实用药物与临床
實用藥物與臨床
실용약물여림상
Practical Pharmacy and Clinical Remedies
2015年
11期
1351-1355
,共5页
金蝉花%麦角甾醇%提取工艺%HPLC%星点设计-效应面法
金蟬花%麥角甾醇%提取工藝%HPLC%星點設計-效應麵法
금선화%맥각치순%제취공예%HPLC%성점설계-효응면법
Cordyceps cicadae%Ergosterol%Extraction technology%HPLC%Central composite design-response sur-face methodology
目的 优选金蝉花中麦角甾醇的最佳提取工艺. 方法 选择乙醇浓度、提取温度和提取时间为自变量,麦角甾醇提取量为因变量,在单因素试验基础上,采用星点设计-效应面法优选提取工艺,通过Design-Expert 8. 0. 6统计分析软件对试验数据进行多元线性模型和二次多项式模型拟合,并绘制效应面图和等高线图.结果 金蝉花中麦角甾醇的最佳提取工艺为以88%乙醇为提取溶剂,在74 ℃下提取38 min;麦角甾醇检测浓度在9. 28~148. 50 μg/mL范围内与其峰面积呈良好的线性关系,平均加样回收率为97. 99%. 结论 优化的条件简单、快速,可为金蝉花的进一步开发利用和有效性研究提供理论依据.
目的 優選金蟬花中麥角甾醇的最佳提取工藝. 方法 選擇乙醇濃度、提取溫度和提取時間為自變量,麥角甾醇提取量為因變量,在單因素試驗基礎上,採用星點設計-效應麵法優選提取工藝,通過Design-Expert 8. 0. 6統計分析軟件對試驗數據進行多元線性模型和二次多項式模型擬閤,併繪製效應麵圖和等高線圖.結果 金蟬花中麥角甾醇的最佳提取工藝為以88%乙醇為提取溶劑,在74 ℃下提取38 min;麥角甾醇檢測濃度在9. 28~148. 50 μg/mL範圍內與其峰麵積呈良好的線性關繫,平均加樣迴收率為97. 99%. 結論 優化的條件簡單、快速,可為金蟬花的進一步開髮利用和有效性研究提供理論依據.
목적 우선금선화중맥각치순적최가제취공예. 방법 선택을순농도、제취온도화제취시간위자변량,맥각치순제취량위인변량,재단인소시험기출상,채용성점설계-효응면법우선제취공예,통과Design-Expert 8. 0. 6통계분석연건대시험수거진행다원선성모형화이차다항식모형의합,병회제효응면도화등고선도.결과 금선화중맥각치순적최가제취공예위이88%을순위제취용제,재74 ℃하제취38 min;맥각치순검측농도재9. 28~148. 50 μg/mL범위내여기봉면적정량호적선성관계,평균가양회수솔위97. 99%. 결론 우화적조건간단、쾌속,가위금선화적진일보개발이용화유효성연구제공이론의거.
Objective To optimize the extraction process of ergosterol in cordyceps cicadae. Methods Based on single factor tests,central composite design-response surface methodology was applied to optimize the process with ethanol concentration,extraction temperature and extraction time as independent variables,and the extraction amounts of ergosterol as dependent variable. Design-Expert 8. 0. 6 statistic analysis software was used to fit multivariate linear equa-tion and second-order polynomial equation for experimental data, and delineate response surface and contour plot. Results The best technical condition was as follows:the extraction lasted for 38 min under 74 ℃ with 88% alco-hol as the extration solvent. The linear range of ergosterol was 9. 28~148. 50 μg/mL and the average recovery rate was 97. 99%. Conclusion The method is simple and rapid,and it provides scientific basis for the safety and effectiveness of cordyceps cicadae in clinical use.
