CAJ | 학술논문

研究蔓越莓对中波紫外线(UVB)诱导的人表皮角质形成细胞(HaCaT)光损伤的保护作用.用3个不同剂量的蔓越莓果汁预处理HaCaT细胞6 h,采用60 mJ/cm2强度UVB照射细胞;然后用MTT法检测细胞的生存率,吸取细胞上清液采用比色法检测丙二醛(MDA)、羟脯氨酸(Hyp)的含量和超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、乳酸脱氢酶(LDH)的活性变化,用荧光法检测细胞内活性氧自由基(ROS)含量,用倒置显微镜和流式细胞仪观察检测细胞凋亡状况.UVB辐射对HaCaT细胞造成比较严重的损伤; 相比于空白对照组,UVB辐射模型组HaCaT细胞活性下降了29.54%,SOD和GSH-Px活性极显著降低(P<0.01);相比于模型组,蔓越莓各剂量组可显著提高UVB照射后HaCaT细胞的活力(P<0.05或P<0.01),提高SOD和GSH-Px活性(P<0.01),减少MDA和ROS含量(P<0.05或P<0.01),并且降低LDH活性和增加Hyp含量(P<0.01),呈剂量依赖关系,从而降低UVB所导致的细胞损伤及凋亡率升高.蔓越莓可以明显减少UVB诱导HaCaT细胞的氧化损伤和凋亡,具有显著的光保护功效,其作用机制与增强细胞抗氧化能力、加速清除氧自由基以及促进胶原蛋白合成有关.
연구만월매대중파자외선(UVB)유도적인표피각질형성세포(HaCaT)광손상적보호작용.용3개불동제량적만월매과즙예처리HaCaT세포6 h,채용60 mJ/cm2강도UVB조사세포;연후용MTT법검측세포적생존솔,흡취세포상청액채용비색법검측병이철(MDA)、간포안산(Hyp)적함량화초양화물기화매(SOD)、곡광감태과양화물매(GSH-Px)、유산탈경매(LDH)적활성변화,용형광법검측세포내활성양자유기(ROS)함량,용도치현미경화류식세포의관찰검측세포조망상황.UVB복사대HaCaT세포조성비교엄중적손상; 상비우공백대조조,UVB복사모형조HaCaT세포활성하강료29.54%,SOD화GSH-Px활성겁현저강저(P<0.01);상비우모형조,만월매각제량조가현저제고UVB조사후HaCaT세포적활력(P<0.05혹P<0.01),제고SOD화GSH-Px활성(P<0.01),감소MDA화ROS함량(P<0.05혹P<0.01),병차강저LDH활성화증가Hyp함량(P<0.01),정제량의뢰관계,종이강저UVB소도치적세포손상급조망솔승고.만월매가이명현감소UVB유도HaCaT세포적양화손상화조망,구유현저적광보호공효,기작용궤제여증강세포항양화능력、가속청제양자유기이급촉진효원단백합성유관.
To study the photo-protection effect of cranberry on HaCaT cells damaged by ultraviolet radiation B (UVB). HaCaT cells were first incubated for six hours with three different doses of cranberry juice, and then irradiated with 60 mJ/cm2 dose of UVB. The cell viability was detected by the MTT method. The level of malondialdehyde (MDA), hydroxyproline (Hyp) and the activites of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), lactate dehydrogenase (LDH) of supernatants were determined with the colorimetric methods. The content of intra-cellular reactive oxygen species (ROS) was tested with the fluorescence method. Compared with the control group, the UVB irradiation could seriously damage HaCaT cells, which lead to 29.54%decline in cell viability and obvious decrease in SOD and GSH-Px activities (P<0.01). Importantly, compared with the UVB model group, cranberry could obviously enhance cell viability (P<0.05 or P<0.01), increase SOD, GSH-Px activities and Hyp content, decrease LDH activity and the contents of MDA and ROS in supernatants under UVB irradiation in dose-dependent manner (P<0.01 or P<0.05), which lead to the decreased rate in cell damage and apoptosis. In conclusion , cranberry could relieve UVB-induced oxidative damage and apoptosis of HaCaT cells and exhibit the photo-protection effect , which might be the reasons that cranberryefficiently enhanced the oxidase activity , cleared the oxy-radicals and stimulated the collagen synthesis in cells.