CAJ | 학술논문

目的观察富含半胱氨酸的酸性分泌蛋白(secreted protein acidic and rich in cysteine,SPARC)在2型糖尿病足病患者血清及足部肌肉组织中表达水平的变化.方法选取2型糖尿病未合并下肢动脉血管、周围神经病变患者40例作为DMA组,2型糖尿病合并下肢动脉血管、周围神经病变患者40例作为DMB组,2型糖尿病足病患者40例作为DF组,以及健康体检者(包括血脂异常)40名作为NC组;DF组及NC组各6例取肌肉组织.分别采取ELISA法检测血清SPARC水平,RT-PCR、Western印迹法、免疫荧光法、免疫组化法检测肌肉组织SPARC mRNA和蛋白的表达水平.结果 DMA组及DMB组血清SPARC水平高于NC组及DF组[(1 040.48±212.12、1 068.36±165.45对841.93±144.57、835.43±188.37)ng/L,P＜0.01],DF组与NC组、DMA组与DMB组之间无差异统计学意义(P＞0.05).DF患者足部肌肉组织中SPARC mRNA和蛋白呈高表达(P＜0.01).结论 DF患者足部肌肉组织中SPARC mRNA和蛋白表达增高,表明SPARC可能参与糖尿病足损伤肌肉组织的修复及愈合.
목적 관찰부함반광안산적산성분비단백(secreted protein acidic and rich in cysteine,SPARC)재2형당뇨병족병환자혈청급족부기육조직중표체수평적변화.방법 선취2형당뇨병미합병하지동맥혈관、주위신경병변환자40례작위DMA조,2형당뇨병합병하지동맥혈관、주위신경병변환자40례작위DMB조,2형당뇨병족병환자40례작위DF조,이급건강체검자(포괄혈지이상)40명작위NC조;DF조급NC조각6례취기육조직.분별채취ELISA법검측혈청SPARC수평,RT-PCR、Western인적법、면역형광법、면역조화법검측기육조직SPARC mRNA화단백적표체수평.결과 DMA조급DMB조혈청SPARC수평고우NC조급DF조[(1 040.48±212.12、1 068.36±165.45대841.93±144.57、835.43±188.37)ng/L,P＜0.01],DF조여NC조、DMA조여DMB조지간무차이통계학의의(P＞0.05).DF환자족부기육조직중SPARC mRNA화단백정고표체(P＜0.01).결론 DF환자족부기육조직중SPARC mRNA화단백표체증고,표명SPARC가능삼여당뇨병족손상기육조직적수복급유합.
Objective To observe secreted protein acidic and rich in cysteine (SPARC) levels in serum and foot muscle tissue of patients with type 2 diabetic foot (DF).Methods All participants were divided into four groups with 40 cases in each group, including type 2 diabetic patients without (DMA) and with (DMB) lower limb arterial sclerosis and peripheral neuropathy, DF group, and normal control (NC) group.The muscle tissues of foot from DF group (n =6) and NC group (n =6) were taken.Serum SPARC level was measured with ELISA.RT-PCR, Western blot, immunofluorescence, and immunohistochemistry were used to examine SPARC mRNA and protein expressions in the foot muscle.Results Serum SPARC levels were higher in DMA and DMB groups compared with NC and DF groups[(1 040.48 ±212.12 and 1 068.36 ± 165.45 vs 841.93 ± 144.57 and 835.43 ± 188.37) ng/L, P＜ 0.01].There was no significant difference in serum SPARC level between DF and NC groups or DMA and DMB groups (P＞0.05).The expression levels of SPARC mRNA and protein in the foot muscle were higher in DF compared with NC group (P＜0.05).Conclusion SPARC mRNA and protein expressions in foot muscle tissue are higher in DF group compared with control group, indicating that SPARC may participate in the repair and healing of damaged muscle tissue of diabetic foot.