CAJ | 학술논문

目的探讨腺病毒介导睫状神经营养因子(CNTF)基因转染的人脐血干细胞(hUCBCs)复合壳聚糖支架(CNTF-hUCBCs复合壳聚糖支架)移植对颅内段视神经损伤的修复作用. 方法 80只Wistar大鼠按随机数字表法分为正常对照组、视神经损伤组、视神经损伤溶剂对照组、hUCBCs移植组、CNTF-hUCBCs复合壳聚糖支架移植组,每组各16只.后4组模拟额下显微外科入路建立颅内段视神经损伤模型,后3组分别在模型鼠眼玻璃体内给予生理盐水注射、hUCBCs移植及CNTF-hUCBCs复合壳聚糖支架移植.损伤后2周采用免疫组化染色检测各组大鼠视网膜神经节细胞(RGCs)表达并计数比较. 结果与正常对照组大鼠[(1580.65±84.36)个]比较,视神经损伤组及视神经损伤溶剂对照组每视野下视网膜平均标记RCCs数[(351.38±29.73)个、(382.05±47.22)个]明显减少,差异均有统计学意义(P＜0.05);与视神经损伤组及视神经损伤溶剂对照组比较,hUCBCs移植组及CNTF-hUCBCs复合壳聚糖支架移植组每视野下视网膜平均标记RCCs数[(614.82±37.21)个、(937.59±62.25)个]明显增多,差异均有统计学意义(P＜0.05);hUCBCs移植组与CNTF-hUCBCs复合壳聚糖支架移植组比较差异亦有统计学意义(P＜0.05). 结论 CNTF-hUCBCs复合壳聚糖支架移植较单纯hUCBCs移植对大鼠颅内段视神经损伤的修复作用更显著.
목적 탐토선병독개도첩상신경영양인자(CNTF)기인전염적인제혈간세포(hUCBCs)복합각취당지가(CNTF-hUCBCs복합각취당지가)이식대로내단시신경손상적수복작용. 방법 80지Wistar대서안수궤수자표법분위정상대조조、시신경손상조、시신경손상용제대조조、hUCBCs이식조、CNTF-hUCBCs복합각취당지가이식조,매조각16지.후4조모의액하현미외과입로건립로내단시신경손상모형,후3조분별재모형서안파리체내급여생리염수주사、hUCBCs이식급CNTF-hUCBCs복합각취당지가이식.손상후2주채용면역조화염색검측각조대서시망막신경절세포(RGCs)표체병계수비교. 결과 여정상대조조대서[(1580.65±84.36)개]비교,시신경손상조급시신경손상용제대조조매시야하시망막평균표기RCCs수[(351.38±29.73)개、(382.05±47.22)개]명현감소,차이균유통계학의의(P＜0.05);여시신경손상조급시신경손상용제대조조비교,hUCBCs이식조급CNTF-hUCBCs복합각취당지가이식조매시야하시망막평균표기RCCs수[(614.82±37.21)개、(937.59±62.25)개]명현증다,차이균유통계학의의(P＜0.05);hUCBCs이식조여CNTF-hUCBCs복합각취당지가이식조비교차이역유통계학의의(P＜0.05). 결론 CNTF-hUCBCs복합각취당지가이식교단순hUCBCs이식대대서로내단시신경손상적수복작용경현저.
Objective To investigate the recovery effect of intraocular transplantation of chitosan scaffold composited by ciliary neurotrophic factor (CNTF) transfected human umbilical cord blood stem cells (hUCBCs) on intracranial optic nerve injury (IONI) in rats.Methods Eighty Wistar rats were randomly divided into five groups (n=16):normal control group,IONI model group,IONI+vehicle group,IONI+hUCBCs group and IONI+CNTF-hUCBCs-chitosan scaffold group;IONI models in the later four groups were established by microsurgery through subfrontal approach;and vitreous tissues in the rats of the later three groups were given saline injection,and hUCBCs and CNTF-hUCBCs-chitosan scaffold transplantation,respectively.Immunohistochemical method was employed to detect retinal ganglion cells (RGCs) expression and the number of the cells.Results As compared with the normal control group (1580.65±84.36),the IONI model group and IONI+vehicle group had significantly decreased number of RGCs (351.38±29.73 and 382.05±47.22,P＜0.05);as compared with the IONI model group and IONI+vehicle group,IONI+hUCBCs group and IONI+CNTF-hUCBCs-chitosan scaffold group had significantly increased number of RGCs (614.82±37.21 and 937.59±62.25,P＜0.05);and the difference between the IONI+hUCBCs group and IONI+CNTF-hUCBCs-chitosan scaffold group was significant (P＜0.05).Conclusion There is more significant repair effect of intraocular transplantation of chitosan scaffold composited by CNTF-transfected hUCBCs than pure hUCBCs transplantation on rats after IONI.