CAJ | 학술논문

目的探讨联合服用色氨酸对氟西汀治疗抑郁模型大鼠抑郁、焦虑样行为的影响.方法将60只雄性Wistar大鼠按随机区组法分为对照组、色氨酸组、氟西汀组和色氨酸+氟西汀组,各15只.对照组大鼠给予9g/L氯化钠注射液灌胃(5 mL/kg),色氨酸组给予50 mg/kg色氨酸,氟西汀组给予10 mg/kg氟西汀,色氨酸+氟西汀组同时给予大鼠50 mg/kg色氨酸和10 mg/kg氟西汀灌胃.4组大鼠均给予21d长期慢性、中等和不可预知应激刺激建立抑郁模型.采用改良强迫游泳实验检测4组大鼠静止不动时间、游泳时间及攀爬时间.采用高架十字迷宫实验检测4组大鼠进入开放臂、闭合臂时间,进入中央区时间及进入开放臂次数占总次数百分比.高效液相色谱法测定4组大鼠脑脊液中色氨酸水平. 结果 (1)4组大鼠静止不动时间、游泳时间、攀爬时间差异均有统计学意义(P＜0.05).氟西汀和色氨酸均对大鼠改良强迫游泳实验行为学指标有显著影响.(2)4组大鼠进入开放臂时间、进入闭合臂和中央区的时间差异均有统计学意义(P＜0.05).氟西汀对大鼠高架十字迷宫实验行为学指标有显著影响.色氨酸对大鼠进入各臂与中央区的次数、时间无显著影响.氟西汀和色氨酸联用对大鼠高架十字迷宫实验行为学指标的影响不存在显著交互作用.(3)4组大鼠脑脊液中色氨酸水平测定结果差异有统计学意义(F=6.874,P=0.002).色氨酸+氟西汀组大鼠脑脊液中色氨酸水平显著高于对照组及色氨酸组,差异均有统计学意义(P＜0.05). 结论 (1)色氨酸可加重大鼠抑郁样行为,对焦虑行为无明显作用;(2)氟西汀可逆转大鼠抑郁样行为,但致焦虑;(3)色氨酸和氟西汀联合使用对治疗大鼠焦虑样行为、抑郁样行为无明显效果.
목적 탐토연합복용색안산대불서정치료억욱모형대서억욱、초필양행위적영향.방법 장60지웅성Wistar대서안수궤구조법분위대조조、색안산조、불서정조화색안산+불서정조,각15지.대조조대서급여9g/L록화납주사액관위(5 mL/kg),색안산조급여50 mg/kg색안산,불서정조급여10 mg/kg불서정,색안산+불서정조동시급여대서50 mg/kg색안산화10 mg/kg불서정관위.4조대서균급여21d장기만성、중등화불가예지응격자격건립억욱모형.채용개량강박유영실험검측4조대서정지불동시간、유영시간급반파시간.채용고가십자미궁실험검측4조대서진입개방비、폐합비시간,진입중앙구시간급진입개방비차수점총차수백분비.고효액상색보법측정4조대서뇌척액중색안산수평. 결과 (1)4조대서정지불동시간、유영시간、반파시간차이균유통계학의의(P＜0.05).불서정화색안산균대대서개량강박유영실험행위학지표유현저영향.(2)4조대서진입개방비시간、진입폐합비화중앙구적시간차이균유통계학의의(P＜0.05).불서정대대서고가십자미궁실험행위학지표유현저영향.색안산대대서진입각비여중앙구적차수、시간무현저영향.불서정화색안산련용대대서고가십자미궁실험행위학지표적영향불존재현저교호작용.(3)4조대서뇌척액중색안산수평측정결과차이유통계학의의(F=6.874,P=0.002).색안산+불서정조대서뇌척액중색안산수평현저고우대조조급색안산조,차이균유통계학의의(P＜0.05). 결론 (1)색안산가가중대서억욱양행위,대초필행위무명현작용;(2)불서정가역전대서억욱양행위,단치초필;(3)색안산화불서정연합사용대치료대서초필양행위、억욱양행위무명현효과.
Objective To investigate the effects of concomitant administration oftryptophan on depression-related and anxiety-like behaviors of fluoxetine treated adult rats.Methods Sixty male Wistar rats were divided into the following groups by randomized block design:control group,L-tryptophan group,fluoxetine group,and experimental group (n=15);9 g/L sodium chloride injection (5 ml/kg),50 mg/kg L-tryptophan,10 mg/kg fluoxetine and both 50 mg/kg L-tryptophan and 10 mg/kg fluoxetine were,respectively,given to the rats in the above four groups via intragastric administration;depression models were established by 21 d-long-term chronic,medium and unpredictable stress stimulation.The modified forced swimming test (FST) was used to detect the times spent in immobility behavior,swimming behavior and climbing behavior;and elevated plus-maze (EPM) was employed to detect the times spent in open arms,closed arms and center area,and ratio of entries into open arms to the total entries;while tryptophan levels in cerebrospinal fluid were measured by high performance liquid chromatography.Results The times spent in immobility behavior,swimming behavior and climbing behavior showed significant differences among the four groups (P＜0.05);both fluoxetine and tryptophan had significant influences in FST results.The times spent in open arms,closed arms and center area showed significant differences among the four groups (P＜0.05);fluoxetine had significant influences in EPM results,and tryptophan had no significant influences in times spent in open arms,closed arms and center area;moreover,there was no significant interaction between tryptophan and fluoxetine treatments in parameters of FST and EPM.Significant difference of tryptophan level was noted among the four groups (F=6.874,P=0.002);that in the experimental group was significantly higher than that in the fluoxetine and control group (P＜0.05).Conclusions Tryptophan can increase depressive-related behavior,but not alter anxiety-like behavior.Fluoxetine can decrease depression-related behavior,but induce anxiety.Concomitant use oftryptophan and fluoxetine does not alter anxiety-like behavior and the antidepressant effect offluoxetine is not enhanced.