CAJ | 학술논문

目的分析山东省蓬莱与莱州市优势蜱种及蜱中新型布尼亚病毒(SFTSV)的感染现状.方法于2011年4-11月,根据烟台市往年发热伴血小板减少综合征(SFTS)病例疫情发生情况,选取蓬莱和莱州市为研究场所,每个市选取2个高发乡镇,采用随机数字法在每个乡镇抽取3个村,于每月开展调查,共12个村.采用宿主体表捕捉法采集寄生蜱以及人工布旗法采集游离蜱,共收集蜱3 145只.按蜱来源、种类、发育期分类,研磨后合成样品2 044份,对其提取RNA,检测病毒核酸,巢式PCR扩增S片段,并进行病毒分离.采用邻位相连法绘制系统发育树,采用最小感染率(MIR)以表示蜱中新型布尼亚病毒的感染现状.结果 3 145只蜱按种类共分为5类,其中长角血蜱3 048只(96.92％),血红扇头蜱73只(2.32％),微小牛蜱10只(0.32％),铃头血蜱9只(0.29％)和中华革蜱5只(0.16％).2044份样品中,核酸阳性样品为126份(6.16％),MIR为4.01％,其中长角血蜱为122份(96.83％),血红扇头蜱为3份(2.38％),微小牛蜱为1份(0.79％),MIR分别为4.00％、4.11％和10.00％,铃头血蜱和中华革蜱均无核酸阳性样品.126份核酸阳性样品共扩增出11份S片段全序列,与3株动物来源、2010年烟台市及其他地区分离到的各11株病毒的S片段同源性为95.6％～99.9％,各毒株间无明显的地域特征,且不同动物来源、种类、发育期的病毒间序列非常接近.结论长角血蜱是山东省蓬莱与莱州市绝对优势蜱种,其与血红扇头蜱和微小牛蜱均可能作为SFTSV的传播媒介,蜱SFTSV的S片段序列与病例血清分离的病毒和动物来源S片段高度同源.
목적 분석산동성봉래여래주시우세비충급비중신형포니아병독(SFTSV)적감염현상.방법 우2011년4-11월,근거연태시왕년발열반혈소판감소종합정(SFTS)병례역정발생정황,선취봉래화래주시위연구장소,매개시선취2개고발향진,채용수궤수자법재매개향진추취3개촌,우매월개전조사,공12개촌.채용숙주체표포착법채집기생비이급인공포기법채집유리비,공수집비3 145지.안비래원、충류、발육기분류,연마후합성양품2 044빈,대기제취RNA,검측병독핵산,소식PCR확증S편단,병진행병독분리.채용린위상련법회제계통발육수,채용최소감염솔(MIR)이표시비중신형포니아병독적감염현상.결과 3 145지비안충류공분위5류,기중장각혈비3 048지(96.92％),혈홍선두비73지(2.32％),미소우비10지(0.32％),령두혈비9지(0.29％)화중화혁비5지(0.16％).2044빈양품중,핵산양성양품위126빈(6.16％),MIR위4.01％,기중장각혈비위122빈(96.83％),혈홍선두비위3빈(2.38％),미소우비위1빈(0.79％),MIR분별위4.00％、4.11％화10.00％,령두혈비화중화혁비균무핵산양성양품.126빈핵산양성양품공확증출11빈S편단전서렬,여3주동물래원、2010년연태시급기타지구분리도적각11주병독적S편단동원성위95.6％～99.9％,각독주간무명현적지역특정,차불동동물래원、충류、발육기적병독간서렬비상접근.결론 장각혈비시산동성봉래여래주시절대우세비충,기여혈홍선두비화미소우비균가능작위SFTSV적전파매개,비SFTSV적S편단서렬여병례혈청분리적병독화동물래원S편단고도동원.
Objective To investigate the predominance ticks and the infectious status of severe fever with thrombocytopenia (SFTSV) in Penglai and Laizhou counties, Shandong province.Methods Two towns with high incidence rate were selected in Penglai and Laizhou, respectively, then three villages were selected in each towns.Parasitic ticks were collected from the host skin by hand manually and free ticks manually with white cloth from the grassland, monthly, during April to December in 2011.Samples were classified by original, varieties, developmental stages, then extracted RNA, using Realtime RT-PCR to test severe fever thrombocytopenia syndrome virus, S fragments were amplified with nested PCR, then isolated virus.By neighbor joining method in the phylogenetic tree, the minimum infection rate (MIR) was used to represent the infection status of ticks in novel bunyavirus.Results A total of 3 145 ticks were collected totally from 5 categories, there were 3 048(96.92％) of Haemaphysalis longicornis, 73(2.32％) of Rhinpicephalus sanguineus, 10(0.32％) of microplus Boophilus, 9(0.29％) of Haemaphysalis campanulata, 5 (0.16％) of Dermacentor sinicus, respectively.The positive rate of nucleic acid of 2 044 samples was 6.16％ (126/2 044), minimum infection rate (MIR) was 4.01％, there were 122(96.83％) of Haemaphysalis longicornis, 3(2.38％) of Rhinpicephalus sanguineus, and 1 (0.79％) of microplus Boophilus, MIR was 4.00％, 4.11％, and 10.00％, respectively.There were no nucleic acid positive samples in Haemaphysalis campanulata and Dermacentor sinicus.The 11 S segments were amplified in 126 positive samples, the homology of S fragment was 95.6％-99.9％ with 11 strains isolated from the identified SFTS cases in local area, 3 strains isolated from animals, and 11 strains isolated from other areas.There was no significant difference among original, varieties and developmental stages.Conclusion Haemaphysalis longicornis was the predominant species in Penglai and Laizhou counties, it could be propagation medium with Rhipicephalus sanguineus and microplus Boophilus, S sequence in ticks was higher homology with virus isolated from local SFTS cases.