中华肝胆外科杂志
中華肝膽外科雜誌
중화간담외과잡지
Chinese Journal of Hepatobiliary Surgery
2015年
11期
751-754
,共4页
马瑞红%乔铁%罗小兵%罗振亮%郑培明%杨柳青
馬瑞紅%喬鐵%囉小兵%囉振亮%鄭培明%楊柳青
마서홍%교철%라소병%라진량%정배명%양류청
华支睾吸虫%胆囊结石%寄生虫感染%扫描电镜%胆色素结石%碳酸钙结石
華支睪吸蟲%膽囊結石%寄生蟲感染%掃描電鏡%膽色素結石%碳痠鈣結石
화지고흡충%담낭결석%기생충감염%소묘전경%담색소결석%탄산개결석
Clonorchis sinensis%Gallbladder stone%Parasitic infection%Scanning electron microscope%Bile pigment stone%Calcium carbonate stone
目的 分析华支睾吸虫感染与胆囊结石类型的关系,为胆囊结石的防治提供依据.方法 对2011年5月至2014年9月广州南沙区第六人民医院普外科的1052例胆囊结石进行分析,采用研碎镜检法研究结石.将胆囊结石分为华支睾吸虫卵阳性组和阴性组,再用傅里叶红外光谱法分析结石主要成分,从而判断结石类型.随机抽取部分结石进行扫描电镜观察.结果 华支睾吸虫卵阳性结石300例,其中胆固醇、胆色素、碳酸钙、混合型和其他类型结石的例数和比例分别为28例(9.3％)、102例(34.0％)、102例(34.0％)、50例(16.7％)、18例(6.0％);华支睾吸虫卵阴性结石752例.其中上述类型结石的例数和比例分别为414例(55.1％)、132例(17.6％)、66例(8.8％)、94例(12.5％)、46例(6.1％).扫描电镜观察显示华支睾吸虫卵表面纹理的网孔吸附了很多细小颗粒,并与胆红素颗粒、碳酸钙晶体或磷酸盐、硬脂酸钙、蛋白的颗粒相互黏附.结论 华支睾吸虫卵阳性结石以胆色素和碳酸钙为主,华支睾吸虫卵阴性结石以胆固醇类结石为主,华支睾吸虫感染主要与胆色素和碳酸钙类结石有关.
目的 分析華支睪吸蟲感染與膽囊結石類型的關繫,為膽囊結石的防治提供依據.方法 對2011年5月至2014年9月廣州南沙區第六人民醫院普外科的1052例膽囊結石進行分析,採用研碎鏡檢法研究結石.將膽囊結石分為華支睪吸蟲卵暘性組和陰性組,再用傅裏葉紅外光譜法分析結石主要成分,從而判斷結石類型.隨機抽取部分結石進行掃描電鏡觀察.結果 華支睪吸蟲卵暘性結石300例,其中膽固醇、膽色素、碳痠鈣、混閤型和其他類型結石的例數和比例分彆為28例(9.3％)、102例(34.0％)、102例(34.0％)、50例(16.7％)、18例(6.0％);華支睪吸蟲卵陰性結石752例.其中上述類型結石的例數和比例分彆為414例(55.1％)、132例(17.6％)、66例(8.8％)、94例(12.5％)、46例(6.1％).掃描電鏡觀察顯示華支睪吸蟲卵錶麵紋理的網孔吸附瞭很多細小顆粒,併與膽紅素顆粒、碳痠鈣晶體或燐痠鹽、硬脂痠鈣、蛋白的顆粒相互黏附.結論 華支睪吸蟲卵暘性結石以膽色素和碳痠鈣為主,華支睪吸蟲卵陰性結石以膽固醇類結石為主,華支睪吸蟲感染主要與膽色素和碳痠鈣類結石有關.
목적 분석화지고흡충감염여담낭결석류형적관계,위담낭결석적방치제공의거.방법 대2011년5월지2014년9월엄주남사구제륙인민의원보외과적1052례담낭결석진행분석,채용연쇄경검법연구결석.장담낭결석분위화지고흡충란양성조화음성조,재용부리협홍외광보법분석결석주요성분,종이판단결석류형.수궤추취부분결석진행소묘전경관찰.결과 화지고흡충란양성결석300례,기중담고순、담색소、탄산개、혼합형화기타류형결석적례수화비례분별위28례(9.3％)、102례(34.0％)、102례(34.0％)、50례(16.7％)、18례(6.0％);화지고흡충란음성결석752례.기중상술류형결석적례수화비례분별위414례(55.1％)、132례(17.6％)、66례(8.8％)、94례(12.5％)、46례(6.1％).소묘전경관찰현시화지고흡충란표면문리적망공흡부료흔다세소과립,병여담홍소과립、탄산개정체혹린산염、경지산개、단백적과립상호점부.결론 화지고흡충란양성결석이담색소화탄산개위주,화지고흡충란음성결석이담고순류결석위주,화지고흡충감염주요여담색소화탄산개류결석유관.
Objective To study the relationship between Clonorchis sinensis infestation and different types of gallbladder stones.Methods From May 2011 to September 2014, 1 052 cases of gallbladder stones were collected from the Department of General Surgery at The Sixth People's Hospital of Nansha, Guangzhou.These stones were first grinded for microscopic examination and divided into two groups based on the results of detection of Clonorchis sinensis eggs.They were then analyzed by FTIR spectroscopy to identify the type of gallbladder stones.Some stones were also chosen randomly for observation under scanning electron microscope (SEM).Results Clonorchis sinensis eggs were found in 300 stones and among these, the number and proportion of cholesterol, bile pigment, calcium carbonate, mixed and other types of stones were 28 (9.3％), 102 (34.0％), 102 (34.0％), 50 (16.7％), and 18 (6.0％), respectively.In the 752 egg-negative stones, the number and proportion of the above five types of stones were 414 (55.1％), 132 (17.6％), 66 (8.8％), 94 (12.5％), and 46 (6.1％), respectively.Observation under SEM showed a lot of tiny particles were absorbed on the mesh of the superficial texture of the Clonorchis sinensis eggs, which were also adherent to the bilirubin particles, calcium stearate crystals, phosphate, calcium stearate and protein particles.Conclusions The main types of egg-positive stones were bile pigment and calcium carbonate stones, while cholesterol stone was the main type of egg-negative stones.Clonorchis sinensis infestation was associated mainly with bile pigment and calcium carbonate stones.
