CAJ | 학술논문

目的评价二十二碳六烯酸对大鼠肝缺血再灌注损伤的影响.方法雄性SD大鼠15只,体重250～ 300 g,8～10周龄,采用随机数字表法,将其分为3组(n=5):假手术组(S组)、肝缺血再灌注组(I/R组)和二十二碳六烯酸组(DHA组).采用阻断肝左叶及中叶肝蒂60 min行再灌注的方法建立肝缺血再灌注损伤模型.DHA组于缺血前30 min和再灌注10 min时分别静脉注射二十二碳六烯酸4 mg/kg,S组及I/R组给予等容量溶媒1 ml.于再灌注24 h时,采集下腔静脉血样,测定血清ALT、AST活性和消退素D1浓度;取血后处死大鼠,取肝组织,采用分光光度法测定髓过氧化物酶(MPO)活性,采用实时定量PCR法测定IL-6和TNF-α的mRNA表达;HE染色,光镜下观察肝组织病理学结果.结果与S组比较,I/R组和DHA组血清AST、ALT和消退素D1的水平升高,肝组织MPO活性、IL-6 mRNA和TNF-α mRNA的表达水平升高(P＜0.05);与I/R组比较,DHA组血清消退素D1浓度升高,肝组织MPO活性和TNF-α mRNA表达水平降低(P＜0.05),血清AST和ALT的活性差异无统计学意义(P＞0.05).I/R组和DHA组肝组织病理学结果无明显差异.结论二十二碳六烯酸可减轻大鼠肝缺血再灌注时炎性反应,但是不足以减轻肝损伤.
목적 평개이십이탄륙희산대대서간결혈재관주손상적영향.방법 웅성SD대서15지,체중250～ 300 g,8～10주령,채용수궤수자표법,장기분위3조(n=5):가수술조(S조)、간결혈재관주조(I/R조)화이십이탄륙희산조(DHA조).채용조단간좌협급중협간체60 min행재관주적방법건립간결혈재관주손상모형.DHA조우결혈전30 min화재관주10 min시분별정맥주사이십이탄륙희산4 mg/kg,S조급I/R조급여등용량용매1 ml.우재관주24 h시,채집하강정맥혈양,측정혈청ALT、AST활성화소퇴소D1농도;취혈후처사대서,취간조직,채용분광광도법측정수과양화물매(MPO)활성,채용실시정량PCR법측정IL-6화TNF-α적mRNA표체;HE염색,광경하관찰간조직병이학결과.결과 여S조비교,I/R조화DHA조혈청AST、ALT화소퇴소D1적수평승고,간조직MPO활성、IL-6 mRNA화TNF-α mRNA적표체수평승고(P＜0.05);여I/R조비교,DHA조혈청소퇴소D1농도승고,간조직MPO활성화TNF-α mRNA표체수평강저(P＜0.05),혈청AST화ALT적활성차이무통계학의의(P＞0.05).I/R조화DHA조간조직병이학결과무명현차이.결론 이십이탄륙희산가감경대서간결혈재관주시염성반응,단시불족이감경간손상.
Objective To evaluate the effect of docosahexaenoic acid (DHA) on hepatic ischemia-reperfusion (I/R) injury in rats.Methods Fifteen male Sprague-Dawley rats, aged 8-10 weeks, weighing 250-300 g, were randomly divided into 3 groups (n =5 each) using a random number table: sham operation group (group S), hepatic I/R group (group I/R) , and group DHA.Hepatic I/R was induced by clamping the hepatic pedicle supplying the left and middle lobes of the liver for 60 min, followed by 24 h reperfusion in anesthetized rats.DHA 4 mg/kg was injected intravenously at 30 min before ischemia and 10 min of reperfusion in group DHA.The equal volume of solvent was given instead in S and I/R groups.Blood samples were taken from the inferior vena cava at 24 h of reperfusion for determination of serum alanine aminotransferase (ALT), and aspartate aminotransferase (AST) activities, and resolvin D1 concentrations.The rats were then sacrificed, and the livers were removed for determination of myeloperoxidase (MPO) activity (by spectrophotometry), and interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) mRNA expression (by quantitative real-time reverse transcriptase polymerase chain reaction).The livers were cut into sections which were stained with haematoxylin and eosin, and examined under light microscope.Results Compared to group S, the serum ALT and AST activities, serum resolvin D1 concentrations, and MPO activity, and IL-6 and TNF-α mRNA expression in liver tissues were significantly increased in I/R and DHA groups (P＜0.05).Compared to group Ⅰ/R, the serum resolvin 1D1 concentrations, and MPO activity and TNF-α mRNA expression in liver tissues were significantly decreased (P＜0.05) , and no significant difference was found in the serum ALT and AST activities in group DHA (P＞0.05).There was no significant difference in pathological changes of the liver between group DHA and group I/R.Conclusion DHA can attenuate inflammatory responses during hepatic I/R, but it is not sufficient to mitigate liver injury in rats.