CAJ | 학술논문

目的建立尿中8种氨基甲酸酯类农药同时测定的方法.方法尿样用乙腈沉淀蛋白后,用液相色谱-串联质谱法进行测定,外标法定量,其中色谱柱采用C18超高效液相色谱柱,流动相为甲醇与乙酸铵溶液梯度淋洗,质谱采集方式为多反应监测.结果样品中氨基甲酸酯类农药的线性范围为20～800 μg/L,当添加浓度为20、200、800 μg/L时,回收率为61.0％～121％,相对标准偏差为1.7％～5.5％.结论该检测方法各项指标均符合GBZT 210.5-2008《职业卫生标准制定指南第5部分:生物材料中化学物质测定方法》的要求,适用于中毒患者尿中8种氨基甲酸酯类农药的同时检测.
목적 건립뇨중8충안기갑산지류농약동시측정적방법.방법 뇨양용을정침정단백후,용액상색보-천련질보법진행측정,외표법정량,기중색보주채용C18초고효액상색보주,류동상위갑순여을산안용액제도림세,질보채집방식위다반응감측.결과 양품중안기갑산지류농약적선성범위위20～800 μg/L,당첨가농도위20、200、800 μg/L시,회수솔위61.0％～121％,상대표준편차위1.7％～5.5％.결론 해검측방법각항지표균부합GBZT 210.5-2008《직업위생표준제정지남제5부분:생물재료중화학물질측정방법》적요구,괄용우중독환자뇨중8충안기갑산지류농약적동시검측.
Objective To establish a method for simultaneously determining the urinary concentrations of 8 carbamate pesticides.Methods After being purified by acetonitrile precipitation,urine samples were transferred to a liquid chromatography-tandem mass spectrometry system,and the concentrations of 8 carbamate pesticides were determined by external standard method.A C18 column was used for ultra-high-performance liquid chromatography;methanol/ammonium acetate solution was used as the mobile phase for gradient elution;the mass spectrometer was operated in a multi-reaction monitoring mode.Results The calibration curves were linear when the urinary concentrations of these carbamate pesticides were 20～800 μg/L,and the recovery rates were 61.0％～121％ at spiked levels of 20,200 and 800 μg/L,with a relative standard deviation of 1.7％～5.5％.Conclusion This determination method meets the Guide for establishing occupational health standards-part 5:Determination methods of chemicals in biological materials,and can be used for simultaneous determination of 8 carbamate pesticides in the urine of poisoning patients.