CAJ | 학술논문

目的比较三种市售泵用胰岛素对抗等电点沉淀的效果. 方法通过加入稀释盐酸的方法模拟pH值下降过程中,用高效液相色谱(HPLC)测定三种胰岛素(重和林 R、诺和灵 R、优泌林 R)等电点沉淀情况. 并采用高斯拟合的方法绘制出沉淀曲线,并得出三种胰岛素发生沉淀时的pH值. 结果重和林R、诺和灵R和优泌林R在出现10%和90%沉淀时的pH值分别为6. 52和5. 82,6. 85 和6. 00,6. 68 和5. 94. 结论重和林R开始出现沉淀的pH值最低,对抗等电点的作用最强,其次是优泌林R,诺和灵R最差. 胰岛素等电点会引起胰岛素构象改变发生沉淀,阻塞胰岛素泵,进而引起血糖控制不佳. 但本研究仅在体外比较了胰岛素对抗等电点沉淀的不同,与临床中胰岛素泵堵塞之间作用的机制仍需进一步研究.
목적 비교삼충시수빙용이도소대항등전점침정적효과. 방법 통과가입희석염산적방법모의pH치하강과정중,용고효액상색보(HPLC)측정삼충이도소(중화림 R、낙화령 R、우비림 R)등전점침정정황. 병채용고사의합적방법회제출침정곡선,병득출삼충이도소발생침정시적pH치. 결과 중화림R、낙화령R화우비림R재출현10%화90%침정시적pH치분별위6. 52화5. 82,6. 85 화6. 00,6. 68 화5. 94. 결론 중화림R개시출현침정적pH치최저,대항등전점적작용최강,기차시우비림R,낙화령R최차. 이도소등전점회인기이도소구상개변발생침정,조새이도소빙,진이인기혈당공제불가. 단본연구부재체외비교료이도소대항등전점침정적불동,여림상중이도소빙도새지간작용적궤제잉수진일보연구.
Objective To compare the resistance towards isoelectric precipitation of three insulin products used for continuous subcutaneous insulin infusion. Methods The degree of isoelectric insulin precipitation of recombinant human insulin injection( SciLin R) ,Biosynthetic human insulin injection ( Novolin R) ,and recombinant human insulin injection (Humulin R) was examined by HPLC in the simulation of reducing pH with addition of diluted HCl. The modified Gaussian model was used to superimpose the percentage of precipitated insulin versus pH. Results The pH value at the time point of 10% and 90% precipitation occurred was 6. 52 and 5. 82 for SciLin R,6. 85 and 6. 00 for No-volin R,6. 68 and 5. 94 for Humulin R. Conclusion Resistance towards isoelectric precipitation is highest for SciLin R,while the pH value when the precipitation occurred is lowest. The resistance towards isoelectric precipitation of Nov-olin R is lowest. Isoelectric precipitation can alter the pharmacokinetic properties of the insulin and lead to the occlu-sion of the infusion catheter,result in poor control of blood sugar. The article compared the differences in resistance to-wards isoelectric precipitation of different insulin products,and the mechanism in the resistance and the formulation of catheter occlusion requirefurther studies.