中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
Chinese Journal of Anesthesiology
2015年
9期
1075-1078
,共4页
徐龙生%冯勤丽%张小平%姚明
徐龍生%馮勤麗%張小平%姚明
서룡생%풍근려%장소평%요명
炎症%疼痛%弓状核%受体,N-甲基-D-天冬氨酸
炎癥%疼痛%弓狀覈%受體,N-甲基-D-天鼕氨痠
염증%동통%궁상핵%수체,N-갑기-D-천동안산
Inflammation%Pain%Arcuate nucleus%Receptors,N-methyl-D-aspartate
目的 探讨弓状核含2B亚基的NMDA受体(NR2B)在大鼠炎性痛形成中的作用.方法 雄性SD大鼠108只,体重200～ 250 g,9周龄,采用随机数字表法,将其分为4组:假手术组(S组,n=47)、炎性痛组(IP组,n=47)、二甲基亚砜对照组(DMSO组,n=7)和NR2B拮抗剂Ro25-6981组(Ro25-6981组,n=7).采用大鼠左侧后肢足垫皮内注射完全弗氏佐剂(CFA)0.1 ml的方法制备炎性痛模型.Ro25-6981组于造模后第3天弓状核内注射Ro25-6981 400 pmol.取7只大鼠,分别于CFA注射前1 d(T1)、注射后第2天(T2)、第3天给药前30 min (T3)、第3天给药后30 min(T4)和第5天(T5)时测定机械缩足反应阈(MWT)和热缩足潜伏期(TWL).S组和IP组于T11-3、T5时处死大鼠,取弓状核组织,采用RT-PCR法测定NR2B mRNA的表达水平,采用Western blot法检测NR2B、磷酸化NR2B(p-NR2B)的表达水平.结果 与S组比较,IP组、DMSO组和Ro25-6981组T2-5时MWT降低,TWL缩短,IP组各时点p-NR2B表达上调(P＜0.05),NR2B及其mRNA表达差异无统计学意义(P＞0.05);与IP组比较,Ro25-6981组T4时MWT升高,TWL延长(P＜0.05),DMSO组各时点MWT和TWL差异无统计学意义(P＞0.05).结论 弓状核NR2B的活化参与了大鼠炎性痛的形成.
目的 探討弓狀覈含2B亞基的NMDA受體(NR2B)在大鼠炎性痛形成中的作用.方法 雄性SD大鼠108隻,體重200～ 250 g,9週齡,採用隨機數字錶法,將其分為4組:假手術組(S組,n=47)、炎性痛組(IP組,n=47)、二甲基亞砜對照組(DMSO組,n=7)和NR2B拮抗劑Ro25-6981組(Ro25-6981組,n=7).採用大鼠左側後肢足墊皮內註射完全弗氏佐劑(CFA)0.1 ml的方法製備炎性痛模型.Ro25-6981組于造模後第3天弓狀覈內註射Ro25-6981 400 pmol.取7隻大鼠,分彆于CFA註射前1 d(T1)、註射後第2天(T2)、第3天給藥前30 min (T3)、第3天給藥後30 min(T4)和第5天(T5)時測定機械縮足反應閾(MWT)和熱縮足潛伏期(TWL).S組和IP組于T11-3、T5時處死大鼠,取弓狀覈組織,採用RT-PCR法測定NR2B mRNA的錶達水平,採用Western blot法檢測NR2B、燐痠化NR2B(p-NR2B)的錶達水平.結果 與S組比較,IP組、DMSO組和Ro25-6981組T2-5時MWT降低,TWL縮短,IP組各時點p-NR2B錶達上調(P＜0.05),NR2B及其mRNA錶達差異無統計學意義(P＞0.05);與IP組比較,Ro25-6981組T4時MWT升高,TWL延長(P＜0.05),DMSO組各時點MWT和TWL差異無統計學意義(P＞0.05).結論 弓狀覈NR2B的活化參與瞭大鼠炎性痛的形成.
목적 탐토궁상핵함2B아기적NMDA수체(NR2B)재대서염성통형성중적작용.방법 웅성SD대서108지,체중200～ 250 g,9주령,채용수궤수자표법,장기분위4조:가수술조(S조,n=47)、염성통조(IP조,n=47)、이갑기아풍대조조(DMSO조,n=7)화NR2B길항제Ro25-6981조(Ro25-6981조,n=7).채용대서좌측후지족점피내주사완전불씨좌제(CFA)0.1 ml적방법제비염성통모형.Ro25-6981조우조모후제3천궁상핵내주사Ro25-6981 400 pmol.취7지대서,분별우CFA주사전1 d(T1)、주사후제2천(T2)、제3천급약전30 min (T3)、제3천급약후30 min(T4)화제5천(T5)시측정궤계축족반응역(MWT)화열축족잠복기(TWL).S조화IP조우T11-3、T5시처사대서,취궁상핵조직,채용RT-PCR법측정NR2B mRNA적표체수평,채용Western blot법검측NR2B、린산화NR2B(p-NR2B)적표체수평.결과 여S조비교,IP조、DMSO조화Ro25-6981조T2-5시MWT강저,TWL축단,IP조각시점p-NR2B표체상조(P＜0.05),NR2B급기mRNA표체차이무통계학의의(P＞0.05);여IP조비교,Ro25-6981조T4시MWT승고,TWL연장(P＜0.05),DMSO조각시점MWT화TWL차이무통계학의의(P＞0.05).결론 궁상핵NR2B적활화삼여료대서염성통적형성.
Objective To investigate the role of 2B subunits-containing N-methyl-D-aspartate receptors (NR2B) in the arcuate nucleus in the development of inflammatory pain (IP) in rats.Methods One hundred and eight male Sprague-Dawley rats, aged 9 weeks, weighing 200-250 g, were randomly divided into 4 groups using a random number table: sham operation group (group S, n =47);group IP (n =47);dimethyl sulfoxide control group (group DMSO, n =7);selective NR2B antagonist Ro25-6981 group (group Ro25-6981, n=7).IP was induced by injecting complete Freund's adjuvant (CFA) 0.1 ml into the plantar surface of the left hindpaw.Ro25-6981 400 pmol was injected into the arcuate nucleus at 3 days after CFA injection.Seven rats in each group were selected for measurement of the mechanical paw withdrawal threshold (MWT) and thermal paw withdrawal latency (TWL) at 1 day before CFA injection (T1) and at 2 days after CFA injection (T2), at 30 min before administration on 3rd day (T3) , at 30 min after administration on 3rd day (T4) , and on 5th day (T5).In S and IP groups, The rats were sacrificed at T1-3 and T5 , and the arcuate nucleus of the hypothalamus was removed for determination of NR2B mRNA expression (by real-time reverse transcriptase polymerase chain reaction) and NR2B and phosphorylated NR2B (p-NR2B) expression (by Western blot).Conclusion Compared with group S, the MWT was significantly decreased, and the TWL was shortened at T2-5 in IP, DMSO and Ro25-6981 groups, and the expression of p-NR2B was up-regulated at each time point (P＜0.05) , and no significant change was found in NR2B protein and mRNA expression in group IP (P＞0.05).Compared with group IP, the MWT was significantly increased, and the TWL was prolonged at T4in group Ro25-6981 (P＜0.05) , and no significant change was found in MWT and TWL at each time point in group DMSO (P＞0.05).Conclusion The activation of NR2B in the arcuate nucleus is involved in the development of IP in rats.
