CAJ | 학술논문

目的:探讨黄甘颗粒对复发性生殖器疱疹HSV-2病毒的潜伏相关转录体(Latency-associated tranccrpts,LATs)的干预作用及对病毒滴度的影响.方法:采用雌性豚鼠阴道接种HSV-ⅡSav标准株,制作豚鼠RGH模型,随机分为三组分别进行干预,最终进行荧光定量PCR检测LATs拷贝量及检测病毒滴度,比较组间差异.结果:黄甘颗粒与泛昔洛韦均能明显减少RGH豚鼠模型LATs拷贝量,与生理盐水组比较均有明显差异,具有统计学意义(P<0.05).黄甘颗粒与泛昔洛韦均能明显降低病毒滴度数,两者比较无明显差异,无统计学意义(P>0.05);而两组与生理盐水组比较均有显著差异,有统计学意义(P<0.05).结论:中药黄甘颗粒具有益气解毒的作用,不仅从中医理论上能抗RGH复发,从分子生物学上研究,亦能明显减少RGH豚鼠模型LATs拷贝量,降低病毒滴度,达到减少病毒潜伏感染的细胞数量,起到预防RGH复发的作用,其具体机制及在临床上的应用还有待进一步研究.
목적:탐토황감과립대복발성생식기포진HSV-2병독적잠복상관전록체(Latency-associated tranccrpts,LATs)적간예작용급대병독적도적영향.방법:채용자성돈서음도접충HSV-ⅡSav표준주,제작돈서RGH모형,수궤분위삼조분별진행간예,최종진행형광정량PCR검측LATs고패량급검측병독적도,비교조간차이.결과:황감과립여범석락위균능명현감소RGH돈서모형LATs고패량,여생리염수조비교균유명현차이,구유통계학의의(P<0.05).황감과립여범석락위균능명현강저병독적도수,량자비교무명현차이,무통계학의의(P>0.05);이량조여생리염수조비교균유현저차이,유통계학의의(P<0.05).결론:중약황감과립구유익기해독적작용,불부종중의이론상능항RGH복발,종분자생물학상연구,역능명현감소RGH돈서모형LATs고패량,강저병독적도,체도감소병독잠복감염적세포수량,기도예방RGH복발적작용,기구체궤제급재림상상적응용환유대진일보연구.
Objective: To explore Huanggan granule's effect on HSV-2 latency associated transcripts and virus titer in treating recurrent genital herpes. Methods: RGH guinea-pig models were established by inoculating standard HSV-ⅡSav strains into the vagina of female guinea-pigs. The models were randomized into three groups, LATs copies and virus titer were detected by fluorescence quantitative PCR, and the difference was compared.Results: Both Huanggan granule and Famciclovir could decrease LATs copies contents of RGH guinea-pig models significantly, which had a significant difference when compared with that of saline group, the difference was statistically significant (P<0.05); Both Huanggan granule and Famciclovir could decrease virus titer, which had no significant difference and the difference was not statistically significant (P>0.05); when compared with that of saline group, the difference was statistically significant (P<0.05);Conclusion: Huanggan granule had a function of replenishing qi and detoxicating, which could be anti RGH recurrence and decrease LATs copies contents and virus titer of RGH guinea-pig models significantly, however, its concrete mechanism needed further clinical research.