CAJ | 학술논문

目的探讨七氟烷预处理对失血性休克猪血浆肠脂肪酸结合蛋白( I-FABP)、D-乳酸浓度和细菌移位的影响. 方法巴马小型猪24头,雌雄不拘,采用随机数字表法均分为对照( C)组、失血性休克( S)组和七氟烷预处理( Sev)组. Sev组吸入七氟烷并保持2%呼出浓度30 min,C组和S组吸入空气30 min. 之后S组和Sev组制备失血性休克模型. 分别于麻醉前( T0 )、休克后30 min( T1 )、1 h( T2 )、1. 5 h( T3 )、2 h( T4 )、3 h( T5 )和4 h( T6 ) ,应用ELISA法测定各组血浆D-乳酸和I-FABP含量. 于T6 时点取肠组织行HE染色,取下腔静脉血、肠系膜淋巴结及肝、肺、肾、脾组织行细菌培养,并计算远隔器官细菌移位率. 结果 Sev组血浆D-乳酸、I-FABP浓度和远隔器官细菌移位率均明显低于S组(P<0. 05),明显高于C组(P<0.05). HE染色显示,S组肠黏膜损伤严重,Sev较之明显减轻,C组肠黏膜结构正常. 结论七氟烷预处理可显著降低失血性休克猪血浆I-FABP、D-乳酸浓度和细菌移位,对肠黏膜屏障具有保护作用.
목적 탐토칠불완예처리대실혈성휴극저혈장장지방산결합단백( I-FABP)、D-유산농도화세균이위적영향. 방법 파마소형저24두,자웅불구,채용수궤수자표법균분위대조( C)조、실혈성휴극( S)조화칠불완예처리( Sev)조. Sev조흡입칠불완병보지2%호출농도30 min,C조화S조흡입공기30 min. 지후S조화Sev조제비실혈성휴극모형. 분별우마취전( T0 )、휴극후30 min( T1 )、1 h( T2 )、1. 5 h( T3 )、2 h( T4 )、3 h( T5 )화4 h( T6 ) ,응용ELISA법측정각조혈장D-유산화I-FABP함량. 우T6 시점취장조직행HE염색,취하강정맥혈、장계막림파결급간、폐、신、비조직행세균배양,병계산원격기관세균이위솔. 결과 Sev조혈장D-유산、I-FABP농도화원격기관세균이위솔균명현저우S조(P<0. 05),명현고우C조(P<0.05). HE염색현시,S조장점막손상엄중,Sev교지명현감경,C조장점막결구정상. 결론 칠불완예처리가현저강저실혈성휴극저혈장I-FABP、D-유산농도화세균이위,대장점막병장구유보호작용.
Objective To investigate the effect of sevoflurane preconditioning on the concentration of plasma I-FABP and D-lactate and bacterial translocation in hemorrhagic shock pigs. Methods Twenty four Bama miniature pigs with male and female informal were equally divided into control ( C) group,hemorrhagic shock ( S) group and sevoflurane precondition ( Sev) group using random number table method. Pigs from group Sev inhaled 2% sevoflurane 30 min while animals from group C and group S inhaled air. Afterwards, the hemorrhagic shock model was established in group S and group Sev. The concentration of plasma I-FABP and D-lactate were measured using ELISA method be-fore anesthesia ( T0 ) and at 30 min ( T1 ) ,1 h ( T2 ) ,1. 5 h ( T3 ) ,2 h ( T4 ) ,3 h ( T5 ) ,4 h ( T6 ) after the hemorrhagic shock model was made. The jejunum sample was obtained for HE staining at T6 ,and the inferior vena venous blood, mesenteric lymph nodes, live, lung, kidney and spleen samples were obtained for bacterial culture at the same time. Afterwards,the bacterial translocation rate was calculated. Results The concentration of plasma D-lactate, I-FABP and the rate of bacterial translocation in group Sev were lower than those of group S (P<0. 05) and higher than those of group C (P<0. 05). HE staining showed obvious intestinal mucosa injury in group S. The injury was allevia-ted in group Sev while the tissue structure in group C was normal. Conclusion Sevoflurane preconditioning can signif-icantly decrease the concentration of plasma I-FABP,D-lactate and bacterial translocation,and has gut mucosal barrier protection effect on hemorrhagic shock pigs.