CAJ | 학술논문

目的:探讨补阳还五汤对局灶性脑缺血大鼠海马区血管新生的影响.方法:采用大脑中动脉线栓法建立局灶性脑缺血大鼠模型,将动物随机分为正常组、假手术组、模型组、尼莫地平组和补阳还五汤组,给予不同处理,分别于1 d、7 d、14 d和28 d处死动物,采用免疫组织化学法观察海马区血管新生情况.结果:正常脑内海马区存在一定的BrdU、vWF阳性细胞,脑缺血7 d达高峰,然后逐渐下降;补阳还五汤组可显著增强BrdU的表达,在第7 d、14 d时明显多于其他各组(P<0.05).结论:补阳还五汤可促进局灶性脑缺血大鼠海马区血管新生.
목적:탐토보양환오탕대국조성뇌결혈대서해마구혈관신생적영향.방법:채용대뇌중동맥선전법건립국조성뇌결혈대서모형,장동물수궤분위정상조、가수술조、모형조、니막지평조화보양환오탕조,급여불동처리,분별우1 d、7 d、14 d화28 d처사동물,채용면역조직화학법관찰해마구혈관신생정황.결과:정상뇌내해마구존재일정적BrdU、vWF양성세포,뇌결혈7 d체고봉,연후축점하강;보양환오탕조가현저증강BrdU적표체,재제7 d、14 d시명현다우기타각조(P<0.05).결론:보양환오탕가촉진국조성뇌결혈대서해마구혈관신생.
Objective:To explore the effect of Buyang Huanwu Decoction(BYHWD) on angiogenesis in hippocampus of rats after focal cerebral ischemia.Methods:The model of focal cerebral ischemia in rats was reproduced by middle cerebral artery occlusion.Rats were randomly divided into following groups including navie, shame-operated, ischemia, Nimodipine and BYHWD groups. Animals were BrdU-labeled and killed at 1d, 7d, 14d and 28d after operation. BrdU and vWF were measured by immunohistochemical staining.Result: There was a certain BrdU or vWF positive cells in hippocampus of normal brain, after cerebral ischemia BrdU positive cells were increased and reached the peak at 7d.BYHWD can significantly increase the expression of BrdU or vWF.The increase was statistically significant at 7d and 14d after ischemia(P<0.05).Conclusion:BYHWD may promote the angiogenesis in hippocampus of rats after focal cerebral ischemia.