中华内分泌代谢杂志
中華內分泌代謝雜誌
중화내분비대사잡지
Chinese Journal of Endocrinology and Metabolism
2015年
11期
982-987
,共6页
元荣荣%朱丽萍%李丹丹%高威%薛磊%庞妩燕%赵志刚
元榮榮%硃麗萍%李丹丹%高威%薛磊%龐嫵燕%趙誌剛
원영영%주려평%리단단%고위%설뢰%방무연%조지강
内皮祖细胞%糖尿病大鼠%周围神经病变
內皮祖細胞%糖尿病大鼠%週圍神經病變
내피조세포%당뇨병대서%주위신경병변
Endothelial progenitor cells%Diabetic rats%Peripheral neuropathy
目的 观察大鼠骨髓内皮祖细胞(bone marrow-derived endothelial progenitor cells, EPCs)移植是否对糖尿病大鼠坐骨神经病变有治疗作用.方法 用链脲佐菌素60 mg/kg腹腔注射制备糖尿病大鼠.将雄性SD大鼠分为正常组(NC组)、糖尿病周围神经病变(diabetic peripheral neuropathy,DPN)组(DPN组)、DPN+生理盐水组(DPN+S组)和DPN+EPCs组.用肌电图与诱发电位仪测定坐骨神经运动神经传导速度(motor nerve conduction velocity,MNCV),在光镜和电镜下观察坐骨神经病理学改变.结果 与NC组[(52.91 ±4.53) m/s]相比,DPN组、DPN+S组大鼠的坐骨神经MNCV明显减慢[(36.51±6.30对25.37±5.48)m/s,P<0.01].与DPN组相比,DPN+EPCs组的MNCV明显提高[(36.51±6.30对46.20±11.70)rn/s,P<0.01].光镜和电镜观察发现:DPN组、DPN+EPCs组和DPN+S组坐骨神经均有脱髓鞘及轴索萎缩等病理改变;与DPN+S组相比,DPN+EPCs组的病变程度较轻.结论 糖尿病大鼠成模12周时能成功构建DPN大鼠.EPCs移植能改善DPN大鼠的坐骨神经MNCV、脱髓鞘及轴索萎缩等病理学改变.
目的 觀察大鼠骨髓內皮祖細胞(bone marrow-derived endothelial progenitor cells, EPCs)移植是否對糖尿病大鼠坐骨神經病變有治療作用.方法 用鏈脲佐菌素60 mg/kg腹腔註射製備糖尿病大鼠.將雄性SD大鼠分為正常組(NC組)、糖尿病週圍神經病變(diabetic peripheral neuropathy,DPN)組(DPN組)、DPN+生理鹽水組(DPN+S組)和DPN+EPCs組.用肌電圖與誘髮電位儀測定坐骨神經運動神經傳導速度(motor nerve conduction velocity,MNCV),在光鏡和電鏡下觀察坐骨神經病理學改變.結果 與NC組[(52.91 ±4.53) m/s]相比,DPN組、DPN+S組大鼠的坐骨神經MNCV明顯減慢[(36.51±6.30對25.37±5.48)m/s,P<0.01].與DPN組相比,DPN+EPCs組的MNCV明顯提高[(36.51±6.30對46.20±11.70)rn/s,P<0.01].光鏡和電鏡觀察髮現:DPN組、DPN+EPCs組和DPN+S組坐骨神經均有脫髓鞘及軸索萎縮等病理改變;與DPN+S組相比,DPN+EPCs組的病變程度較輕.結論 糖尿病大鼠成模12週時能成功構建DPN大鼠.EPCs移植能改善DPN大鼠的坐骨神經MNCV、脫髓鞘及軸索萎縮等病理學改變.
목적 관찰대서골수내피조세포(bone marrow-derived endothelial progenitor cells, EPCs)이식시부대당뇨병대서좌골신경병변유치료작용.방법 용련뇨좌균소60 mg/kg복강주사제비당뇨병대서.장웅성SD대서분위정상조(NC조)、당뇨병주위신경병변(diabetic peripheral neuropathy,DPN)조(DPN조)、DPN+생리염수조(DPN+S조)화DPN+EPCs조.용기전도여유발전위의측정좌골신경운동신경전도속도(motor nerve conduction velocity,MNCV),재광경화전경하관찰좌골신경병이학개변.결과 여NC조[(52.91 ±4.53) m/s]상비,DPN조、DPN+S조대서적좌골신경MNCV명현감만[(36.51±6.30대25.37±5.48)m/s,P<0.01].여DPN조상비,DPN+EPCs조적MNCV명현제고[(36.51±6.30대46.20±11.70)rn/s,P<0.01].광경화전경관찰발현:DPN조、DPN+EPCs조화DPN+S조좌골신경균유탈수초급축색위축등병리개변;여DPN+S조상비,DPN+EPCs조적병변정도교경.결론 당뇨병대서성모12주시능성공구건DPN대서.EPCs이식능개선DPN대서적좌골신경MNCV、탈수초급축색위축등병이학개변.
Objective To explore whether endothelial progenitor cells (EPCs) exerts therapeutic effects on rats with diabetic peripheral neuropathy (DPN).Methods Diabetes was induced by streptozotocin.Male SD rats were divided into normal control group(NC group), diabetic peripheral neuropathy group(DPN group), DPN+saline group(DPN+S group), and DPN+EPCs group.Sciatic nerve motor nerve conduction velocity(MNCV) was measured by a electromyography and trigger potentiometer instrument.Pathological changes were observed under optical microscope and electron microscope.Results Compared with normal control group [(52.91 ± 4.53) m/s], both DPN group and DPN+S group had slower sciatic nerve MNCV [(36.51 ± 6.30 and 25.37 ± 5.48) m/s, P<0.01].Compared with DPN group, the MNCV of sciatic nerve in DPN + EPCs group had improved significantly [(36.51 ± 6.30 vs 46.20 ± 11.70) m/s, P < 0.01].Sciatic nerve pathological changes, characterized by demyelination and axonal degeneration under light microscope and electron microscope were observed in DPN group, DPN+EPCs group, and DPN+S group.Compared with DPN+S group, the sciatic nerve pathological changes of DPN+EPCs group were alleviated.Conclusion After fed for 12 weeks, diabetic rats could progress to DPN rats.Transplantation of EPCs could improve MNCV, demyelination and axonal degeneration changes of sciatic nerver of the DPN rats.