中华眼底病杂志
中華眼底病雜誌
중화안저병잡지
Chinese Journal of Ocular Fundus Diseases
2015年
6期
597-599
,共3页
基因疗法%预测%计算生物学%糖尿病视网膜病变/病理生理学
基因療法%預測%計算生物學%糖尿病視網膜病變/病理生理學
기인요법%예측%계산생물학%당뇨병시망막병변/병리생이학
Gene therapy%Forecasting%Computational biology%Diabetic retinopathy/physiopathology
目的 对hsa-miRNA-451进行靶基因预测及相关生物信息学分析.方法 选择miRBase、miRanda、TargetScan、PicTar数据库或预测软件预测hsa-miRNA-451靶基因.对预测的hsa-miRNA-451靶基因的基因集合进行功能富集分析和信号转导通路富集分析.采用Fisher精确概率法计算P值,P＜0.05为显著性阈值.结果 至少3个数据库或预测软件预测到hsa-miRNA-451靶基因的靶点18个.功能富集分析预测的结果显示,hsa-miRNA-451靶基因主要参与上皮细胞增生、发育以及调节酶活性相关生物过程(p＜0.05).信号转导通路富集分析预测的结果显示,has-miRNA-451靶基因显著富集于转化生长因子-β信号通路、丝裂原活化蛋白激酶信号通路、表皮生长因子信号通路、Wnt信号通路、哺乳动物雷帕霉素靶蛋白信号通路等(P＜0.05).结论 hsa-miRNA451可调控与上皮细胞增生、发育相关的多个信号通路.
目的 對hsa-miRNA-451進行靶基因預測及相關生物信息學分析.方法 選擇miRBase、miRanda、TargetScan、PicTar數據庫或預測軟件預測hsa-miRNA-451靶基因.對預測的hsa-miRNA-451靶基因的基因集閤進行功能富集分析和信號轉導通路富集分析.採用Fisher精確概率法計算P值,P＜0.05為顯著性閾值.結果 至少3箇數據庫或預測軟件預測到hsa-miRNA-451靶基因的靶點18箇.功能富集分析預測的結果顯示,hsa-miRNA-451靶基因主要參與上皮細胞增生、髮育以及調節酶活性相關生物過程(p＜0.05).信號轉導通路富集分析預測的結果顯示,has-miRNA-451靶基因顯著富集于轉化生長因子-β信號通路、絲裂原活化蛋白激酶信號通路、錶皮生長因子信號通路、Wnt信號通路、哺乳動物雷帕黴素靶蛋白信號通路等(P＜0.05).結論 hsa-miRNA451可調控與上皮細胞增生、髮育相關的多箇信號通路.
목적 대hsa-miRNA-451진행파기인예측급상관생물신식학분석.방법 선택miRBase、miRanda、TargetScan、PicTar수거고혹예측연건예측hsa-miRNA-451파기인.대예측적hsa-miRNA-451파기인적기인집합진행공능부집분석화신호전도통로부집분석.채용Fisher정학개솔법계산P치,P＜0.05위현저성역치.결과 지소3개수거고혹예측연건예측도hsa-miRNA-451파기인적파점18개.공능부집분석예측적결과현시,hsa-miRNA-451파기인주요삼여상피세포증생、발육이급조절매활성상관생물과정(p＜0.05).신호전도통로부집분석예측적결과현시,has-miRNA-451파기인현저부집우전화생장인자-β신호통로、사렬원활화단백격매신호통로、표피생장인자신호통로、Wnt신호통로、포유동물뢰파매소파단백신호통로등(P＜0.05).결론 hsa-miRNA451가조공여상피세포증생、발육상관적다개신호통로.
Objective To predict as well as bioinforrmatically analyze the target genes of has-miR-451.Methods miRBase, miRanda, TargetScan and PicTar were used to predict the target genes of hsa-miRNA-451.The functions of the target genes were demonstrated by Gene Ontology and pathway enrichment analysis.P＜0.05 was set as statistically significant.Results 18 target spots of hsa-miRNA-451 were predicted by 3 databases or prediction software at least.The functions of the target genes were enriched in proliferation and development of epithelial cells and regulation of kinase activity (P＜ 0.05).Pathway analysis showed that transforming growth factor-beta signaling pathway, mitogen-activated protein kinase signaling pathway, epidermal growth factor signaling pathway, Wnt signaling pathway and mammalian target of rapamycin signaling pathway were significantly enriched (P＜0.05).Conclusion hsa-miRNA-451 might be involved in various signaling pathways related to proliferation and development of epithelial cells.
