中华肝脏病杂志
中華肝髒病雜誌
중화간장병잡지
Chinese Journal of Hepatology
2015年
11期
849-853
,共5页
细胞分化%基因表达%基因芯片
細胞分化%基因錶達%基因芯片
세포분화%기인표체%기인심편
Cell differentiation%Gene expression%Microarray
目的 比较分析人肝脏前体细胞定向肝实质细胞分化过程中基因表达的变化. 方法 分别提取体外培养的人胚肝脏前体细胞及体外诱导分化后具有功能的肝实质样细胞的mRNA,通过基因芯片技术检测二者基因表达的变化,利用火山图及DAVID软件分析差异表达基因与细胞功能的相互关系.结果 与人胚肝脏前体细胞相比,肝实质样细胞中1 878个核苷酸序列表达上调,1 441个核苷酸序列表达下调.表达上调的基因主要涉及糖代谢、凋亡调节、血管发育、细胞迁移、蛋白磷酸化等生物学过程;表达下调的基因则与细胞周期、DNA代谢、细胞骨架的组装、细胞周期的调控、染色体的分离有关.结论 肝实质样细胞中与肝实质细胞功能相关的基因表达明显增加,而与细胞周期调控的基因表达下降.
目的 比較分析人肝髒前體細胞定嚮肝實質細胞分化過程中基因錶達的變化. 方法 分彆提取體外培養的人胚肝髒前體細胞及體外誘導分化後具有功能的肝實質樣細胞的mRNA,通過基因芯片技術檢測二者基因錶達的變化,利用火山圖及DAVID軟件分析差異錶達基因與細胞功能的相互關繫.結果 與人胚肝髒前體細胞相比,肝實質樣細胞中1 878箇覈苷痠序列錶達上調,1 441箇覈苷痠序列錶達下調.錶達上調的基因主要涉及糖代謝、凋亡調節、血管髮育、細胞遷移、蛋白燐痠化等生物學過程;錶達下調的基因則與細胞週期、DNA代謝、細胞骨架的組裝、細胞週期的調控、染色體的分離有關.結論 肝實質樣細胞中與肝實質細胞功能相關的基因錶達明顯增加,而與細胞週期調控的基因錶達下降.
목적 비교분석인간장전체세포정향간실질세포분화과정중기인표체적변화. 방법 분별제취체외배양적인배간장전체세포급체외유도분화후구유공능적간실질양세포적mRNA,통과기인심편기술검측이자기인표체적변화,이용화산도급DAVID연건분석차이표체기인여세포공능적상호관계.결과 여인배간장전체세포상비,간실질양세포중1 878개핵감산서렬표체상조,1 441개핵감산서렬표체하조.표체상조적기인주요섭급당대사、조망조절、혈관발육、세포천이、단백린산화등생물학과정;표체하조적기인칙여세포주기、DNA대사、세포골가적조장、세포주기적조공、염색체적분리유관.결론 간실질양세포중여간실질세포공능상관적기인표체명현증가,이여세포주기조공적기인표체하강.
Objective To identify the genes playing a functional role in differentiation of human hepatic progenitor cells to hepatocytes by comparing the gene expression and functional profiles of the two cell types.Methods mRNA was isolated from human fetal hepatic progenitor cells (hFHPCs) and functional hepatocyte-like cells (HLCs) that had differentiated from hFHPCs.Global gene expression profiling was performed on triplicate samples of each cell type.The differential gene expression was analyzed using volcano plot filtering and functional annotation was performed using the Database for Annotation, Visualization, and Integrated Discovery (DAVID).Results Compared to the hFHPCs, the HLCs had a total of 1878 significantly up-regulated genes and 1441 significantly down-regulated genes.The up-regulated genes included functional groups related to the hexose metabolic process, positive regulation of apoptosis, angiogenesis, regulation of cell motion, and protein amino acid phosphorylation.The down-regulated genes included functional groups related to cell cycle, DNA metabolic process, cytoskeleton organization, regulation cell cycle, and chromosome segregation.Conclusion Differentiation of HLCs from hFHPCs may involve increased expression of genes related to hepatocyte function and decreased expression of genes related to cell cycle regulation.
