CAJ | 학술논문

以平陆食用百合脱毒试管苗的鳞片为外植体 ,研究了不同接种部位和方式 ,不同植物激素种类和浓度对鳞片分化的影响.结果表明 :平陆百合鳞片的分化能力为外层>中层>内层 ,同一鳞片的不同部位分化能力为下段>中段>上段.接种方式以鳞片竖直插入培养基中为好 ,鳞片分化快 ,分化率高.试验中设置的培养基均可诱导鳞片分化 ,但附加不同种类和浓度的激素对鳞片分化出的小鳞茎的数量和生长状况影响不同 ,差异很大.其中以培养基MS+6-BA 0.8 mg·L -1 +NAA 0.1 mg·L -1上诱导出的小鳞茎数量多 ,生长壮 ,适宜作为快繁时获取大量鳞茎的培养基 ;在培养基MS+ZT 1.0 mg·L -1 +NAA 0.2 mg·L -1和MS+ZT 1.5 mg·L -1 + IBA 0.1 mg·L -1上诱导出的小鳞茎数量不多 ,但生长健壮 ,可直接成苗 ,适宜作为诱导鳞片分化较大鳞茎的培养基 ;诱导鳞片分化愈伤组织的最适宜培养基为MS+2 ,4-D 1.0 mg·L -1 +6-BA 0.5 mg·L -1 ,愈伤组织不经转换培养基可直接分化鳞茎.
이평륙식용백합탈독시관묘적린편위외식체 ,연구료불동접충부위화방식 ,불동식물격소충류화농도대린편분화적영향.결과표명 :평륙백합린편적분화능력위외층>중층>내층 ,동일린편적불동부위분화능력위하단>중단>상단.접충방식이린편수직삽입배양기중위호 ,린편분화쾌 ,분화솔고.시험중설치적배양기균가유도린편분화 ,단부가불동충류화농도적격소대린편분화출적소린경적수량화생장상황영향불동 ,차이흔대.기중이배양기MS+6-BA 0.8 mg·L -1 +NAA 0.1 mg·L -1상유도출적소린경수량다 ,생장장 ,괄의작위쾌번시획취대량린경적배양기 ;재배양기MS+ZT 1.0 mg·L -1 +NAA 0.2 mg·L -1화MS+ZT 1.5 mg·L -1 + IBA 0.1 mg·L -1상유도출적소린경수량불다 ,단생장건장 ,가직접성묘 ,괄의작위유도린편분화교대린경적배양기 ;유도린편분화유상조직적최괄의배양기위MS+2 ,4-D 1.0 mg·L -1 +6-BA 0.5 mg·L -1 ,유상조직불경전환배양기가직접분화린경.
Using the test tube flake seedings of Lilium brownii var .viridulum as explants ,we studied different inocula-tion sites and methods ,the effect of different plant hormones species and concentrations on the differentiation of scales . The results showed that :The differentiation ability of Scale in Lilium brownii var .viridulum was :Outer > middle> inner layer ,the ability to different parts of the same scale was :Upper> middle> section and the vertical scale in-serts the culture medium ,the differentiation and differentiation rats were the best .The Experiment seted all of the me-dium that could induce the differentiation of scales .The number and growth status of the small bulbs which were dif-ferentiated from the scales were differed greatly ,because of the different kinds and concentrations of hormones .MS+6-BA 0.8 mg·L -1 +NAA 0.1 mg·L -1 was induced the large number of small bulbs ,growth and strong ,the medium was suitable for the development of a large number of bulbs in the rapid propagation .The number of small bulbs in-duced by MS+ZT 1.0 mg·L -1 +NAA 0.2 mg·L -1 and MS+ZT 1.5 mg·L -1 +IBA 0.1 mg·L -1 was not much ,but strong greatly and directly seeding ,suitable as a medium for inducing the differentiation of the larger bulbs .The most suitable medium was MS+2 ,4-D1.0 mg·L -1 +6-BA 0.5 mg·L -1 which induced scale differentiation of callus ,the cal-lus without conversion medium direct differentiation of the bulb .