CAJ | 학술논문

目的比较结直肠癌患者与健康人群粪便菌群及代谢产物的差异.方法分别采用焦磷酸测序和气相色谱质谱联用(GC/MS)的代谢指纹技术分析15例结直肠癌患者和12例健康人粪便菌群和代谢产物变化.结果结直肠癌患者与正常人群粪便菌群多样性和均匀度的差异并无统计学意义(P＞0.05),但在菌种/属水平则有18种细菌相对丰度的差异有统计学意义(P＜0.05);GC/MS技术能清晰区分结直肠癌患者和正常人粪便代谢轮廓变化,结直肠癌患者粪便短链脂肪酸代谢产物醋酸、戊酸、丁酸、异丁酸、异戊酸及9种氨基酸显著高于健康人群(P＜0.05),而3种不饱和脂肪酸、2种甘油及熊去氧胆酸则明显低于正常人(P＜0.05).相关性分析表明,粪便菌群与代谢谱变化之间有很强的关联.结论结直肠癌患者与正常人粪便代谢产物及某些细菌种类存在明显差异,深入研究有助于了解肠道菌群参与结直肠癌发生的机制和发现结直肠癌新的诊断及治疗靶点.
목적 비교결직장암환자여건강인군분편균군급대사산물적차이.방법 분별채용초린산측서화기상색보질보련용(GC/MS)적대사지문기술분석15례결직장암환자화12례건강인분편균군화대사산물변화.결과 결직장암환자여정상인군분편균군다양성화균균도적차이병무통계학의의(P＞0.05),단재균충/속수평칙유18충세균상대봉도적차이유통계학의의(P＜0.05);GC/MS기술능청석구분결직장암환자화정상인분편대사륜곽변화,결직장암환자분편단련지방산대사산물작산、무산、정산、이정산、이무산급9충안기산현저고우건강인군(P＜0.05),이3충불포화지방산、2충감유급웅거양담산칙명현저우정상인(P＜0.05).상관성분석표명,분편균군여대사보변화지간유흔강적관련.결론 결직장암환자여정상인분편대사산물급모사세균충류존재명현차이,심입연구유조우료해장도균군삼여결직장암발생적궤제화발현결직장암신적진단급치료파점.
Objective To compare fecal microbiota and metabolites between colorectal cancer (CRC) patients and healthy population.Methods Feces from fifteen CRC patients and twelve normal people were analyzed by using pyrosequencing and gas chromatography mass spectrometry (GC/MS).Results There were no significant differences in the overall microbial community structure associated with the disease state, but 18 bacterial genera were underrepresented or overrepresented in the CRC samples.GC-MS profiling revealed higher concentrations for 9 kinds of amino acids and metabolites of short-chain fatty acids, lower concentrations for 3 kinds of unsaturated fatty acids and 2 kinds of glycerin and ursodeoxycholic acid in stool samples from CRC patients.Correlative analysis between the combined datasets revealed some potential relationships between stool metabolites and certain bacterial species.Conclusions There are significant differences in fecal metabolites and the relative abundance of certain types of bacteria between CRC patients and healthy people, which can provide insight into microbial functions occurring in a cancer environment and will help direct future mechanism studies.