical pain threshold were significantly decreased at T1-4 in group S10 (P＜0.05).The thermal pain threshold ＞ 25 s, and the mechanical pain threshold ＞30 g at T1-5 in group S15.The swelling in foot was bovious, burn blister appeared, and the degree of damage was aggravated in group S10 compared with S5 and S15 groups.Conclusion The scald-induced pain model is successfully established using a constant-temperature electrical scald instrument in rats.