中华肝胆外科杂志
中華肝膽外科雜誌
중화간담외과잡지
Chinese Journal of Hepatobiliary Surgery
2015年
11期
769-772
,共4页
崔龙海%姜金希%姜国哲%韩春姬
崔龍海%薑金希%薑國哲%韓春姬
최룡해%강금희%강국철%한춘희
轮叶党参皂苷%肝缺血-再灌注%白介素-18%氧化损伤
輪葉黨參皂苷%肝缺血-再灌註%白介素-18%氧化損傷
륜협당삼조감%간결혈-재관주%백개소-18%양화손상
Codonopsis lanceolata saponin%Hepatic ischemia-reperfusion%Interleukin-18%Oxidative damage
目的 探讨轮叶党参总皂苷(CLS)对大鼠肝脏缺血-再灌注损伤(HIRI)的保护作用及其机制.方法 将大鼠随机分为3组,即假手术组(SO)、HIRI组、CLS组,每组10只.采用Pringle法制备大鼠肝HIRI损伤模型.CLS组术前7d开始经口投予CLS200mg·kg-1 ·d-1,SO组和HIRI组给予水10 ml·kg-1·d-1.采用比色法检测血清丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、超氧化物歧化酶(SOD)、丙二醛(MDA)水平以及肝组织SOD和MDA水平,ELISA法测定血清和肝组织中白细胞介素-18(IL-18)含量及血清肿瘤坏死因子(TNF-α)含量.结果 HIRI组血清ALT、AST、MDA、IL-18、TNF-α及肝组织MDA、IL-18水平显著高于SO组(P＜0.05),HIRI组血清和肝组织SOD活性显著低于HIRI组(P ＜0.05);CLS组血清ALT、AST、MDA、IL-18、TNF-α及肝组织MDA、IL-18水平显著低于HIRI组(P＜0.05),血清和肝组织SOD活性显著高于HIRI组(P＜0.05).组织切片显示,HIRI组肝组织有明显的细胞坏死灶及大量炎性细胞浸润,CLS组病变明显轻于HIRI组.结论 轮叶党参皂苷预处理对缺血再灌注肝脏具有明显的保护作用,其机制可能与抑制炎性因子及增强肝脏的抗氧化能力有关.
目的 探討輪葉黨參總皂苷(CLS)對大鼠肝髒缺血-再灌註損傷(HIRI)的保護作用及其機製.方法 將大鼠隨機分為3組,即假手術組(SO)、HIRI組、CLS組,每組10隻.採用Pringle法製備大鼠肝HIRI損傷模型.CLS組術前7d開始經口投予CLS200mg·kg-1 ·d-1,SO組和HIRI組給予水10 ml·kg-1·d-1.採用比色法檢測血清丙氨痠轉氨酶(ALT)、天鼕氨痠轉氨酶(AST)、超氧化物歧化酶(SOD)、丙二醛(MDA)水平以及肝組織SOD和MDA水平,ELISA法測定血清和肝組織中白細胞介素-18(IL-18)含量及血清腫瘤壞死因子(TNF-α)含量.結果 HIRI組血清ALT、AST、MDA、IL-18、TNF-α及肝組織MDA、IL-18水平顯著高于SO組(P＜0.05),HIRI組血清和肝組織SOD活性顯著低于HIRI組(P ＜0.05);CLS組血清ALT、AST、MDA、IL-18、TNF-α及肝組織MDA、IL-18水平顯著低于HIRI組(P＜0.05),血清和肝組織SOD活性顯著高于HIRI組(P＜0.05).組織切片顯示,HIRI組肝組織有明顯的細胞壞死竈及大量炎性細胞浸潤,CLS組病變明顯輕于HIRI組.結論 輪葉黨參皂苷預處理對缺血再灌註肝髒具有明顯的保護作用,其機製可能與抑製炎性因子及增彊肝髒的抗氧化能力有關.
목적 탐토륜협당삼총조감(CLS)대대서간장결혈-재관주손상(HIRI)적보호작용급기궤제.방법 장대서수궤분위3조,즉가수술조(SO)、HIRI조、CLS조,매조10지.채용Pringle법제비대서간HIRI손상모형.CLS조술전7d개시경구투여CLS200mg·kg-1 ·d-1,SO조화HIRI조급여수10 ml·kg-1·d-1.채용비색법검측혈청병안산전안매(ALT)、천동안산전안매(AST)、초양화물기화매(SOD)、병이철(MDA)수평이급간조직SOD화MDA수평,ELISA법측정혈청화간조직중백세포개소-18(IL-18)함량급혈청종류배사인자(TNF-α)함량.결과 HIRI조혈청ALT、AST、MDA、IL-18、TNF-α급간조직MDA、IL-18수평현저고우SO조(P＜0.05),HIRI조혈청화간조직SOD활성현저저우HIRI조(P ＜0.05);CLS조혈청ALT、AST、MDA、IL-18、TNF-α급간조직MDA、IL-18수평현저저우HIRI조(P＜0.05),혈청화간조직SOD활성현저고우HIRI조(P＜0.05).조직절편현시,HIRI조간조직유명현적세포배사조급대량염성세포침윤,CLS조병변명현경우HIRI조.결론 륜협당삼조감예처리대결혈재관주간장구유명현적보호작용,기궤제가능여억제염성인자급증강간장적항양화능력유관.
Objective To investigate the protective effects of Codonopsis lanceolata saponin (CLS) on hepatic ischemia-reperfusion injury (HIRI) and its mechanism in rats.Methods The rats were divided randomly into 3 groups:sham-operated (SO) group, HIRI group and CLS group.HIRI model was made by Pringle method.CLS group was pretreated with CLS (200 mg · kg-1 · d-1) by intragastric administration for 7 days before operation, while SO group and HIRI group were given with distilled water 10 ml · kg-1 · d-1 for 7 days.ALT, AST, SOD, MDA in serum, and SOD and MDA in hepatic tissue were detected by colorimetric method.IL-18 in serum and hepatic tissue was detected by ELISA.Results The levels of serum ALT, AST, MDA, IL-18, TNF-α and tissue MDA, IL-18 in HIRI group were significantly higher than those in SO group and CLS group (all P ＜ 0.05), respectively.The serum and hepatic tissue SOD activity in HIRI group was significantly lower than that in SO group and CLS group (all P ＜ 0.05).Histological observation revealed that the obvious cell necrotic foci with a large number of inflammatory cells infiltration in the HIRI group, while the lesion was obviously alleviated in CLS group.Conclusions Codonopsis lanceolata saponin preconditioning alleviates hepatic ischemia/reperfusion injury.The mechanism may be correlated with inhibiting inflammatory factors and enhancing anti-oxidation.
